Green tea extracts decrease carcinogen-induced mammary tumor burden in rats and rate of breast cancer cell proliferation in culture
Kathryn T. Kavanagh
Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Kathryn T. Kavanagh and Dong W. Kim were the recipients of the Karin Grunebaum Cancer Research Fellowship.
Search for more papers by this authorLaurie J. Hafer
Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Search for more papers by this authorDong W. Kim
Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118
Kathryn T. Kavanagh and Dong W. Kim were the recipients of the Karin Grunebaum Cancer Research Fellowship.
Search for more papers by this authorKoren K. Mann
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Department of Environmental Health, Boston University School of Public Health, Boston, Massachusetts 02118
Search for more papers by this authorDavid H. Sherr
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Department of Environmental Health, Boston University School of Public Health, Boston, Massachusetts 02118
Search for more papers by this authorAdrianne E. Rogers
Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Search for more papers by this authorCorresponding Author
Gail E. Sonenshein
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118
Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118.Search for more papers by this authorKathryn T. Kavanagh
Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Kathryn T. Kavanagh and Dong W. Kim were the recipients of the Karin Grunebaum Cancer Research Fellowship.
Search for more papers by this authorLaurie J. Hafer
Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Search for more papers by this authorDong W. Kim
Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118
Kathryn T. Kavanagh and Dong W. Kim were the recipients of the Karin Grunebaum Cancer Research Fellowship.
Search for more papers by this authorKoren K. Mann
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Department of Environmental Health, Boston University School of Public Health, Boston, Massachusetts 02118
Search for more papers by this authorDavid H. Sherr
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Department of Environmental Health, Boston University School of Public Health, Boston, Massachusetts 02118
Search for more papers by this authorAdrianne E. Rogers
Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Search for more papers by this authorCorresponding Author
Gail E. Sonenshein
Program in Research on Women's Health, Boston University School of Medicine, Boston, Massachusetts 02118
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118
Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118.Search for more papers by this authorAbstract
Epidemiological evidence suggests tea (Camellia sinensis L.) has chemopreventive effects against various tumors. Green tea contains many polyphenols, including epigallocatechin-3 gallate (EGCG), which possess anti-oxidant qualities. Reduction of chemically induced mammary gland carcinogenesis by green tea in a carcinogen-induced rat model has been suggested previously, but the results reported were not statistically significant. Here we have tested the effects of green tea on mammary tumorigenesis using the 7,12-dimethylbenz(a)anthracene (DMBA) Sprague-Dawley (S-D) rat model. We report that green tea significantly increased mean latency to first tumor, and reduced tumor burden and number of invasive tumors per tumor-bearing animal; although, it did not affect tumor number in the female rats. Furthermore, we show that proliferation and/or viability of cultured Hs578T and MDA-MB-231 estrogen receptor-negative breast cancer cell lines was reduced by EGCG treatment. Similar negative effects on proliferation were observed with the DMBA-transformed D3-1 cell line. Growth inhibition of Hs578T cells correlated with induction of p27Kip1 cyclin-dependent kinase inhibitor (CKI) expression. Hs578T cells expressing elevated levels of p27Kip1 protein due to stable ectopic expression displayed increased G1 arrest. Thus, green tea had significant chemopreventive effects on carcinogen-induced mammary tumorigenesis in female S-D rats. In culture, inhibition of human breast cancer cell proliferation by EGCG was mediated in part via induction of the p27Kip1 CKI. J. Cell. Biochem. 82:387–398, 2001. © 2001 Wiley-Liss, Inc.
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