Abstract
The influence of hypotonic solution on cell volume and electrophysiology properties of the failing heart of cardiomyopathic hamsters (TO-2) was investigated. The results showed an increase in cell volume of quiescent isolated ventricular myocytes by 66% within 30 min. Angiotensin (1–7) [Ang (1–7)] (10−8 M) administered to isotonic solution, elicited a gradual decline in cell volume and a significant decrease of the swelling-activated chloride current (I Clswell). The effect of Ang (1–7) on cell volume was inhibited by ouabain (10−7 M). Angiotensin II (10−8 M) caused cell swelling and increased I Clswell. Experiments performed on isolated left ventricles of cardiomyopathic hamsters at an advanced stage of the disease, indicated that hypotonic solution prepared by diluting the normal Krebs solution by 25%, showed a gradual decrease of conduction velocity, generation of early after depolarizations and block of impulse conduction within 10 min. Implications to myocardial ischemia are discussed.
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This work was supported by grant HL-34148 from NIH.
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De Mello, W.C. Cell swelling, impulse conduction, and cardiac arrhythmias in the failing heart. Opposite effects of angiotensin II and angiotensin (1–7) on cell volume regulation. Mol Cell Biochem 330, 211–217 (2009). https://doi.org/10.1007/s11010-009-0135-0
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DOI: https://doi.org/10.1007/s11010-009-0135-0