Whole-Genome Shotgun Assembly and Analysis of the Genome of Fugu rubripes
Abstract
The compact genome of Fugu rubripes has been sequenced to over 95% coverage, and more than 80% of the assembly is in multigene-sized scaffolds. In this 365-megabase vertebrate genome, repetitive DNA accounts for less than one-sixth of the sequence, and gene loci occupy about one-third of the genome. As with the human genome, gene loci are not evenly distributed, but are clustered into sparse and dense regions. Some “giant” genes were observed that had average coding sequence sizes but were spread over genomic lengths significantly larger than those of their human orthologs. Although three-quarters of predicted human proteins have a strong match toFugu, approximately a quarter of the human proteins had highly diverged from or had no pufferfish homologs, highlighting the extent of protein evolution in the 450 million years since teleosts and mammals diverged. Conserved linkages between Fugu and human genes indicate the preservation of chromosomal segments from the common vertebrate ancestor, but with considerable scrambling of gene order.
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Sequencing Methods
Supplemental Data
Tables S1 to S7
Figs. S1 to S8
References
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Science
Volume 297 | Issue 5585
23 August 2002
23 August 2002
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Received: 21 March 2002
Accepted: 14 June 2002
Published in print: 23 August 2002
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