Summary
Diabetes mellitus is the most common disease in Westernized countries in large part because of the rising prevalence of obesity and physical inactivity. In addition, diabetes mellitus is an important risk factor for both heart failure and ischemic heart disease. As insulin resistance is known as an important pathophysiological feature in the cardiac diseases, understanding the mechanisms responsible for altered metabolism and insulin signaling in the diabetic heart may help identify novel targets in these conditions. Phosphatidylinositol (PI)-3 kinase (PI3K) and Akt are key signaling molecules in insulin and insulin-like growth factor-1 (IGF-1), which induce multiple biological effects in the heart such as cell survival and hypertrophy. Here, we have shown several fundamental techniques to study the role of PI3K and Akt in heart diseases.
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Acknowledgments
This work was supported in part by NIH HL04250 to T. Matsui and NIH R01 HL66895 and American Diabetes Association Research Award 7-04-RA-23 to A. J. Davidoff. The authors thank Dr. Kenta Hara for his helpful advice.
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Matsui, T., Davidoff, A.J. (2007). Assessment of PI-3 Kinase and Akt in Ischemic Heart Diseases in Diabetes. In: Sreejayan, N., Ren, J. (eds) Vascular Biology Protocols. Methods in Molecular Medicine™, vol 139. Humana Press. https://doi.org/10.1007/978-1-59745-571-8_22
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DOI: https://doi.org/10.1007/978-1-59745-571-8_22
Publisher Name: Humana Press
Print ISBN: 978-1-58829-574-3
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