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Interference of bio-control Trichoderma to enhance physical and physiological strength of sugarcane during Pokkah boeng infection

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Abstract

Tremendous benefits have been derived from the use of fungicides but excessive use of chemical fungicides not only posing threat to human and animal life but also contaminates the prevailing environment. Damage by pathogenic fungi alone causes significant damage to crops like maize, rice, wheat, soybeans, and potatoes. Therefore, it becomes imperative that these diseases are checked and controlled, for which chemical pesticides are being sprayed on plants extensively. Considering the devastating damage and toxicity, the global focus has taken a drift from synthetic chemicals to nature-friendly biological control agents. The present study focuses on the use of biological control agents particularly Trichoderma in sugarcane during Pokkah boeng infection. In the present experiment, twenty promising Trichoderma strains were evaluated for plant growth promotion, lytic enzymes, and physiological and biocontrol activity. Out of the twenty, four potential Trichoderma strains were assessed in the pot experiment viz. T. harzianum strain T28, T41 and T49 and T. aureoviride strain T38. The T. harzianum (T28) showed efficient plant growth-promoting traits as it produced IAA (20.67 µg/ml), phosphorus solubilization (18.57 µg/ml), and cell wall degrading enzymes such as chitinase (24.98 µg/ml) and β-glucanase (29.98 µg/ml). The interference of biocontrol agent T. harzianum (T28) controlled the disease by 73.55%. Apart from this, the inoculation of Trichoderma (T28) enhanced growth attributes including germination percentage (26.61%), mean tiller number (8.28 tiller/pot), individual cane length (241.5 cm), single cane weight (1.13 kg) and the number of milleable canes (6.00 cane/pot). Improvements in physiological activities at different growth stages of the sugarcane crop were observed based on the photosynthetically active radiation (PAR) on the leaf surface, transpiration rate, stomatal conductance, and photosynthetic rate. Further, improvement in juice quality parameters was also observed as it recorded the highest 0brix, sucrose, and commercial cane sugar by 21.26%, 19.28%, and 13.50%, respectively, by applying T. harzianum strain T28. Thus, results proved that T. harzianum strain T28 may be an effective eco-friendly biocontrol tool for managing Pokkah boeng disease in sugarcane. This is the first report of the biocontrol potential of Trichoderma spp. against Fusarium proliferatum causing Pokkah boeng disease in sugarcane.

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Acknowledgements

We express our sincere thanks to the Director, ICAR-Indian Institute of Sugarcane Research, Lucknow (U.P.), for continuous support and for providing us necessary facilities to conduct the studies. We also express our gratitude to AMITY Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Lucknow (U.P.) for providing the necessary support to take up research work.

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Authors and Affiliations

  1. ICAR-Indian Institute of Sugarcane Research, Lucknow, 226008, India

    Raghvendra Tiwari, S. K. Shukla, V. P. Jaiswal, Abhay Kumar Srivastava, Asha Gaur & Divya Sahni

  2. Birbal Sahni Institute of Palaeosciences, Lucknow, 226007, India

    Kajal Chandra

  3. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Surat, Gujarat, 394350, India

    Natarajan Amaresan

  4. Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Lucknow, 226028, India

    Raghvendra Tiwari & Rajesh Kumar Tiwari

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  1. Raghvendra Tiwari
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Tiwari, R., Chandra, K., Shukla, S.K. et al. Interference of bio-control Trichoderma to enhance physical and physiological strength of sugarcane during Pokkah boeng infection. World J Microbiol Biotechnol 38, 139 (2022). https://doi.org/10.1007/s11274-022-03319-z

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