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Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor

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Abstract

Media and growth conditions were optimized for the microaerobic cultivation of Magnetospirillum gryphiswaldense in flasks and in a fermentor, resulting in significantly increased cell and magnetosome yields, compared with earlier studies. A reliable method was established for the automatic control of low dissolved oxygen tensions (pO2) in the fermentor (oxystat). Growth and magnetosome formation by M. gryphiswaldense, M. magnetotacticum and Magnetospirillum sp. AMB-1 were studied at various oxygen concentrations. Despite differences in their growth responses with respect to oxygen, we found a clear correlation between pO2 and magnetosome formation in all three Magnetospirillum strains. Magnetite biomineralization was induced only below a threshold value of 20 mbar O2 and optimum conditions for magnetosome formation were found at a pO2 of 0.25 mbar (1 bar = 105 Pa). A maximum yield of 6.3 mg magnetite l-1 day-1 was obtained with M. gryphiswaldense grown under oxystat conditions, which is the highest magnetosome productivity reported so far for a magnetotactic bacterium. In conclusion, the presented results provide the basis for large-scale cultivation of magnetospirilla under defined conditions.

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Acknowledgements

We thank J. Hoffmann (B. Braun Biotech International) for valuable suggestions and helpful discussions concerning the development of the oxystat. We are grateful to K. Grünberg and C. Flies for help with electron microscopy and F. Mayer (Göttingen) for providing access to the EM. The help of A. Baier and O. Menke in several growth experiments is greatly acknowledged. This work was supported by the Max-Planck-Gesellschaft, the Federal Ministry of Education and Research (BMBF), and the Deutsche Forschungsgemeinschaft.

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  1. Max-Planck-Institute for Marine Microbiology, Celsiusstrasse 1, 28359, Bremen, Germany

    U. Heyen & D. Schüler

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  1. U. Heyen
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  2. D. Schüler
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Correspondence to D. Schüler.

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Heyen, U., Schüler, D. Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor. Appl Microbiol Biotechnol 61, 536–544 (2003). https://doi.org/10.1007/s00253-002-1219-x

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  • DOI: https://doi.org/10.1007/s00253-002-1219-x

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