Abstract
Peptide binding and lymph node T cell activation studies have been used to characterize T cell recognition of an encephalitogenic T cell autoantigen from myelin basic protein in (PL/J x SJL)F1 mice. Amino acids that determine interactions with either the restriction element of the major histocompatibility complex (MHC) or the encephalitogenic T cell receptor are defined. This information enables the design of peptides that bind MHC yet do not cross-react with the autoantigen. A peptide analog of the encephalitogenic epitope is shown to be "heteroclitic" for MHC binding and activation of encephalitogenic T cells in vitro. This analog is not immunogenic for encephalitogenic T cells in vivo and is shown to inhibit disease that is induced by the autoantigen itself.
Publication types
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, P.H.S.
MeSH terms
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Autoantigens / immunology*
- Chromatography, Affinity
- Encephalomyelitis, Autoimmune, Experimental / immunology*
- Encephalomyelitis, Autoimmune, Experimental / therapy
- Epitopes / immunology
- Histocompatibility Antigens Class II / immunology*
- Histocompatibility Antigens Class II / isolation & purification
- Hybridomas / immunology
- Immunotherapy*
- Lymph Nodes / immunology
- Lymphocyte Activation*
- Mice
- Mice, Inbred Strains
- Molecular Sequence Data
- Oligopeptides / chemical synthesis
- Oligopeptides / immunology*
- T-Lymphocytes / immunology*
Substances
- Antibodies, Monoclonal
- Autoantigens
- Epitopes
- Histocompatibility Antigens Class II
- Oligopeptides