Detection of Sweet and Umami Taste in the Absence of Taste Receptor T1r3
Abstract
The tastes of sugars (sweet) and glutamate (umami) are thought to be detected by T1r receptors expressed in taste cells. Molecular genetics and heterologous expression implicate T1r2 plus T1r3 as a sweet-responsive receptor,and T1r1 plus T1r3,as well as a truncated form of the type 4 metabotropic glutamate receptor (taste-mGluR4),as umami-responsive receptors. Here,we show that mice lacking T1r3 showed no preference for artificial sweeteners and had diminished but not abolished behavioral and nerve responses to sugars and umami compounds. These results indicate that T1r3-independent sweet- and umami-responsive receptors and/or pathways exist in taste cells.
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Supported by NIHgrants DC 004766 (S.D.), DC 003055 and DC 003155 (R.F.M.), and by a grant from the Program for Promotion of Basic Research Activities for innovative Bioscience from the Bio-oriented Technology Research Advancement Institution (BRAIN) (Y.N.). R.F.M. is an Associate Investigator of the Howard Hughes Medical Institute.
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Science
Volume 301 | Issue 5634
8 August 2003
8 August 2003
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American Association for the Advancement of Science.
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Received: 23 May 2003
Accepted: 9 July 2003
Published in print: 8 August 2003
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- Sami Damak et al.
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