This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
Cryo-EM structure of cadmium-bound human ABCB6
Communications Biology Open Access 31 May 2024
Human CD4-binding site antibody elicited by polyvalent DNA prime-protein boost vaccine neutralizes cross-clade tier-2-HIV strains
Nature Communications Open Access 21 May 2024
Interplay between Mg2+ and Ca2+ at multiple sites of the ryanodine receptor
Nature Communications Open Access 15 May 2024
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Henderson, R. et al. Structure 20, 205–214 (2012).
Saxton, W.O. & Baumeister, W. J. Microsc. 127, 127–138 (1982).
Stewart, A. & Grigorieff, N. Ultramicroscopy 102, 67–84 (2004).
Scheres, S.H. J. Mol. Biol. 415, 406–418 (2012).
Henderson, R. et al. J. Mol. Biol. 413, 1028–1046 (2011).
Scheres, S.H. et al. Nat. Protoc. 3, 977–990 (2008).
Böttcher, B., Wynne, S.A. & Crowther, R.A. Nature 386, 88–91 (1997).
Rosenthal, P.B. & Henderson, R. J. Mol. Biol. 333, 721–745 (2003).
Acknowledgements
We are grateful to T. Crowther and R. Henderson for helpful discussions and to J. Grimmett for help with computing. T. Crowther provided hepatitis B data, and the National Center for Macromolecular Imaging, which is funded by US National Institutes of Health grant P41RR02250, provided GroEL data. This work was funded by the UK Medical Research Council through grant MC_UP_A025_1013 to S.H.W.S.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Supplementary Text and Figures
Supplementary Figures 1–4, Supplementary Table 1, Supplementary Methods and Supplementary Software (PDF 765 kb)
Rights and permissions
About this article
Cite this article
Scheres, S., Chen, S. Prevention of overfitting in cryo-EM structure determination. Nat Methods 9, 853–854 (2012). https://doi.org/10.1038/nmeth.2115
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.2115
This article is cited by
Cryo-EM structure of cadmium-bound human ABCB6
Communications Biology (2024)
The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA.2.86
Nature Communications (2024)
Self Fourier shell correlation: properties and application to cryo-ET
Communications Biology (2024)
Impact of distinct FG nucleoporin repeats on Nup98 self-association
Nature Communications (2024)
Interplay between Mg2+ and Ca2+ at multiple sites of the ryanodine receptor
Nature Communications (2024)