Abstract
In the S locus-controlled self-incompatibility system of Brassica, recognition of self-related pollen at the surface of stigma epidermal cells leads to inhibition of pollen tube development. The female (stigmatic) determinant of this recognition reaction is a polymorphic transmembrane receptor protein kinase encoded at the S locus. Another highly polymorphic, anther-expressed gene, SCR, also encoded at the S locus, fulfills the requirements for the hypothesized pollen determinant. Loss-of-function and gain-of-function studies prove that the SCR gene product is necessary and sufficient for determining pollen self-incompatibility specificity, possibly by acting as a ligand for the stigmatic receptor.
Publication types
- Research Support, U.S. Gov't, Non-P.H.S.
- Research Support, U.S. Gov't, P.H.S.
MeSH terms
- Alleles
- Amino Acid Sequence
- Brassica / genetics
- Brassica / physiology*
- Cysteine / chemistry
- Genes, Plant*
- Germination
- Glycoproteins / genetics
- Glycoproteins / metabolism
- Haplotypes
- Ligands
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Plant Proteins / chemistry
- Plant Proteins / genetics*
- Plant Proteins / metabolism
- Plant Proteins / physiology*
- Plant Structures / genetics
- Plant Structures / physiology
- Pollen / genetics
- Pollen / physiology*
- Polymorphism, Genetic
- Protein Kinases / genetics
- Protein Kinases / metabolism
- Sequence Alignment
- Transformation, Genetic
Substances
- Glycoproteins
- Ligands
- Plant Proteins
- SCR protein, Brassica oleracea
- self-incompatibility locus specific glycoprotein, Brassica
- Protein Kinases
- S-receptor kinase
- Cysteine
Associated data
- GENBANK/AF195625
- GENBANK/AF195626
- GENBANK/AF195627