Abstract
A human cDNA coding for a protein related to the vascular permeability factor (VPF) was isolated from a term placenta cDNA library; we therefore named its product placenta growth factor (PlGF). PlGF is a 149-amino-acid-long protein and is highly homologous (53% identity) to the platelet-derived growth factor-like region of human VPF. Computer analyses reveal a putative signal peptide and two probable N-glycosylation sites in the PlGF protein, one of which is also conserved in human VPF. By using N-glycosidase F, tunicamycin, and specific antibodies produced in both chicken and rabbit, we demonstrate that PlGF, derived from transfected COS-1 cells, is actually N-glycosylated and secreted into the medium. In addition, PlGF, like VPF, proves to be a dimeric protein. Finally, a conditioned medium from COS-1 cells containing PlGF is capable of stimulating specifically the growth of CPA, a line of endothelial cells, in vitro.
Publication types
- Comparative Study
- Research Support, Non-U.S. Gov't
MeSH terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Division / drug effects
- Cell Line
- Choriocarcinoma
- Cloning, Molecular
- DNA / genetics*
- DNA / isolation & purification
- Endothelial Growth Factors / genetics*
- Endothelium, Vascular / cytology
- Endothelium, Vascular / drug effects
- Female
- Gene Library
- Humans
- Lymphokines / genetics*
- Macromolecular Substances
- Molecular Sequence Data
- Molecular Weight
- Oligodeoxyribonucleotides
- Oligonucleotide Probes
- Placenta / physiology*
- Placenta Growth Factor
- Pregnancy
- Pregnancy Proteins / analysis
- Pregnancy Proteins / genetics*
- Pregnancy Proteins / pharmacology
- Recombinant Proteins / analysis
- Recombinant Proteins / pharmacology
- Sequence Homology, Nucleic Acid
- Transfection
- Uterine Neoplasms
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
Substances
- Endothelial Growth Factors
- Lymphokines
- Macromolecular Substances
- Oligodeoxyribonucleotides
- Oligonucleotide Probes
- PGF protein, human
- Pregnancy Proteins
- Recombinant Proteins
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
- Placenta Growth Factor
- DNA
Associated data
- GENBANK/S60069
- GENBANK/S60071
- GENBANK/S60073
- GENBANK/S60076
- GENBANK/S60079
- GENBANK/S60081
- GENBANK/S60988
- GENBANK/S61158
- GENBANK/S61170
- GENBANK/X54936