Abstract
The Spike (S) protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) plays important roles in viral pathogenesis and potentially in the development of an effective vaccine against this virulent infectious disease. In this study, the codon-optimized S gene of SARS-CoV was synthesized to construct DNA vaccine plasmids expressing either the full-length or segments of the S protein. High titer S-specific immunoglobulin G antibody responses were elicited in rabbits immunized with DNA against various segments of the S protein. Two neutralizing domains were identified on the S protein, one at the N terminus (Ser12-Thr535) and the other near the C terminus (Arg797-Ile1192).
Publication types
- Research Support, U.S. Gov't, P.H.S.
MeSH terms
- Animals
- Antibodies, Viral / immunology*
- Cell Line
- Chlorocebus aethiops
- Cytopathogenic Effect, Viral
- Epitopes / chemistry*
- Epitopes / immunology
- Humans
- Immunization
- Membrane Glycoproteins / chemistry*
- Membrane Glycoproteins / immunology*
- Membrane Glycoproteins / metabolism
- Neutralization Tests
- Rabbits
- Severe Acute Respiratory Syndrome / immunology
- Severe Acute Respiratory Syndrome / prevention & control
- Severe acute respiratory syndrome-related coronavirus / immunology*
- Spike Glycoprotein, Coronavirus
- Vaccines, DNA / administration & dosage
- Vaccines, DNA / immunology
- Vero Cells
- Viral Envelope Proteins / chemistry*
- Viral Envelope Proteins / immunology*
- Viral Envelope Proteins / metabolism
- Viral Vaccines / administration & dosage
- Viral Vaccines / immunology
Substances
- Antibodies, Viral
- Epitopes
- Membrane Glycoproteins
- Spike Glycoprotein, Coronavirus
- Vaccines, DNA
- Viral Envelope Proteins
- Viral Vaccines
- spike glycoprotein, SARS-CoV
- spike protein, mouse hepatitis virus