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A Sensitive “Turn-On” Fluorescent Sensor for Melamine Based on FRET Effect between Polydopamine-Glutathione Nanoparticles and Ag Nanoparticles

  • Li Tang
    Li Tang
    Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
    More by Li Tang
  • Shi Mo
    Shi Mo
    Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
    More by Shi Mo
  • Shi Gang Liu
    Shi Gang Liu
    Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
    More by Shi Gang Liu
  • Yu Ling
    Yu Ling
    Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
    More by Yu Ling
  • Xiao Fang Zhang
    Xiao Fang Zhang
    Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
    More by Xiao Fang Zhang
  • Nian Bing Li*
    Nian Bing Li
    Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
    *E-mail: [email protected]
    More by Nian Bing Li
  • , and 
  • Hong Qun Luo*
    Hong Qun Luo
    Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
    *E-mail: [email protected]. Fax: (+86)23-6825-3237. Tel: (+86)23-6825-3237.
    More by Hong Qun Luo
Cite this: J. Agric. Food Chem. 2018, 66, 9, 2174–2179
Publication Date (Web):February 8, 2018
https://doi.org/10.1021/acs.jafc.7b05245
Copyright © 2018 American Chemical Society

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    Abstract

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    In this work, Ag nanoparticles (AgNPs) were synthesized quickly by a one-step method utilizing polydopamine-glutathione nanoparticles (PDA-GNPs) as a reducing agent. The PDA-GNPs and the generated AgNPs acted as the energy donor and acceptor, respectively. Accordingly, the fluorescence of PDA-GNPs was quenched on the basis of fluorescence resonance energy transfer (FRET). In the presence of melamine, the preferential combination of Ag(I) and melamine to form Ag(I)–melamine complex prevents Ag(I) from forming AgNPs, together with fluorescence enhancement compared with the absence of melamine. Under the optimal conditions including the concentration of AgNO3, reaction time, reaction temperature, and pH, the fluorescence enhancement efficiency has a linear response to the concentration of melamine from 0.1 to 40 μM with a detection limit of 23 nM for melamine. The proposed method is simple, time-saving, and low-cost, which was further applied to detect melamine in real milk products with satisfactory results.

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.jafc.7b05245.

    • Comparison of proposed method with other fluorescence methods for the detection of melamine, effect of increasing AgNO3 concentration on fluorescence intensity, effect of reaction temperature and reaction time on fluorescence intensity, effect of media pH on fluorescence intensity, stability of fluorescence enhancement efficiency, and supplemental references (PDF)

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    19. Martin Zeilinger, Hermann Sussitz, Wim Cuypers, Christoph Jungmann, Peter Lieberzeit. Mass-Sensitive Sensing of Melamine in Dairy Products with Molecularly Imprinted Polymers: Matrix Challenges. Sensors 2019, 19 (10) , 2366. https://doi.org/10.3390/s19102366
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