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Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor

Y. Shing, J. Folkman, R. Sullivan, C. Butterfield, J. Murray, and M. KlagsbrunAuthors Info & Affiliations
Science
23 Mar 1984
Vol 223, Issue 4642
pp. 1296-1299
DOI: 10.1126/science.6199844
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Abstract

A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure. The purified growth factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.

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Information & Authors

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Published In

Science
Volume 223 | Issue 4642
23 March 1984

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© 1984.

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Published in print: 23 March 1984

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Authors

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Y. Shing
Departments of Biological Chemistry, Anatomy, and Surgery, Children's Hospital Medical Center, and Harvard Medical School, Boston, Massachusetts 021/5
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J. Folkman
Departments of Biological Chemistry, Anatomy, and Surgery, Children's Hospital Medical Center, and Harvard Medical School, Boston, Massachusetts 021/5
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R. Sullivan
Departments of Biological Chemistry, Anatomy, and Surgery, Children's Hospital Medical Center, and Harvard Medical School, Boston, Massachusetts 021/5
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C. Butterfield
Departments of Biological Chemistry, Anatomy, and Surgery, Children's Hospital Medical Center, and Harvard Medical School, Boston, Massachusetts 021/5
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J. Murray
Departments of Biological Chemistry, Anatomy, and Surgery, Children's Hospital Medical Center, and Harvard Medical School, Boston, Massachusetts 021/5
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M. Klagsbrun
Departments of Biological Chemistry, Anatomy, and Surgery, Children's Hospital Medical Center, and Harvard Medical School, Boston, Massachusetts 021/5
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  • Y. Shing et al.
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Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor.Science223,1296-1299(1984).DOI:10.1126/science.6199844

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