Abstract
A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.
Publication types
- Research Support, Non-U.S. Gov't
MeSH terms
- Animals
- Crosses, Genetic
- DNA Nucleotidyltransferases / genetics
- DNA Polymerase I / genetics
- Female
- GTP-Binding Proteins*
- Gene Targeting / methods*
- Genetic Vectors
- Integrases*
- Interferon-alpha / pharmacology
- Interferon-beta / pharmacology
- Liver / drug effects
- Liver / metabolism
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Inbred CBA
- Mice, Transgenic
- Myxovirus Resistance Proteins
- Poly I-C / pharmacology
- Promoter Regions, Genetic
- Proteins / genetics
- Recombination, Genetic
- Sequence Deletion
- Viral Proteins*
Substances
- Interferon-alpha
- Myxovirus Resistance Proteins
- Proteins
- Viral Proteins
- Interferon-beta
- Cre recombinase
- DNA Nucleotidyltransferases
- Integrases
- DNA Polymerase I
- GTP-Binding Proteins
- Poly I-C