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mutations in the BMP 15 gene (Belli and Shimasaki, 2018). However, the significance of the BMP 15 gene in the fertility of Bos indicus cattle has received little attention. Until now, the BMP 15 gene was found to be polymorphic in... more
mutations in the BMP 15 gene (Belli and Shimasaki, 2018). However, the significance of the BMP 15 gene in the fertility of Bos indicus cattle has received little attention. Until now, the BMP 15 gene was found to be polymorphic in Peranakan Ongole (PO) and Bengali cattle which is categorized as one of Zebu cattle (Bos indicus
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Objective: As one of the most valuable genetic resources of Ongole beef cattle globally, the Sumba Ongole (SO) cattle population is being studied in this investigation of myostatin (MSTN) gene polymorphism and its association with growth... more
Objective: As one of the most valuable genetic resources of Ongole beef cattle globally, the Sumba Ongole (SO) cattle population is being studied in this investigation of myostatin (MSTN) gene polymorphism and its association with growth traits. Materials and Methods: Blood samples from 161 SO cattle were collected and analyzed. Deoxyribonucleic acid (DNA) was isolated. The DNA was electrophoresed and extracted, and finally, the annealing temperature was optimized, followed by amplification and sequencing. Next, we used a Basic local alignment search tool to assess the sequencing data. Results: The analysis revealed 22 single nucleotide polymorphisms (SNPs) in the MSTN gene in this region that showed genetic variation. Two SNPs, c.424 G > A, and c.467 G > C, were found to be significantly associated with SO cattle phenotypes of wither height, heart girth, and hip height (p < 0.05) but not with body weight or body length (p > 0.05). Conclusion: As a result of our findings...
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Penelitian mengenai studi performa umum dan status fisiologis kuda Sumba di dilaksanakan pada tanggal April-September 2018 bertempat di Peternakan kuda sumba, Pulau Sumba, NTT. Tujuan penelitian ini adalah untuk mengetahui performa umum... more
Penelitian mengenai studi performa umum dan status fisiologis kuda Sumba di dilaksanakan pada tanggal April-September 2018 bertempat di Peternakan kuda sumba, Pulau Sumba, NTT. Tujuan penelitian ini adalah untuk mengetahui performa umum kuda sumba dalam kaitannya dengan status fisiologisnya. Penelitian yang digunakan adalah metode deskriptif yaitu metode yang menggambarkan objek sesuai dengan apa adanya, pengambilan sampel dilakukan selama dua minggu. Dalam penelitian ini peubah yang diamati adalah frekuensi respirasi, frekuensi denyut jantung dan suhu tubuh yang dilakukan pada 30 ekor indukan kuda sumba memiliki riwayat pernah digunakan untuk olahraga kuda pacuan baik kuda dengan jenis kelamin jantan dan betina dan 5 ekor anakan. Hasil dari penelitian tersebut disimpulkan bahwa frekuensi respirasi indukan memiliki nilai rataan 33±6.7 per menit dan anak memiliki nilai rataan 44±5.7 per menit. Frekuensi denyut jantung indukan memiliki nilai rataan 44±8.0 per menit dan 62±2.8 per meni...
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Testis is the male reproductive organ that performs both endocrine and exocrine functions. This study aimed to determine the distribution of carbohydrates in the testis of sumba ongole cattle. The sample consisted of six testis were... more
Testis is the male reproductive organ that performs both endocrine and exocrine functions. This study aimed to determine the distribution of carbohydrates in the testis of sumba ongole cattle. The sample consisted of six testis were collected from East Sumba Slaughter House, fixed in formalin 10 %, processed histologically, and continued with AB pH 2,5 - PAS staining. The result showed a weak alcian blue intensity (+) in the tunica albuginea and moderate intensity (++) in the interstitial connective tissue, basement membrane, and connective tissue in the lumen of the seminiferous tubules. A strong intensity (+++) to PAS staining was seen in spermatogonia, spermatocytes, and myoid cells. Spermatids, spermatozoa, and sertoli cells showed a negative reaction (-). In the intertubular area, Leydig cells and fibroblasts showed a strong PAS reaction (+++), connective tissue showed a moderate reaction (++). The intensity of the weak reaction (+) is indicated by the basement membrane of the ...
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This research aims to study testis and epididymis of dry land goat (Capra hircus) on B-mode ultrasound imaging. Twelve adult local male goats were examined to study normal testes and epididymis ultrasonic appearances. B-mode real time... more
This research aims to study testis and epididymis of dry land goat (Capra hircus) on B-mode ultrasound imaging. Twelve adult local male goats were examined to study normal testes and epididymis ultrasonic appearances. B-mode real time scanner with a 7.5 MHz, linear array transducer was used to evaluate the echotexture of the testicular parenchyma, mediastinum and the epididymis. The testicular parenchyma was represented uniformly homogenous and moderately echogenic with the mediastinum was more highly echogenic. The epididymal head appeared less echogenic but homogenous in structure, while the epididymal tail was more heterogeneous and less echogenic than the testis. Each structure in male reproductive organ, the testis, head and tail of epididymis can be differentiated using ultrasound imaging, but body epididymis was difficult to identify. It is concluded that ultrasound can be as a valuable diagnostic tool to investigate the health of male goat reproductive organ.
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The aims of this study is to determine the quality of fresh cement of Landrace pigs diluted with natural diluent of green coconut water. The research was conducted at the Veterinary Reproduction Laboratory of FKKH Undana. Fresh semen of... more
The aims of this study is to determine the quality of fresh cement of Landrace pigs diluted with natural diluent of green coconut water. The research was conducted at the Veterinary Reproduction Laboratory of FKKH Undana. Fresh semen of Landrace pigs was collected from male pigs that less than 3 years sexually mature and in healthy condition, then the semen quality was examined by macroscopic and microscopic evaluation. Only cement met the requirements, the proportion of spermatozoa motility not less than 70%, spermatozoa concentration not less than 200 million cells/ml and spermatozoa abnormality not more than 20%. After that, the semen was diluted by adding green coconut water and antibiotics according to the dilution formula. The results showed that semen diluted with natural green coconut was better and could be used as an alternative diluent with a higher of spermatozoa viability and motility (48.2% & 41.25%) compared to fresh semen with a shelf life of 20 hours.
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This study aims to determine the antioxidant effect of the ethanol extract of the leaves of Moringa (Moringa oleifera Lam.) In water diluent palm fruit of the quality of landrace pig spermatozoa. This study uses a Completely Randomized... more
This study aims to determine the antioxidant effect of the ethanol extract of the leaves of Moringa (Moringa oleifera Lam.) In water diluent palm fruit of the quality of landrace pig spermatozoa. This study uses a Completely Randomized Design (CRD) with 5 treatments, 1 control dan 4 repetitions and observation of the motility and viability of spermatozoa performed every 2 hours until a decline in the percentage motility of at least 40%. The cement used in the Laboratory of Technical Services Unit for Breeding and Forage at Tarus, Kupang. Escrow done every two weeks from the male landrace pigs who have experienced sexual maturity. Landrace pig semen storage results were evaluated macroscopically and microscopically. Good quality cement had ≥70% motility, concentration ≥200x106 sel spermatozoa/ml and abnormalities of ≤20%. Cement is added to the water diluent palm fruit then added with antioxidants. Antioxidants should be added during the storage process, sperm metabolic activity prod...
The aims of this study is to determine the effect and the best combination of coconut water (CW) and native chicken egg yolk (NCEY) as extender with water jacket and non-water jacket method in preserving of boar semen. Semen was... more
The aims of this study is to determine the effect and the best combination of coconut water (CW) and native chicken egg yolk (NCEY) as extender with water jacket and non-water jacket method in preserving of boar semen. Semen was collected from sexualy mature boar 2-4 years of age. A good sample of semen will have motility above 70%, abnormality below 20% and spermatozoa consentration above 200 x 106 sel/mL. This study was designed with completly randomized design and faktorial patern with 2 controled group, 6 treated group, and 4 repitition. Controled group consist of fresh semen without extender. K0 was preserved with non-water jacket method, and K1 was preserved with water jacket method. Treated group were P1 (75% CW + 25% NCEY), P2 (85% CW + 15% NCEY), P3 (95% CW + 5% NCEY) that preserved with non-water jacket method, and P4 (75% CW + 25% NCEY), P5 (85% CW + 15% NCEY), P6 (95% CW + 5% NCEY) preserved with water jacket method. Motility and viability of spermatozoa were evalu...
The aim of this study is to find the best concentration ratio of native chicken egg yolk (NCEY) and lontar water (LW) in order to preserve landrace boar semen, with water jacket and non-water jacket method, at 5°C and to find how long can... more
The aim of this study is to find the best concentration ratio of native chicken egg yolk (NCEY) and lontar water (LW) in order to preserve landrace boar semen, with water jacket and non-water jacket method, at 5°C and to find how long can it preserve the semen. Fresh semen was collected from Landrace boar 2-4 years old age. Semen sample is categorized apropriate to be extended if >70% sperm motility, >200x106 cells/ mL, and >20% sperm abnormality. This study was designed with compeltely ranomized design and faktorial patern with 2 controled group, 6 treated group, and 4 repitition. Controled group consist of fresh semen without extender. K0 was preserved with non-water jacket method, and K1 was preserved with water jacket method. Controled group were P1 (95% LW + 5% NCEY), P2 (85% LW + 15% NCEY), P3 (75% LW + 25% NCEY) that preserved with water jacket method, and P4 (95% LW + 5% NCEY), P5 (85% LW + 15% NCEY), P6 (75% LW + 25% NCEY) preserved with non-water jacket method. Sp...
Camplong II is one of the villages in the Kupang regency dominated by cattle farmers with limited knowledge and skill in managing animal waste. These farmers have the habit of burning organic material that can be used as a fertilizer... more
Camplong II is one of the villages in the Kupang regency dominated by cattle farmers with limited knowledge and skill in managing animal waste. These farmers have the habit of burning organic material that can be used as a fertilizer source, which adversely affects the environment. Therefore, this study aims to improve the knowledge and skills of farmers by training them to make organic fertilizer from a natural compound such as animal manure. Furthermore, the purpose of implementing this community service is to manufacture organic bokashi using organic fertilizers and vegetable products. Other important aspects affecting this community are the level of education, the role of farmers, and the source of information. The result showed that community service activities have provided positive benefits on organic fertilizer and skills in the form of increased knowledge by producing bokashi fertilizer. The final product has benefited from improving household income for farmers in Camplong...
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Pertumbuhan populasi babi di Indonesia disetiap daerah dari tahun ke tahun mengalami peningkatan. Testis berfungsi menghasilkan spermatozoa dan hormon testosteron. Penelitian ini bertujuan untuk mengetahui pengaruh ukuran testis terhadap... more
Pertumbuhan populasi babi di Indonesia disetiap daerah dari tahun ke tahun mengalami peningkatan. Testis berfungsi menghasilkan spermatozoa dan hormon testosteron. Penelitian ini bertujuan untuk mengetahui pengaruh ukuran testis terhadap kualitas spermatozoa babi Landrace dan babi Duroc dengan umur berkisar 2-4 tahun. Penelitian yang digunakan adalah penelitian eksperimental meliputi pengukuran testis dan evaluasi semen secara makroskopis dan mikroskopis. Data mikroskopis yang diperoleh dianalisis secara deskriptif. Hasil penelitian rerata evaluasi semen babi Landrace secara mikroskopis menunjukkan motilitas 82.5±0.00 % dan viabilitas 88.7±0.00 %, sedangkan evaluasi makroskopis semen babi Duroc menunjukka motilitas 82.5±0.00 % dan viabilitas 88.6±0.00 %. Hasil rerata ukuran panjang testis babi Landrace bagian kiri 12.66 ± 3.0 cm, bagian kanan 14.00 ± 1.0 cm, lebar testis bagian kiri 7.66 ± 2.081 cm, bagian kanan 8.00±0.00 cm. Babi Duroc memiliki panjang testis kiri 16.66 ± 5.507 cm,...
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Sandelwood horse is one of local horses in Indonesia that has become known for its use in horse racing competition. Many genes are known to have an effect on performances traits, but the role of one gene, Myostatin (MSTN) has not been... more
Sandelwood horse is one of local horses in Indonesia that has become known for its use in horse racing competition. Many genes are known to have an effect on performances traits, but the role of one gene, Myostatin (MSTN) has not been explored yet. This gene is responsible for embryonic and adult muscle development. Due to the rare information about this gene characteristic, this study aimed to identify myostatin (MSTN) gene in Sandelwood horse in both male and female horses. The blood sample from five stallions and five mares which reared half wildly in Sumba Island were collected and analyzed. The method used in this research was polymerase chain reaction (PCR), electrophoresis and DNA Sequencing. Myostatin gene was successfully amplified with a temperature 60c for 463 bp. A total of 10 samples have been sequenced in PCR machine. The MSTN gene forward primer was 5’TATTCTTCTTGGGAGGGAGGACTACT-3’ and reverse primer was 5’GCAAGTAATTAGCACAAAAATTTGAATG-3’. The software Basic Alignment L...
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This study aims to determine the quality of Landrace porkspermatozoa with the addition of natural thinners of lontar fruit juice and Moringa oleifera Lam. Leaf extract as antioxidants stored at different storage temperatures, namely 5°C... more
This study aims to determine the quality of Landrace porkspermatozoa with the addition of natural thinners of lontar fruit juice and Moringa oleifera Lam. Leaf extract as antioxidants stored at different storage temperatures, namely 5°C (freezer),15°C (refrigerator) and 22°C (air-conditioned room). Semen was collected six times and evaluated both macroscopically and microscopically. The best quality of semen was semen with spermatozoa motility> 70%, concentration> 200x106 cells / ml and abnormal spermatozoa <20%. In this study, there were three treatments, in which the cement with antioxidant diluents was stored at 15°C. (treatment I), 22°C (treatment II) and 5 oC (treatment III). The results showed at the 36th hour of storage the temperature was 22°C with a motility of 55.00 ± 0.20% and a viability of 72.50 ± 0.27%, a temperature of 15°C with a motility of 55.00 ± 0.28% and a viability of 64.50 ± 0.20%, and a temperature of 5°C with a motility of 50.00 ± 0.50 % and viabili...
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The Sumba horse is one of the local horses in Indonesia which is known as racing horse Several candidate genes are known to influence the outward characteristics of the Sumba racehorse, which play main role in the development of the... more
The Sumba horse is one of the local horses in Indonesia which is known as racing horse Several candidate genes are known to influence the outward characteristics of the Sumba racehorse, which play main role in the development of the horse's muscles from embryo to adulthood. This research aims to identify candidate genes for the Sumba racehorse in stallion and mares. Blood samples from 5 stallions and 5 mares were collected and analyzed. The method used in this research was by using polymerase chain reaction (PCR), electrophoresis and DNA sequencing. The results of DNA amplification fragments at a temperature of 600c showed a fragment size of 463 bp. A total of 10 samples were sequenced on the PCR machine. The forward primer was 5'-TATTCTTCTTGGGAGGGAGGACTACT-3 'and reverse primer was 5'-GCAAGTAATTAGCACAAAAATTTGAATG-3'. The obtained data was analyzed using the Basic Local Alignment Sealing Tool (BLAST). Result of this study could be used as an initial identificatio...
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Sebagian besar masyarakat Desa Camplong II, Kec. Fatuleu, Kab. Kupang memiliki mata pencaharian sebagai petani dan peternak. Desa ini memiliki topografi landai, bergelombang dengan kondisi tanah berkapur yang dikelilingi berbagai macam... more
Sebagian besar masyarakat Desa Camplong II, Kec. Fatuleu, Kab. Kupang memiliki mata pencaharian sebagai petani dan peternak. Desa ini memiliki topografi landai, bergelombang dengan kondisi tanah berkapur yang dikelilingi berbagai macam tumbuhan yang menyebabkan Desa Camplong II memiliki sampah organik lebih banyak dibandingkan sampah anorganik. Akan tetapi, selama ini sampah belum diolah secara optimal sehingga tumpukan sampah yang sebenarnya bermanfaat bagi pertumbuhan tanaman lainnya dibiarkan begitu saja. Oleh karena itu, melalui program pengabdian ini, tim pengabdi mengajak masyarakat desa untuk mengolah sampah organik menjadi produk yang bermanfaat bagi pertanian dengan membuat pupuk organik cair (POC). Pupuk organik cair merupakan larutan yang dihasilkan dari pembusukan alami bahan organik yang memiliki unsur hara yang dapat menyuburkan dan menggemburkan lapisan tanah, dimana dapat membantu menyuburkan kondisi tanah di desa Campong II yang cenderung tidak dalam, mudah tereros...
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The objective of this study was to determine the the effect diluent of commercial is diluent BTS® and MIII® in maintaining sperm motility and viability of landrace boars during storage at different temperatures at refrigerator (5 °C), AC... more
The objective of this study was to determine the the effect diluent of commercial is diluent BTS® and MIII® in maintaining sperm motility and viability of landrace boars during storage at different temperatures at refrigerator (5 °C), AC (28 °C) and room temperature (32 °C). This experiment used a completely randomized design (CRD) with 6 treatment and 4 replications. Semen was collected twice a week from landrace boars. Semen Characteristic and their quality were evaluated macroscopically and microscopically. Good quality semen have motility >70%, sperm concentration 200 x 106 sperm/ml and morphological abnormality less than 20%, these semen were added with diluent commercial is diluent BTS® and MIII®. Sperm motility and viability were evaluated every 2 hours until 40% motility were evaluated. The result showed that fresh semen characteristic were good, with the presentage of sperm motility 85% with sperm concentration 242.50±16.5% and morphological abnormality 12.50±1.70%. The ...
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Bali cattle is one of the native cattle in Indonesia. High fertility and are capabled to adapt well in tropical environment is one of the superiority of bali cattle. Bali cattle farming in East Nusa Tenggara (NTT) are generally managed by... more
Bali cattle is one of the native cattle in Indonesia. High fertility and are capabled to adapt well in tropical environment is one of the superiority of bali cattle. Bali cattle farming in East Nusa Tenggara (NTT) are generally managed by intensive, semi-intensive and extensive system traditionaly, without any recording and lacking good breeding practices. The purpose of this research is to determine the reproduction performance of bali cattle on semi-intensive management system based on pregnancy rate, days open and calving interval. The number of cattles used in this study were 40 cows on age 4-7 years. The methods used were interviews and rectal palpation to know the reproductive status of livestock. Descriptive analysis was used in this research. The results showed that the pregnancy rate was 45%, days open was 112,5 days and calving interval was 13,075 months. This study indicated a decreased reproductive efficiency of bali cattle in Kupang Regency, hence an improvement of the ...
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Kuda Sandelwood merupakan aset lokal Pulau Sumba yang memiliki fungsi sosial dan ekonomi yang cukup tinggi. Profil kimia darah merupakan salah satu parameter yang dapat digunakan untuk penegakan diagnosis suatu penyakit yang dapat... more
Kuda Sandelwood merupakan aset lokal Pulau Sumba yang memiliki fungsi sosial dan ekonomi yang cukup tinggi. Profil kimia darah merupakan salah satu parameter yang dapat digunakan untuk penegakan diagnosis suatu penyakit yang dapat menyebabkan kerusakan organ, salah satunya hati. Salah satu pemeriksaan fungsi hati dapat dilakukan dengan pengujian enzim hati yang meliputi Serum Glutamic Oxaloacetic Transaminase (SGOT) atau aspartat aminotransferase (AST). Penelitian ini bertujuan untuk mengukur kadar SGOT/AST kuda Sandelwood secara umum maupun berdasarkan jenis kelamin. Pengambilan sampel dilakukan pada Desa Maubokul, Kecamatan Pandawai di Kabupaten Sumba Timur, kemudian pemeriksaan sampel dilakukan di UPT Laboratorium Kesehatan Provinsi NTT. Sampel serum darah diambil dari 20 ekor kuda Sandelwood (10 ekor jantan dan 10 ekor betina) yang sehat secara klinis kemudian serum diuji pada mesin Erba XL 300. Hasil yang didapat direratakan menggukan aplikasi SPSS 16. Hasil penelitian menunjuk...
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Normal hematological values need to be defined for each category of horse, like age and sex to obtain precise diagnostic results. No published data on hematology exist for Sandalwood horses that are extensively reared in East Sumba, NTT.... more
Normal hematological values need to be defined for each category of horse, like age and sex to obtain precise diagnostic results. No published data on hematology exist for Sandalwood horses that are extensively reared in East Sumba, NTT. The main objective of this research was to compare the hematological characteristic of horse group based on age and sex. Blood samples from sixteen horses were collected based on sex into male (n=6), female (n=10) and age groups of 2-3 years (n=4) and 6-10 years old (n=12). About 3 ml of blood sample was collected aseptically from jugular vein of each of these animals in sterile vacutainer tube. The following variables were measured: erythrocyte, leucocyte, platelet, hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and mean platelet volume (MPV). These hematological parameters were calculated with the automatic hematology analyzer at Health Laborator...
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Generally, real time, B-mode ultrasound scanner has become an essential part for veterinary reproduction applications. Diagnostic ultrasound seems to be a useful tool to study anatomical structures and to confirm echogenic pattern in... more
Generally, real time, B-mode ultrasound scanner has become an essential part for veterinary reproduction applications. Diagnostic ultrasound seems to be a useful tool to study anatomical structures and to confirm echogenic pattern in reproductive organ (Holman et al, 2011). Many experiments showed that ultrasonography imaging has considerable beneficial for the evaluation of the internal structure of reproductive organ function in domestic animals (Beal et al , 1992) as it can be used as a non-invasive technique to evaluate animal reproductive health (Holman et al 2011). Pregnancy detection with ultrasonography provides more advantage compare to manual palpation because of its ability to detect early presence of embryo and its accuracy (Beal et al , 1992; Nation et al 2003). To the best of our knowledge, most of cattle farmers and veterinarians in Maumere have relied on one single method for detecting pregnancy in cows, that is, rectal palpation. However, this method has its limitat...
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The study aims to determine the effect of commercial thinning is thinning Beltsville thawing Solution (BTS) and Mulberry III (MIII) with a water jacket method and non-water jacket against landrace pig semen quality after stored at 5° C.... more
The study aims to determine the effect of commercial thinning is thinning Beltsville thawing Solution (BTS) and Mulberry III (MIII) with a water jacket method and non-water jacket against landrace pig semen quality after stored at 5° C. This study uses a completely randomized design (CRD) with 4 treatments and 2 control within five repetitions. Fresh semen that fulfill the criteria diluted with Beltsville thawing Solution (BTS) and Mulberry III (MIII), then stored using non-water jacket and water jacket at 5 °C of temperature. Motility and viability observations of spermatozoa performed every 2 hours after storage for up to a decline in the percentage motility of at least 40%. Research result shows the characteristics of fresh semen motility is 84.50 ± 0:50, the concentration is 250 ± 8:36 sperm cells / ml and 13.60 ± 1.72 abnormality. Semen observations which diluted with Beltsville thawing Solution (BTS) at 5 °C temperature shows the water jacket method is better than the non-wat...
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Pigs are one of the potential livestock to breed with the aim of meeting the needs of animal protein, but in fact the level of pig production is not optimal due to the low efficiency of reproduction. One way that can be used to increase... more
Pigs are one of the potential livestock to breed with the aim of meeting the needs of animal protein, but in fact the level of pig production is not optimal due to the low efficiency of reproduction. One way that can be used to increase the productivity of sows that by superovulation method. This study aimed to determine the effects of PMSG hormone injections to increase the productivity level of sows that given superovulation treatment. 10 sows that have been selected, be divided into two research groups: control group and treatment group. Before the treatment is given in the form of PMSG hormone injections, sows from both study groups, first injected with hormones PGF2α to estrus synchronization purposes. Then, sows in the treatment group are injected by PMSG hormone with the dose of 2 ml/sow before naturally mating with the boar. After the mating process, the gestation period of pregnant sow will be observed until the parturition time. The parameters were litter size and the birt...
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This research was aimed to defined the morphology of boar’s sperm and using carbofuchsin staining. The semen sample were collected with technique glove hand method from three healty Landrace and Duroc boars belong to UPT pembibitan Ternak... more
This research was aimed to defined the morphology of boar’s sperm and using carbofuchsin staining. The semen sample were collected with technique glove hand method from three healty Landrace and Duroc boars belong to UPT pembibitan Ternak Babi Dinas Peternakan Provinsi NTT. Semen were stained using Carbofucsin staining technique, morphology observation was evaluated by microscop on 500 cells for each sample. From macroscopic and microscopic evaluation the result showed that the Landrace boar’s semen volume was308.33±23.63 ml, the pH was 7,4 and the sperm concentration was 230±20x106 cell/mL. Macroscopic and microscopic evaluation the result showed that the Duroc boar’s semen volume was 213.33±41.63 ml, the pH was 7,4 and the sperm concentration was 455±198.30x106 cell/mL. The result of morphology observation in Landrace and Duroc boars showed that the normal sperm was 95.91±1.14% and94.02±2.76%. Abnormal sperm in Landrace and Duroc was4.13±1.11% dan 5.97±2.76%. The abnormality of th...
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Pola perkawinan dan kelahiran menjadi salah satu faktor penting dari manajemen reproduksi pada sistem pemeliharan semi intensif. Penelitian ini dilakukan untuk mengetahui pola perkawinan dan estimasi kelahiran berdasarkan hasil diagnosis... more
Pola perkawinan dan kelahiran menjadi salah satu faktor penting dari manajemen reproduksi pada sistem pemeliharan semi intensif. Penelitian ini dilakukan untuk mengetahui pola perkawinan dan estimasi kelahiran berdasarkan hasil diagnosis kebuntingan sapi bali pada sistem pemeliharaan semi intensif padang penggembalaan di daerah lahan kering. Manfaat dari penelitian ini adalah sebagai data base dalam penerapan manajemen perkawinan. Sebanyak 208 ekor induk sapi bali di kelompok ternak Tamnau Amaf, Desa Bena, Kecamatan Amanuban Selatan, Kabupaten Timor Tengah Selatan, Provinsi NTT dilakukan anamnesa dan pemeriksaan per rektal pada tanggal 28-30 Mei 2019. Angka kebuntingan yang diketahui pada penelitian ini adalah 90.86 % (189/208). Persentase usia kebuntingan tertua adalah usia kebuntingan 7 bulan 26,96 % (56/208). Berdasarkan data kebuntingan diketahui puncak pola perkawinan terjadi pada bulan Oktober 2018 dan estimasi kelahiran mencapai puncak pada bulan Juli 2019. Perkawinan sapi se...
Some of the chemical compounds in bitter melon fruit that acts as infertility for males, namely saponins, tannins, alkaloids, flavonoids and triterpenoids were able to reduce the number of spermatogenic cells. Renal excretory function in... more
Some of the chemical compounds in bitter melon fruit that acts as infertility for males, namely saponins, tannins, alkaloids, flavonoids and triterpenoids were able to reduce the number of spermatogenic cells. Renal excretory function in carrying out this gets tough task, because almost 25% of all blood flow to the two kidneys. The amount of blood flow to the kidney causes renal exposure to the material circulating in the circulation system is quite high, so that the toxic material will easily cause damage to the kidney tissue. This study aimed to determine the effect of dose delivery infusion of local bitter melon fruit on the island of Timor, East Nusa Tenggara against renal histopathology description of male white rat (Rattus norvegicus). The study was conducted in the 2 treatment groups, namely (P1) bitter melon infusion at a dose of 1250 mg/kgBB/day and (P2) bitter melon infusion at a dose of 2500 mg/kgBB/day. Each treatment group consisted of 6 rats administered for 48 days. M...
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Hepatic is the largest gland in the body. Hepatic function in the metabolism and detoxification of a wide variety of compounds into the body. Infusion extract of bitter melon (M. charantia L.) fruit is a herbal preparation containing... more
Hepatic is the largest gland in the body. Hepatic function in the metabolism and detoxification of a wide variety of compounds into the body. Infusion extract of bitter melon (M. charantia L.) fruit is a herbal preparation containing chemical compounds such as flavonoids, glycosides, triterpenoids, polyphenols, alkaloids, saponins, and tannins. Excessive drug dosing in a long period of time, can make liver damage acute, subacute or chronic. Toxic effects of drugs often seen in the tissues, especially the liver and kidneys are on histology, appearing as inflammatory cell infiltration, hidropic degeneration, fatty degeneration and necrosis. The purpose of this study is to determine the effect of variations in dose infusion extract of bitter melon fruit (M. charantia L.) locally NTT against white picture rat liver histopathology (R. norvegicus) male and determine the effective dose infusion extract of bitter melon fruit (M. charantia L.) NTT local who gave a response, or effects on fer...
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The objective of this study was to determine the capability of coconut water, coconut water-honey, palmyra juice, palmyra juice-honey in maintaining sperm motility and viability of landrace boars during storage at refrigerator (5 °C )... more
The objective of this study was to determine the capability of coconut water, coconut water-honey, palmyra juice, palmyra juice-honey in maintaining sperm motility and viability of landrace boars during storage at refrigerator (5 °C ) and room temperature (32 °C). This experiment used a completely randomized design (CRD) with 8 treatments and 3 replications. Semen was collected twice a week from landrace boars. Semen Characteristics and their quality were evaluated macroscopically and microscopically. Good quality semen have motility >70%, sperm concentration >200x106 sperm/ml and morphological abnormalities less than 20%, these semen were added with Coconut Water (P1), Coconut Water-Honey (P2), palmyra juice (P3) and palmyra juice-honey (P4). Sperm motility and viability were evaluated every 2 hours until 40% motility were evaluated. The results showed that fresh semen characteristics were good, with the presentage of sperm motility 85%, sperm concentration 266.67 ± 13.33% ...
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The aim of this study was to investigate the effect of Siwalan Palm Juice as a natural extender for maintaining boar sperm quality. Semen ejaculates collected from three Duroc and Landrace boars were macroscopically (volume, colour, odor,... more
The aim of this study was to investigate the effect of Siwalan Palm Juice as a natural extender for maintaining boar sperm quality. Semen ejaculates collected from three Duroc and Landrace boars were macroscopically (volume, colour, odor, pH and consistency) and microscopically (motility, concentration and abnormality) evaluated. Semen samples fulfilled all criteria for semen characteristic (motility > 70%, concentration 200 x 106 cells /ml and abnormality ≤ 20%). Antibiotics were added in this semen samples. This study used completely randomized design with 6 treated groups, each group repeated three times. K0 (control): fresh semen; K1: Siwalan juice + Landrace boar semen; K2: Coconut water + Landrace boar semen; K3: NaCL + Landrace boar semen; K4: Siwalan juice + Duroc boar semen; K5: Coconut water + Duroc boar semen; K6: NaCL + Duroc boar semen. This semen samples were stored under room temperature at 220C. The result shows that Duroc and Landrace boar spermatozoa can be stor...
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Physiological status is an indicator to determine the degree of health and productivity of livestock. This study aims to identify the physiological status of Sumba Ongole (SO) beef cattle on the island of Sumba by measuring body... more
Physiological status is an indicator to determine the degree of health and productivity of livestock. This study aims to identify the physiological status of Sumba Ongole (SO) beef cattle on the island of Sumba by measuring body temperature, respiration frequency, pulse, and body conditioning score (BCS). Overall, there were 70 cows used in the study. Based on gender, there were 21 bulls and 49 cows and they were classified based on age, there were 3 categories, 1-1.5 years, 2-2.5 years, and 3 years. The parameters measured were body temperature, respiratory rate, pulse frequency, and BCS. The results of the study which were processed using analysis of variances (ANOVA) showed that there was no effect of sex or age on body temperature, respiration frequency, pulse frequency (P> 0.05), but there was a significant difference in the age category with the body score of SO cattle (P<0.05). It could be concluded that the physiological status of SO beef cattle which includes body tem...
A study was carried out in one of Indonesia native cattle, Sumba Ongole (SO) Cattle for determination of statuses of various biochemical parameters, aspartate aminotransferase (AST or SGOT) and alanine aminotransferase (ALT or SGPT).... more
A study was carried out in one of Indonesia native cattle, Sumba Ongole (SO) Cattle for determination of statuses of various biochemical parameters, aspartate aminotransferase (AST or SGOT) and alanine aminotransferase (ALT or SGPT). Apparently blood from randomly selected cattle (n= 36) were collected aseptically. Biochemical analysis of AST and ALT was performed on UPT. Laboratorium Kesehatan, Kupang, NTT. The results showed that the variations of parameters were not significant (p>0.05) between male and female SO cattle. The AST/SGOT (142.56±20.55 U/l) was slightly higher in female compared with the male (137.00±16.24 U/l), while level of ALT/SGPT were 46.44±10.99 U/l in male and 46.41 ± 10.23 U/l in female. Although, the result was not significant (p>0.05), level of AST and ALT in SO cattle was higher compared to standard which is 60-125 U/l for AST and 6.9-35 U/l for ALT. Results showed possibility of seasonal effect on AST/ SGOT and ALT/SGPT level in male and female SO c...
Tujuan dilaksanakan penelitian ini untuk melihat pengaruh metode penyimpanan preservasi yang berbeda terhadap viabilitas spermatozoa semen babi landrace dalam pengencer alami air buah lontar (AL) dengan penambahan kuning telur ayam... more
Tujuan dilaksanakan penelitian ini untuk melihat pengaruh metode penyimpanan preservasi yang berbeda terhadap viabilitas spermatozoa semen babi landrace dalam pengencer alami air buah lontar (AL) dengan penambahan kuning telur ayam kampung (KT). Semen diambil dari 5 ekor pejantan landrace yang telah dewasa kelamin. Setelah itu dilakukan pemeriksaan makros-mikros dari semen segar, semen dengan kriteria motilitas , konsentrasi dan abnormalitas spermatozoa berturt-turut: >70%, >200 juta spermatozoa/ml, <20% yang layak untuk dijadikan semen cair. Perlakuan dalam penelitian:, P1(AL 95%+5% KT), P2(AL 85%+15% KT), P3(AL 75%+25% KT) yang simpan pada metode water jacket (WJ) dan P4(AL 95%+5% KT), P5(AL 85%+15% KT), P6(AL 75%+25% KT) yang disimpan dengan metode non water jacket (NWJ), semen dikemas dalam mikrotube 1 ml dan disimpan pada suhu preservasi. Pada penelitian ini menunjukkan bahwa viabilitas spermatozooa dalam pengencer alami air buah lontar yang ditambahkan dengan kunin...
Timorese fruit bat(Pteropusvampyrus)is the only fly mammalian with its unique behavior which hanging upside down inspite of its pregnancy. This research is aimed to reveal the morphology of the Timorese fruit bats and the distribution of... more
Timorese fruit bat(Pteropusvampyrus)is the only fly mammalian with its unique behavior which hanging upside down inspite of its pregnancy. This research is aimed to reveal the morphology of the Timorese fruit bats and the distribution of neutral carbohydrate within this organ. Three uterus samples derived from three different Timorese fruit bats were used in the research.Both macroscopical and microscopical examinations using H&E and PAS methods were applied. Macroscopically, Timorese fruit bats showedsoft reddish white duplex uterus. Meanwhile microscopically, endometrium consisted of epithelial layer and lamina propria and was the place where simple tubular glands located. The epithelial layer comprised of simple cylindric secretory cells and ciliated cells. Neutral carbohydrate distribution was seen within this epithelial layer. Myometrium was a thick circular smooth muscle layer which consisted of smooth muscle separated by collagen and elastic fibre. Perimetrium was a visceral ...