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Transcriptional profiling of Saccharomyces cerevisiae cells under adhesion-inducing conditions

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Abstract

The ability to adhere to other cells is one of the most prominent determinants of fungal pathogenicity. Thus, adherence of fungi to human tissues or plastics triggers hospital-acquired fungal infections, which are an increasing clinical problem, especially in immunocompromised persons. In the model fungus Saccharomyces cerevisiae adhesion can be induced by starvation for amino acids, and depends on the transcriptional activator of the general amino acid control system, Gcn4p. However, not much is known about the transcriptional program that mediates adhesive growth under such conditions. In this study, we present a genome-wide transcriptional analysis of Σ1278b yeast cells that were subjected to adhesion-inducing conditions imposed by amino acid starvation. Twenty-two novel genes were identified as inducible by amino acid starvation; 72 genes belonging to different functional groups, which were not previously known to be regulated by Gcn4p, require Gcn4p for full transcriptional induction under adhesion-inducing conditions. In addition, several genes were identified in Σ1278b cells that were inducible by amino acid starvation in a Gcn4p-independent manner. Our data suggest that adhesion of yeast cells induced by amino acid starvation is regulated by a complex, Σ1278b-specific transcriptional response.

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Acknowledgements

We thank Drs. Andrea Muffler and Jürgen Bauer from Axaron Bioscience AG and Dr. Paul Kayne from Bristol-Myers Squibb for help with initial microarray experiments. We are grateful to Katrin Bömeke, Dr. Katrin Düvel, Claudia Fischer and Dr. Oliver Valerius for critically reading the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft, the Fonds der Chemischen Industrie, and the Volkswagen Foundation. The experiments presented here comply with the current laws of Germany.

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Authors and Affiliations

  1. Institute of Microbiology and Genetics, Georg-August-University, Grisebachstrasse 8, 37077, Göttingen, Germany

    Malte Kleinschmidt, Olav Grundmann, Hans-Ulrich Mösch & Gerhard H. Braus

  2. Institute for Theoretical Biology, Humboldt-University, Invalidenstrasse 43, 10115, Berlin, Germany

    Nils Blüthgen

  3. Department of Microbiology, Max Planck Institute Bremen, Celsiusstrasse 1, 28359, Bremen, Germany

    Olav Grundmann

  4. Department of Genetics, Philipps-University Marburg, Karl-von-Frisch-Strasse, 35032, Marburg, Germany

    Hans-Ulrich Mösch

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  1. Malte Kleinschmidt
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  2. Olav Grundmann
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  3. Nils Blüthgen
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  4. Hans-Ulrich Mösch
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  5. Gerhard H. Braus
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Correspondence to Gerhard H. Braus.

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Communicated by M. Johnston

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Kleinschmidt, M., Grundmann, O., Blüthgen, N. et al. Transcriptional profiling of Saccharomyces cerevisiae cells under adhesion-inducing conditions. Mol Genet Genomics 273, 382–393 (2005). https://doi.org/10.1007/s00438-005-1139-4

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