Isolation of microsatellite markers in Cannabis sativa L. (marijuana)

S. Gilmore,

Corresponding Author

S. Gilmore

Centre for Forensic Science, Canberra Institute of Technology, PO Box 826, Canberra ACT 2601, Australia,

School of Botany and Zoology, Australian National University, Canberra ACT 0200, Australia

S. Gilmore. Fax: +61-2-62074105; E-mail: [email protected]Search for more papers by this author

R. Peakall,

R. Peakall

School of Botany and Zoology, Australian National University, Canberra ACT 0200, Australia

Search for more papers by this author

S. Gilmore,

Corresponding Author

S. Gilmore

Centre for Forensic Science, Canberra Institute of Technology, PO Box 826, Canberra ACT 2601, Australia,

School of Botany and Zoology, Australian National University, Canberra ACT 0200, Australia

S. Gilmore. Fax: +61-2-62074105; E-mail: [email protected]Search for more papers by this author

R. Peakall,

R. Peakall

School of Botany and Zoology, Australian National University, Canberra ACT 0200, Australia

Search for more papers by this author

First published: 20 January 2003

https://doi.org/10.1046/j.1471-8286.2003.00367.x

Citations: 43

Read the full text

About

PDF

Tools

Share a link

Email
Facebook
Twitter
LinkedIn
Reddit
Wechat

Abstract

We have identified 15 variable microsatellite loci in Cannabis sativa. In 48 samples from five fibre crop seed accessions, we detected an average of 10 alleles per locus (range 2–28) with mean heterozygosity of 0.68 (range 0.28–0.94). Significant genetic differentiation was found between accessions (F_ST = 0.12, P < 0.001). These markers have utility for characterizing genetic diversity in cultivated and naturalized Cannabis populations.

References

Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus, 12, 13–15.
10.1046/j.1471-8286.2002.00210.x
Google Scholar
Fischer D, Bachmann K (1998) Microsatellite enrichment in organisms with large genomes (Allium cepa L.). Biotechniques, 24, 796–802.

CASPubMedWeb of Science®Google Scholar
Peakall R, Gilmore S, Keys W, Morgante M, Rafalski A (1998) Cross-species amplification of Soybean (Glycine max) simple sequence repeats (SSRs) within the genus and other legume genera: implications for the transferability of SSRs in plants. Molecular Biology and Evolution, 15, 1275–1287.
10.1093/oxfordjournals.molbev.a025856
CASPubMedWeb of Science®Google Scholar
Peakall R, Smouse P (2001) genalex V5: Genetic Analysis in Excel. Australian National University, Canberra, Australia. http://www.anu.edu.au/BoZo/GenAlEx/.

Web of Science®Google Scholar
Rozen S, Skaletsky HJ (1997) PRIMER 3 code available at http://www-genome.wi.mit.edu/genome_software/other/primer3.html.

Google Scholar
Schuelke M (2000) An economic method for the fluorescent labelling of PCR fragments. Nature Biotechnology, 18, 233–234.
10.1038/72708
CASPubMedWeb of Science®Google Scholar
Scott IA, Hayes CM, Keogh JS, Morrison SF (2001) Isolation and characterisation of novel microsatellite markers from the Australian water skink Eulamprus kosciuskoi and cross-species amplification in other members of the species-group. Molecular Ecology Notes, 1, 28–30.
10.1046/j.1471-8278.2000.00011.x
CASWeb of Science®Google Scholar
Small E, Cronquist A (1976) A practical and natural taxonomy for Cannabis. Taxon, 25, 405–435.
10.2307/1220524
Web of Science®Google Scholar

Citing Literature

Volume3, Issue1

March 2003

Pages 105-107

Isolation of microsatellite markers in Cannabis sativa L. (marijuana)

Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Isolation of microsatellite markers in Cannabis sativa L. (marijuana)

Abstract

References

Citing Literature

References

Related

Information