ACS Publications. Most Trusted. Most Cited. Most Read

OR SEARCH CITATIONS

My Activity
Publications
CONTENT TYPES

Figure 1Loading Img
Download Hi-Res ImageDownload to MS-PowerPointCite This:ACS Synth. Biol. 2018, 7, 2, 718-726
RETURN TO ISSUEPREVResearch ArticleNEXT

Genetically Encoded Circuit for Remote Regulation of Cell Migration by Magnetic Fields

View Author Information
† Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada
‡ Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, 10 King’s College Circle, Toronto, Ontario M5S 3G4, Canada
*E-mail: [email protected]. Tel: 416-978-7772. Fax: 416-978-4317.
Cite this: ACS Synth. Biol. 2018, 7, 2, 718–726
Publication Date (Web):January 17, 2018
https://doi.org/10.1021/acssynbio.7b00415
Copyright © 2018 American Chemical Society
Request reuse permissions

    Article Views

    1358

    Altmetric

    -

    Citations

    19
    LEARN ABOUT THESE METRICS
    Other access options
    Get e-Alerts
    Supporting Info (3)»
    SUBJECTS:

    Abstract

    Abstract Image

    Magnetoreception can be generally defined as the ability to transduce the effects of a magnetic field into a cellular response. Magnetic stimulation at the cellular level is particularly attractive due to its ability for deep penetration and minimal invasiveness, allowing remote regulation of engineered biological processes. Previously, a magnetic-responsive genetic circuit was engineered using the transient receptor potential vanilloid 1 (TRPV1) and the iron containing ferritin protein (i.e., the TF circuit). In this study, we combined the TF circuit with a Ca2+ activated RhoA protein (CaRQ) to allow a magnetic field to remotely regulate cell migration. Cells expressing the TF circuit and CaRQ exhibited consistent dynamic protrusions, leading to migration along a porous membrane, directed spreading in response to a magnetic field gradient, as well as wound healing. This work offers a compelling interface for programmable electrical devices to control the migration of living systems for potential applications in cell-based therapy.

    KEYWORDS:

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acssynbio.7b00415.

    • Figure S1 (PDF)

    • Video S1 (AVI)

    • Video S2 (AVI)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 19 publications.

    1. Changyou Chen, Haitao Chen, Pingping Wang, Xue Wang, Xuting Wang, Chuanfang Chen, Weidong Pan. Reactive Oxygen Species Activate a Ferritin-Linked TRPV4 Channel under a Static Magnetic Field. ACS Chemical Biology 2024, 19 (5) , 1151-1160. https://doi.org/10.1021/acschembio.4c00090
    2. Mohammed Nadim Sardoiwala, Ankur Sood, Liku Biswal, Subhasree Roy Choudhury, Surajit Karmakar. Reconstituted Super Paramagnetic Protein “Magnetotransferrin” for Brain Targeting to Attenuate Parkinsonism. ACS Applied Materials & Interfaces 2023, 15 (10) , 12708-12718. https://doi.org/10.1021/acsami.2c20990
    3. Jordan W. Mundell, Matthew I. Brier, Everest Orloff, Sarah A. Stanley, Jonathan S. Dordick. Alternating magnetic fields drive stimulation of gene expression via generation of reactive oxygen species. iScience 2024, 27 (3) , 109186. https://doi.org/10.1016/j.isci.2024.109186
    4. Xiang Wang, Qianyi Liang, Yixuan Luo, Jianwen Ye, Yin Yu, Fei Chen. Engineering the next generation of theranostic biomaterials with synthetic biology. Bioactive Materials 2024, 32 , 514-529. https://doi.org/10.1016/j.bioactmat.2023.10.018
    5. Santiago R. Unda, Lisa E. Pomeranz, Roberta Marongiu, Xiaofei Yu, Leah Kelly, Gholamreza Hassanzadeh, Henrik Molina, George Vaisey, Putianqi Wang, Jonathan P. Dyke, Edward K. Fung, Logan Grosenick, Rick Zirkel, Aldana M. Antoniazzi, Sofya Norman, Conor M. Liston, Chris Schaffer, Nozomi Nishimura, Sarah A. Stanley, Jeffrey M. Friedman, Michael G. Kaplitt. Bidirectional Regulation of Motor Circuits Using Magnetogenetic Gene Therapy. 2023https://doi.org/10.1101/2023.07.13.548699
    6. Xiaofeng Lu, Galong Li, Wangbo Jiao, Kuo Li, Tingbin Zhang, Xiaoli Liu, Haiming Fan. Magnetic nanomaterials‐mediated neuromodulation. WIREs Nanomedicine and Nanobiotechnology 2023, 15 (4) https://doi.org/10.1002/wnan.1890
    7. Lisa Pomeranz, Rosemary Li, Xiaofei Yu, Leah Kelly, Gholamreza Hassanzadeh, Henrik Molina, Daniel Gross, Matthew Brier, George Vaisey, Putianqi Wang, Maria Jimenez-Gonzalez, Adolfo Garcia-Ocana, Jonathan Dordick, Jeffrey Friedman, Sarah Stanley. Magnetogenetic cell activation using endogenous ferritin. 2023https://doi.org/10.1101/2023.06.20.545120
    8. Clare M. Robinson, Nina E. Short, David T. Riglar. Achieving spatially precise diagnosis and therapy in the mammalian gut using synthetic microbial gene circuits. Frontiers in Bioengineering and Biotechnology 2022, 10 https://doi.org/10.3389/fbioe.2022.959441
    9. Maryam Moarefian, Antonia McDonnell Capossela, Ryan Eom, Kiana Aran. Single-Cell Technologies: Advances in Single-Cell Migration and Multi-Omics. GEN Biotechnology 2022, 1 (3) , 246-261. https://doi.org/10.1089/genbio.2022.0014
    10. Maren Laughlin, Bradley Cooke, Kerri Boutelle, Cary R. Savage, Alexxai Kravitz, Dana Small, Zoe Arvanitakis, Alex Martin, Luke E. Stoeckel. Neuroimaging and modulation in obesity and diabetes research: 10th anniversary meeting. International Journal of Obesity 2022, 46 (4) , 718-725. https://doi.org/10.1038/s41366-021-01025-8
    11. Susel Del Sol-Fernández, Pablo Martínez-Vicente, Pilar Gomollón-Zueco, Christian Castro-Hinojosa, Lucía Gutiérrez, Raluca M. Fratila, María Moros. Magnetogenetics: remote activation of cellular functions triggered by magnetic switches. Nanoscale 2022, 14 (6) , 2091-2118. https://doi.org/10.1039/D1NR06303K
    12. Xiuying Li, Hejian Xiong, Nicholas Rommelfanger, Xueqi Xu, Jonghae Youn, Paul A. Slesinger, Guosong Hong, Zhenpeng Qin. Nanotransducers for wireless neuromodulation. Matter 2021, 4 (5) , 1484-1510. https://doi.org/10.1016/j.matt.2021.02.012
    13. Yang Cui, Ying Yang, Dong Qiu. Design of selective cell migration biomaterials and their applications for tissue regeneration. Journal of Materials Science 2021, 56 (6) , 4080-4096. https://doi.org/10.1007/s10853-020-05537-y
    14. Matthew I. Brier, Jordan W. Mundell, Xiaofei Yu, Lichao Su, Alexander Holmann, Jessica Squeri, Baolin Zhang, Sarah A. Stanley, Jeffrey M. Friedman, Jonathan S. Dordick. Uncovering a possible role of reactive oxygen species in magnetogenetics. Scientific Reports 2020, 10 (1) https://doi.org/10.1038/s41598-020-70067-1
    15. Michael A. Wheeler, Christopher D. Deppmann, Manoj K. Patel, Ali D. Güler. Reply to: Magneto is ineffective in controlling electrical properties of cerebellar Purkinje cells, Assessing the utility of Magneto to control neuronal excitability in the somatosensory cortex and Revaluation of magnetic properties of Magneto. Nature Neuroscience 2020, 23 (9) , 1051-1054. https://doi.org/10.1038/s41593-019-0472-6
    16. Matthew I. Brier, Jonathan S. Dordick. Remote activation of cellular signaling. Science 2020, 368 (6494) , 936-937. https://doi.org/10.1126/science.abb9122
    17. Miriam Hernández-Morales, Trisha Shang, Jingjia Chen, Victor Han, Chunlei Liu. Lipid Oxidation Induced by RF Waves and Mediated by Ferritin Iron Causes Activation of Ferritin-Tagged Ion Channels. Cell Reports 2020, 30 (10) , 3250-3260.e7. https://doi.org/10.1016/j.celrep.2020.02.070
    18. Mladen Barbic. Possible magneto-mechanical and magneto-thermal mechanisms of ion channel activation in magnetogenetics. eLife 2019, 8 https://doi.org/10.7554/eLife.45807
    19. Sarah A. Stanley, Jeffrey M. Friedman. Electromagnetic Regulation of Cell Activity. Cold Spring Harbor Perspectives in Medicine 2019, 9 (5) , a034322. https://doi.org/10.1101/cshperspect.a034322
    Download PDF

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect