Abstract
A hippocampal 38 kd autoantigen recognized by an autoantibody from the serum of a patient with paraneoplastic limbic encephalitis (PLE) and small cell lung carcinoma (SCLC) was isolated by screening a human hippocampal cDNA library. The 1,991-nucleotide ple21 clone was obtained and the deduced 350-residue protein encoded by the ple21 cDNA clone was found to be highly homologous to the neuron-specific RNA recognition motifs (RRMs)-containing proteins. The homologies were confined to the RRMs and the RRM connecting region. The presence of RRM in the antigenic protein may be important in the pathogenesis of SCLC-associated paraneoplastic neurologic syndrome.
Publication types
- Case Reports
- Comparative Study
- Research Support, Non-U.S. Gov't
MeSH terms
- Amino Acid Sequence
- Autoantigens / biosynthesis*
- Base Sequence
- Carcinoma, Small Cell / metabolism*
- Cerebellum / metabolism
- Cloning, Molecular
- DNA, Complementary / metabolism
- ELAV Proteins
- ELAV-Like Protein 3
- Frontal Lobe / metabolism
- Ganglia, Spinal / metabolism
- Gene Library
- Hippocampus / metabolism*
- Humans
- Lung Neoplasms / metabolism*
- Male
- Middle Aged
- Molecular Sequence Data
- Nerve Tissue Proteins / biosynthesis*
- Paraneoplastic Syndromes / metabolism*
- Poly A / isolation & purification
- Poly A / metabolism
- RNA / isolation & purification
- RNA / metabolism
- RNA, Messenger
- RNA-Binding Proteins*
- Recombinant Proteins / biosynthesis
- Sequence Homology, Amino Acid
Substances
- Autoantigens
- DNA, Complementary
- ELAV Proteins
- ELAV-Like Protein 3
- ELAVL3 protein, human
- Nerve Tissue Proteins
- RNA, Messenger
- RNA-Binding Proteins
- Recombinant Proteins
- Poly A
- RNA