Abstract
Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A watson-Crick G-C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.
Publication types
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, Non-P.H.S.
- Research Support, U.S. Gov't, P.H.S.
MeSH terms
- Base Composition
- Base Sequence
- Conserved Sequence
- Escherichia coli
- Molecular Sequence Data
- Mutagenesis
- Nucleic Acid Conformation
- Peptidyl Transferases / metabolism*
- RNA, Bacterial / metabolism
- RNA, Ribosomal, 23S / genetics
- RNA, Ribosomal, 23S / metabolism*
- RNA, Transfer / metabolism*
- Ribosomes / metabolism*
Substances
- RNA, Bacterial
- RNA, Ribosomal, 23S
- RNA, Transfer
- Peptidyl Transferases