Resolvex^®

Solid phase extraction consumables

Tecan's suite of microparticulate solid phase extraction (SPE) consumables uses microparticulate sorbents with optimized small flow paths, to ensure greater contact with the analyte molecules. This enables better clean-up of the sample matrix, leading to cleaner sample extracts.

Tecan also offers a variety of sorbent chemistries, from state-of-the-art polymer technologies to unique target resins for more complex workflows.

The difference between using 8 μm sorbent and conventional 40 μm sorbents of surface-matched chemistries was demonstrated by performing a loading experiment in an online cartridge format, measuring the breakthrough of a UV-absorbing analyte from each cartridge using a UV detector (Figure 3).

1. Initially, the cartridge is bypassed; the detector signal reflects the level of analyte in the loading solvent.
2. When the cartridge is placed into the sample stream, the absorbance initially drops and then recovers as analyte breakthrough occurs.
3. The 8 µm cartridge captures roughly four times the analyte quantity compared to the 40 μm cartridge before breakthrough occurs, reaching total saturation immediately afterwards, as shown by the rapid recovery of the detector signal to initial conditions.
4. In contrast, the 40 μm cartridge never reaches saturation, even after a significant period of time.

Figure 3: Loading experiment

Loading time: time required to bind analyte to column material

The high capture efficiency of microparticle sorbents allows much smaller bed sizes to be used in Cerex columns compared to conventional sorbents; a 20 mg Cerex column typically exhibits the same effective capacity as a 100 mg, 40 μm conventional SPE product.

The use of much smaller sorbent beds allows for the use of smaller analyte elution volumes too, as demonstrated in Figure 4.

Figure 4:
Efficient elution with microparticulate sorbents

Curtesy of Spark Holland

Upper bed provides a barrier to liquid flow and filtration during pressure processing steps:

• Upper chamber can be used as a reaction vessel
• Liquid is retained until 15 psi of positive pressure is applied

1. Air lock between the solid barriers prevents the flow of liquid from the upper chamber
2. Monodisperse microparticulate bed with selected chemistry
3. Proprietary ‘Inactive Chemistry Bed’ minimizes interaction of target analytes

NBE microparticulate columns deliver faster flow rates, lower elution volumes and reduced solvent consumption compared to classical SPE and supported liquid extraction (SLE).

• New dual chamber design for high SPE performance
• Microparticulate sorbent for high efficiency
• Integration of pre-SPE steps in the reaction vessel
• Filter for matrix clean-up
• Air lock prevents dripping
• Choice of sorbents for SPE or filtration

Benefits with our Steroid Panel LC-MS kit.

LC-MS/MS is considered the gold standard in the determination of steroid hormones due to its high sensitivity!

• Steroid kit, CE IVDD
• 18 steroids
• 2 chromatographic runs (guarantees the best possible performance of all analytes)
• Only one sample preparation with 96 well SPE plate
• All standards in one mix
• Individual isotopic standard for each analyte
• Total runtime (LC MS): 10 min for 15 steroids, second injection for another 3 analytes in 6 min runtime

LC column is not a part of the Steroid panel LC-MS, available additionally, distributed by Tecan.

Are you looking for a fast and automated solution for sample preparation? We can help!

Automation saves time and resources. By adapting the workflow, an implementation on devices such as Resolvex A200 can be easily implemented.

Applications

• Analyte clean-up and enrichment
• Life science research – protein desalting/digest clean-up, etc.

• Food safety testing
• Environmental monitoring

Resolvex Prep: Automated sample preparation for analytical laboratories.

Introducing Resolvex Prep, the ideal solution for low throughput to medium sample preparation needs in chromatography and mass spectrometry laboratories.

Say goodbye to manual inefficiencies and errors. Resolvex Prep streamlines the process, preparing samples for multiple instruments and delivering them directly into sealed vials for analysis.