Glyphosate and glyphosate-based herbicide exposure during the peripartum period affects maternal brain plasticity, maternal behaviour and microbiome
Julie Dechartres
Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé Environnement et Travail), UMR_S 1085, Rennes, France
Search for more papers by this authorJodi L. Pawluski
Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé Environnement et Travail), UMR_S 1085, Rennes, France
Search for more papers by this authorMarie-Madeleine Gueguen
Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé Environnement et Travail), UMR_S 1085, Rennes, France
Search for more papers by this authorAmin Jablaoui
Micalis, INRA, AgroParisTech, Univ Paris-Saclay, Jouy-en-Josas, France
Search for more papers by this authorEmmanuelle Maguin
Micalis, INRA, AgroParisTech, Univ Paris-Saclay, Jouy-en-Josas, France
Search for more papers by this authorMoez Rhimi
Micalis, INRA, AgroParisTech, Univ Paris-Saclay, Jouy-en-Josas, France
Search for more papers by this authorCorresponding Author
Thierry D. Charlier
Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé Environnement et Travail), UMR_S 1085, Rennes, France
Correspondence
Thierry D. Charlier, University of Rennes 1, IRSET, 9 Av Prof Leon Bernard, 35000 Rennes, France.
Email: [email protected]
Search for more papers by this authorJulie Dechartres
Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé Environnement et Travail), UMR_S 1085, Rennes, France
Search for more papers by this authorJodi L. Pawluski
Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé Environnement et Travail), UMR_S 1085, Rennes, France
Search for more papers by this authorMarie-Madeleine Gueguen
Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé Environnement et Travail), UMR_S 1085, Rennes, France
Search for more papers by this authorAmin Jablaoui
Micalis, INRA, AgroParisTech, Univ Paris-Saclay, Jouy-en-Josas, France
Search for more papers by this authorEmmanuelle Maguin
Micalis, INRA, AgroParisTech, Univ Paris-Saclay, Jouy-en-Josas, France
Search for more papers by this authorMoez Rhimi
Micalis, INRA, AgroParisTech, Univ Paris-Saclay, Jouy-en-Josas, France
Search for more papers by this authorCorresponding Author
Thierry D. Charlier
Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé Environnement et Travail), UMR_S 1085, Rennes, France
Correspondence
Thierry D. Charlier, University of Rennes 1, IRSET, 9 Av Prof Leon Bernard, 35000 Rennes, France.
Email: [email protected]
Search for more papers by this authorFunding Information
TDC was supported by La Région Bretagne (SAD), Rennes Métropole and the University of Rennes 1 (Défis Emergents 2018). JD is funded from the University of Rennes 1 (Bourse fléchée UR1). Funding sources had no involvement in study design, in the collection, analysis and interpretation of data, in the writing of the report, and in the decision to submit the article for publication.
Abstract
Glyphosate is found in a large array of non-selective herbicides such as Roundup® (Monsanto, Creve Coeur, MO, USA) and is by far the most widely used herbicide. Recent work in rodent models suggests that glyphosate-based herbicides during development can affect neuronal communication and result in altered behaviours, albeit through undefined mechanisms of action. To our knowledge, no study has investigated the effects glyphosate or its formulation in herbicide on maternal behaviour and physiology. In the present study, relatively low doses of glyphosate (5 mg kg-1 d-1), Roundup® (5 mg kg-1 d-1 glyphosate equivalent), or vehicle were administered by ingestion to Sprague-Dawley rats from gestational day (GD) 10 to postpartum day (PD)22. The treatments significantly altered licking behaviour toward pups between PD2 and PD6. We also show in the dams at PD22 that Roundup exposure affected the maturation of doublecortin-immunoreactive new neurones in the dorsal dentate gyrus of the hippocampus of the mother. In addition, the expression of synaptophysin was up-regulated by glyphosate in the dorsal and ventral dentate gyrus and CA3 regions of the hippocampus, and down-regulated in the cingulate gyrus. Although a direct effect of glyphosate alone or its formulation on the central nervous system is currently not clear, we show that gut microbiota is significantly altered by the exposure to the pesticides, with significant alteration of the phyla Bacteroidetes and Firmicutes. This is the first study to provide evidence that glyphosate alone or in formulation (Roundup) differentially affects maternal behaviour and modulates neuroplasticity and gut microbiota in the mother.
CONFLICT OF INTERESTS
The authors declare that they have no conflicts of interest.
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