Evaluation of the roles of four Candida albicans genes in virulence by using gene disruption strains that express URA3 from the native locus

Infect Immun. 2003 Oct;71(10):6101-3. doi: 10.1128/IAI.71.10.6101-6103.2003.

Abstract

Reintroducing URA3 to its native locus in Candida albicans not5, not3, bur2, and kel1 disruption mutants enabled us to directly compare strains with control strain CAI-12. We showed that URA3 position affected orotidine 5'-monophosphate decarboxylase activity, hyphal morphogenesis, adherence, and mortality in murine disseminated candidiasis. After URA3 was reintroduced to its native locus, only NOT5 could be conclusively ascribed a role in virulence.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Candida albicans / genetics*
  • Candida albicans / pathogenicity*
  • Candidiasis / microbiology
  • Cell Adhesion
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Humans
  • In Vitro Techniques
  • Mice
  • Mutation
  • Phenotype
  • Virulence / genetics

Substances

  • Fungal Proteins