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A candidate for Lr19, an exotic gene conditioning leaf rust resistance in wheat

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Abstract

Lr19, one of the few widely effective genes conferring resistance to leaf rust in wheat, was transferred from the wild relative Thinopyrum ponticum to durum wheat. Since Lr19 confers a hypersensitive response to the pathogen, it was considered likely that the gene would be a member of the major nucleotide-binding site (NBS)-leucine-rich repeat (LRR) plant R gene family. NBS profiling, based on PCR amplification of conserved NBS motifs, was applied to durum wheat–Th. ponticum recombinant lines involving different segments of the alien 7AgL chromosome arm, carrying or lacking Lr19. Differential PCR products were isolated and sequenced. From one such sequence (AG15), tightly linked to Lr19, a 4,121-bp full-length cDNA was obtained. Its deduced 1,258 amino acid sequence has the characteristic NBS-LRR domains of plant R gene products and includes a coiled-coil (CC) region typical of monocots. The genomic DNA sequence showed the presence of two exons and a short intron upstream of the predicted stop codon. Homology searches revealed considerable identity of AG15 with the cloned wheat resistance gene Pm3a and a lower similarity with wheat Lr1, Lr21, and Lr10. Quantitative PCR on leaf-rust-infected and non-infected Lr19 carriers proved AG15 to be constitutively expressed, as is common for R genes.

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Acknowledgments

Financial support from the Italian Ministry of Research, grant FISR (Fondo Integrativo Speciale per la Ricerca) 2005–2008 is gratefully acknowledged. The senior author acknowledges a Marie Curie fellowship grant to carry out part of this work. Thanks are due to Leslie Boyd, John Innes Centre, Norwich, UK, for advice in leaf rust infections, and to Douglas Knott, University of Saskatchewan, Saskatoon, Canada, and Robert McIntosh, Plant Breeding Institute, University of Sydney, Australia, for seeds of the Agatha mutants.

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  1. Department of Agrobiology and Agrochemistry, University of Tuscia, via S. Camillo de Lellis, 01100, Viterbo, Italy

    Andrea Gennaro & Carla Ceoloni

  2. Department of Crop Genetics, John Innes Centre, Norwich, NR4 7UH, UK

    Robert M. D. Koebner

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  1. Andrea Gennaro
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Correspondence to Carla Ceoloni.

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Fig. S1

Amino acid sequence comparison of proteins encoded by AG15 and Pm3a genes performed by ClustalW2 software using default parameters. Start positions of CC, NBS and LRR domains are indicated in bold. The AG15 CC domain, identified by Jpred 3 software, is underlined. Conserved motifs, characteristics of the NBS domain of NBS-LRR proteins (in the following order: P-loop, Kinase 2, RNBS-B, GLPL, MHD), are boxed and show complete identity between the two proteins. Intron position, identified in both AG15 (see text) and Pm3a (Yahiaoui et al. 2004) by comparison of the genomic sequences with the cDNA sequences, is conserved between the two genes and located immediately upstream of the stop codon (vertical arrow) (GIF 106 kb)

High resolution (EPS 1274 kb)

Fig. S2

Neighbor-joining phylogenetic tree of AG15 and related cereal proteins encoded by R genes or RGAs. Abbreviation of the Latin species name precedes the protein name (where known) and the accession number. Chromosome location of each sequence is reported in brackets. Proteins encoded by genes isolated in wheat are indicated in bold (GIF 13 kb)

High resolution (EPS 1274 kb)

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Gennaro, A., Koebner, R.M.D. & Ceoloni, C. A candidate for Lr19, an exotic gene conditioning leaf rust resistance in wheat. Funct Integr Genomics 9, 325–334 (2009). https://doi.org/10.1007/s10142-009-0115-1

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