Highlights
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SARS-CoV-2 antigen-specific CD4+CD154+ Th1 cells secrete IL-2 and IFN-γ
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T cell-mediated IL-2 and IFNγ correlate with SARS-CoV-2 IgG humoral immunity
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Naive subjects reach their peak of immunity after the second vaccine dose
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COVID-19 recovered individuals reach their peak of immunity after the first vaccine dose
Summary
Graphical abstract
Keywords
Introduction
- Samanovic M.I.
- Cornelius A.R.
- Wilson J.P.
- Karmacharya T.
- Gray-Gaillard S.L.
- Allen J.R.
- Hyman S.W.
- Moritz G.
- Ali M.
- Koralov S.B.
- et al.
Results
Naive | COVID-19 recovered | ||
---|---|---|---|
No. of individuals | 47 | 45 | |
Age (mean, range) | 39.9, 20–62 | 44.3, 20–76 | |
Female (%) | 78% | 76% | |
Months after infection (%) | N/A | 6–9 (39%), 3–6 (26%), 1–3 (35%) | |
IFN-γ (median, pg/mL) | Pre-vaccination | 1.0 (n = 20) | 46.9 (n = 21) |
dose 1, 10 days (10 days) | 109.5 (n = 20) | 547.8 (n = 21) | |
dose 1, 20 days (20 days) | 52.9 (n = 23) | 351.3 (n = 21) | |
dose 2, 10 days (30 days) | 211.8 (n = 20) | 212 (n = 21) | |
dose 2, 20 days (40 days) | 87.0 (n = 23) | 136.1 (n = 23) | |
IL-2 (median, pg/mL) | Pre-vaccination | 0.5 (n = 20) | 56.9 (n = 21) |
dose 1, 10 days (10 days) | 15.8 (n = 16) | 76.9 (n = 21) | |
dose 1, 20 days (20 days) | 127.0 (n = 22) | 223.5 (n = 20) | |
dose 2, 10 days (30 days) | 187.0 (n = 16) | 148.5 (n = 18) | |
dose 2, 20 days (40 days) | 121.5 (n = 24) | 86.4 (n = 23) | |
SARS-CoV-2 spike-specific IgG (arbitrary units [AU]/mL) | Pre-vaccination | 0.3 (n = 19) | 30.2 (n = 20) |
dose 1, 10 days (10 days) | 4.9 (n = 16) | 332.0 (n = 21) | |
dose 1, 20 days (20 days) | 58.9 (n = 18) | 262.8 (n = 20) | |
dose 2, 10 days (30 days) | 284.4 (n = 16) | 402.4 (n = 20) | |
dose 2, 20 days (40 days) | 269.7 (n = 21) | 291.5 (n = 23) |
- Tauzin A.
- Nayrac M.
- Benlarbi M.
- Gong S.Y.
- Gasser R.
- Beaudoin-Bussieres G.
- Brassard N.
- Laumaea A.
- Vezina D.
- Prevost J.
- et al.
- Painter M.M.
- Mathew D.
- Goel R.R.
- Apostolidis S.A.
- Pattekar A.
- Kuthuru O.
- Baxter A.E.
- Herati R.S.
- Oldridge D.A.
- Gouma S.
- et al.
Discussion
- Tauzin A.
- Nayrac M.
- Benlarbi M.
- Gong S.Y.
- Gasser R.
- Beaudoin-Bussieres G.
- Brassard N.
- Laumaea A.
- Vezina D.
- Prevost J.
- et al.
- Reynolds C.J.
- Pade C.
- Gibbons J.M.
- Butler D.K.
- Otter A.D.
- Menacho K.
- Fontana M.
- Smit A.
- Sackville-West J.E.
- Cutino-Moguel T.
- et al.
Prior SARS-CoV-2 infection rescues B and T cell responses to variants after first vaccine dose.
- Grupper A.
- Rabinowich L.
- Schwartz D.
- Schwartz I.F.
- Ben-Yehoyada M.
- Shashar M.
- Katchman E.
- Halperin T.
- Turner D.
- Goykhman Y.
- et al.
- Kamar N.
- Abravanel F.
- Marion O.
- Couat C.
- Izopet J.
- Del Bello A.
STAR★Methods
Key resources table
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
BV421 anti-human CD196 (CCR6, clone 11A9) | BD Horizon | 562515 |
efluor450 anti-human IL17A (clone eBio64DEC17) | Invitrogen | 48-7179-42 |
BV480 anti-human CD127 (clone HIL-7R-M2) | BD Horizon | 566101 |
efluor506 anti-human CD14 (clone 61D3) | Invitrogen | 69-0149-42 |
BV570 anti-human CD45RA (clone HI100) | BioLegend | 304132 |
BV605 anti-human CD16 (clone 3G8) | BioLegend | 302040 |
BV650 anti-human TNF-alpha (clone Mab11) | BioLegend | 502938 |
Qdot655 anti-human CD27 (clone CLB-27/1) | Invitrogen | Q10066 |
BV711 anti-human OX40 (clone ACT35) | BioLegend | 350030 |
BV750 anti-human CD3 (clone SK7) | BioLegend | 344846 |
BV785 anti-human CCR7 (clone G043H7) | BioLegend | 353230 |
FITC anti-human CD154 (clone 24-31) | Invitrogen | 11-1548-42 |
AF532 anti-human CD4 (clone RPA-T4) | Invitrogen | 58-0049-42 |
PercP anti-human HLA-DR (clone L243) | BioLegend | 307628 |
PercP-Cy5.5 anti-human CCR4 (clone 1G1) | BD PharMingen | 560726 |
PercP-efluor710 anti-human CD8 (clone SK1) | Invitrogen | 46-0087-42 |
PE anti-human IL2 (clone MQ1-17H12) | Invitrogen | 12-7029-42 |
PE-efluor610 anti-human CXCR3 (clone CEW33D) | Invitrogen | 61-1839-42 |
PE-Cy5 anti-human CD137 (clone 4B4-1) | BioLegend | 309808 |
PE-Cy7 anti-human CD69 (clone FN50) | BioLegend | 310912 |
APC anti-human IL4 (clone MP4-25D2) | BioLegend | 500812 |
AF647 anti-human CXCR5 (clone RF8B2) | BD PharMingen | 558113 |
AF700 anti-human IFN-gamma (clone B27) | BD PharMingen | 557995 |
APC-efluor780 anti-human CD25 (clone BC96) | Invitrogen | 47-0259-42 |
APC-Fire810 anti-human CD19 (clone HIB19) | BioLegend | 302272 |
Biological samples | ||
Peripheral blood mononuclear cells (PBMCs) | Patients and healthy donors | N/A |
Specific antibodies measured in serum samples | Patients and healthy donors | N/A |
IFN-gamma measured in whole blood samples | Patients and healthy donors | N/A |
Chemicals, peptides, and recombinant proteins | ||
Zombie NIR Fixable Viability Kit | BioLegend | 423106 |
Ficoll-Paque Plus | GE Healthcare | GE17-1440-02 |
AIM-V medium | GIBCO | 12055091 |
Human AB serum | Gemini Bio-Products, Inc. | 100-512 |
GolgiPlug (Protein Transport Inhibitor) | BD Biosciences | 555029 |
SARS-CoV-2 peptide pools of 15-mers (55 peptides) |
Le Bert et al., 2021
|
N/A |
PEPTIVATOR SARS-COV-2 PROT_S+, RG 60NMOL | MILTENY | 130-127-312 |
PEPTIVATOR SARS-COV-2 PROT_S, RG 60 NMOL | MILTENY | 130-126-701 |
PEPIVATOR SARS-COV-2 PROT_S1,RG 60 NMOL | MILTENY | 130-127-048 |
Critical commercial assays | ||
Access SARS-CoV-2 IgG Antibody Test | Beckman Coulter Inc | C58961 |
Simple Plex Human IL-2 Cartridge | ProteinSimple | SPCKB-PS-000295 |
Simple Plex Human IFN-gamma (3rd Gen) Cartridge | ProteinSimple | SPCKB-PS-002574 |
Deposited data | ||
Raw and analyzed data | This paper | N/A |
Software and algorithms | ||
Flow data were collected with SpectroFlo® Software | Cytek Biosciences | N/A |
Flow cytometry data were analyzed on Cytobank | https://cytobank.org/ | N/A |
Statistical analyses using Graphpad Prism (v9) | Graphpad Holdings, LLC | N/A |
Resource availability
Lead contact
Materials availability
Experimental model and subject details
Method details
SARS-CoV-2 Peptide pools
Whole-blood culture with SARS-CoV-2 peptide pools
Cytokine quantification and analysis
Spike-specific IgG quantification
Flow cytometry
Quantification and statistical analysis
Data and code availability
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All data reported in this paper will be shared by the lead contact upon request.
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This paper does not report original code.
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Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.
Acknowledgments
Author contributions
Declaration of interests
Inclusion and diversity
Supplemental information
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Document S1. Figures S1 and S2
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