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Analysis of Microsatellite Diversity in Ethiopian Tetraploid Wheat Landraces

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Abstract

The extent and patterns of microsatellite diversity in 141 Ethiopian tetraploid wheat landraces consisting of three species Triticum durum Desf., T. dicoccon Schrank and T. turgidum L. were analyzed using 29 microsatellite markers. A high level of polymorphism and a large number of alleles unique for each species were detected. Compared to emmer (T. dicoccon) and poulard (T. turgidum) wheats, a higher genetic diversity was observed in T. durum. The A-genome was more polymorphic than the B-genome in all the three species. Microsatellites with (GA) n -repeats had a higher number of alleles than (GT) n -repeats. A species pairwise comparison was made to determine the percentage of shared alleles and a large number of common alleles among species were observed. Average gene diversity, across the 29 microsatellite loci, was 0.684 for T. durum, 0.616 for T. dicoccon and 0.688 for T. turgidum. Genetic distances were lower between T. durum and T. turgidum (0.26) than between T. durum and T. dicoccon (0.34) or between T. turgidum and T. dicoccon (0.38). A significant correlation (p < 0.01) was found between the number of alleles per locus and the gene diversity in all the three species. Allelic frequency variation was highest between T. turgidum and T. dicoccon (10.62%) and lowest between T. durum and T. turgidum (4.86%). A genetic similarity coefficient of 0.34, 0.46 and 0.37 was found in T. durum, T. dicoccon, and T. turgidum, respectively. The dendogram, which was constructed on the basis of a similarity matrix using the UPGMA algorithm, distinguished all accessions represented in the study.

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Authors and Affiliations

  1. Institute of Crop Science, Agro-biodiversity Department, University of Kassel, Steinstrasse 19, 37213, Witzenhausen, Germany

    Yifru Teklu & K. Hammer

  2. Institut of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466, Gatersleben, Germany

    X.Q. Huang & M.S. Röder

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  1. Yifru Teklu
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  2. K. Hammer
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  3. X.Q. Huang
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  4. M.S. Röder
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Correspondence to Yifru Teklu.

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Teklu, Y., Hammer, K., Huang, X. et al. Analysis of Microsatellite Diversity in Ethiopian Tetraploid Wheat Landraces. Genet Resour Crop Evol 53, 1115–1126 (2006). https://doi.org/10.1007/s10722-005-1146-7

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  • DOI: https://doi.org/10.1007/s10722-005-1146-7

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