Using radiotelemetry, this study aimed to evaluate the influence of tobacco smoke on heart rate (H), body temperature (T) and locomotor activity (A) daily rhythms in rats. The tobacco smoke intoxication was produced with a smoking apparatus. H, T, and A data were captured by radiotelemetry. The study was divided into three periods: a 1-week control period (P1), a 1-week stress period (P2), in order to evaluate the stress induced by the animals' restraint in the smoking apparatus, and a 1-week daily tobacco smoke intoxication period (P3). For P1, P2, and P3, a power spectrum analysis was applied in order to determine the dominant period of rhythmicity. Then, characteristics of the rhythms were determined by cosinor analysis. Statistical comparisons were done by ANOVA. Power spectrum analysis showed that neither stress nor tobacco suppressed the daily rhythmicity. Cosinor revealed some modifications: H amplitude was decreased during P2 and P3 with a greater reduction during P3, while T and A amplitudes were decreased during P2 and P3 without difference between P2 and P3. T acrophase was delayed during P2, while A acrophase was delayed during P2 and P3 without any difference between P2 and P3. These perturbations may reflect the effects of stress and tobacco on the suprachiasmatic nucleus by a dopaminergic mechanism.