Interactions between specific phytoplankton and bacteria affect lake bacterial community succession
Sara F. Paver
Program in Ecology, Evolution, and Conservation Biology, University of Illinois, Urbana, IL, USA
Search for more papers by this authorKevin R. Hayek
School of Integrative Biology, University of Illinois, Urbana, IL, USA
Search for more papers by this authorKelsey A. Gano
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorJennie R. Fagen
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorChristopher T. Brown
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorAustin G. Davis-Richardson
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorDavid B. Crabb
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorRichard Rosario-Passapera
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorAdriana Giongo
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorEric W. Triplett
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorCorresponding Author
Angela D. Kent
Program in Ecology, Evolution, and Conservation Biology, University of Illinois, Urbana, IL, USA
Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana, IL, USA
For correspondence. E-mail [email protected]; Tel. (+1) 217 333 4216; Fax (+1) 217 244 3219.Search for more papers by this authorSara F. Paver
Program in Ecology, Evolution, and Conservation Biology, University of Illinois, Urbana, IL, USA
Search for more papers by this authorKevin R. Hayek
School of Integrative Biology, University of Illinois, Urbana, IL, USA
Search for more papers by this authorKelsey A. Gano
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorJennie R. Fagen
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorChristopher T. Brown
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorAustin G. Davis-Richardson
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorDavid B. Crabb
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorRichard Rosario-Passapera
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorAdriana Giongo
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorEric W. Triplett
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA
Search for more papers by this authorCorresponding Author
Angela D. Kent
Program in Ecology, Evolution, and Conservation Biology, University of Illinois, Urbana, IL, USA
Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana, IL, USA
For correspondence. E-mail [email protected]; Tel. (+1) 217 333 4216; Fax (+1) 217 244 3219.Search for more papers by this authorSummary
Time-series observations and a phytoplankton manipulation experiment were combined to test the hypothesis that phytoplankton succession effects changes in bacterial community composition. Three humic lakes were sampled weekly May–August and correlations between relative abundances of specific phytoplankton and bacterial operational taxonomic units (OTUs) in each time series were determined. To experimentally characterize the influence of phytoplankton, bacteria from each lake were incubated with phytoplankton from one of the three lakes or no phytoplankton. Following incubation, variation in bacterial community composition explained by phytoplankton treatment increased 65%, while the variation explained by bacterial source decreased 64%. Free-living bacteria explained, on average, over 60% of the difference between phytoplankton and corresponding no-phytoplankton control treatments. Fourteen out of the 101 bacterial OTUs that exhibited positively correlated patterns of abundance with specific algal populations in time-series observations were enriched in mesocosms following incubation with phytoplankton, and one out of 59 negatively correlated bacterial OTUs was depleted in phytoplankton treatments. Bacterial genera enriched in mesocosms containing specific phytoplankton assemblages included Limnohabitans (clade betI-A), Bdellovibrio and Mitsuaria. These results suggest that effects of phytoplankton on certain bacterial populations, including bacteria tracking seasonal changes in algal-derived organic matter, result in correlations between algal and bacterial community dynamics.
Supporting Information
| Filename | Description |
|---|---|
| emi12131-sup-0001-si.docx150.9 KB | Fig. S1. Total number of bacteria in mesocosms (average ± standard error) with (+ NP) and without added nitrogen and phosphorus on days 1, 3 and 7 from experiments described by Kent and colleagues (2006) (unpublished data). This experiment was conducted in Crystal Bog using the same mesocosm containers and incubation length used in the current study. The < 1 μm size fraction is analogous to the no-phytoplankton control treatment in this study and < 243 μm size fraction is similar to phytoplankton assemblage treatments. Without added nutrients, bacterial abundance remains relatively stable over the incubation period. Table S1. Initial and final phytoplankton cell counts (cells ml−1 ± standard error) over a 7-day pilot experiment. No phytoplankton cells were detected in initial samples collected from no-phytoplankton control treatments. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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