Abstract
Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure.
Publication types
- Evaluation Study
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
- Bacteria / classification*
- Bacteria / genetics
- Bacteria / isolation & purification
- DNA Primers
- DNA, Bacterial / analysis
- DNA, Fungal / analysis
- Ecosystem*
- Fungi / classification*
- Fungi / genetics
- Fungi / isolation & purification
- Polymerase Chain Reaction / methods*
- Sensitivity and Specificity
- Soil Microbiology*
- Species Specificity
Substances
- DNA Primers
- DNA, Bacterial
- DNA, Fungal