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Series GSE31773 Query DataSets for GSE31773
Status Public on Sep 01, 2011
Title Comparison of mRNA expression in circulating T-cells from patients with severe asthma
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Comparison of mRNA expression showed widespread changes in the circulating CD8+ but not CD4+ T-cells from patients with severe asthma. No changes were observed in the CD4+ and CD8+ T-cells in non-severe asthmatics versus healthy controls. Bioinformatics analysis showed that the changes in CD8+ T-cell mRNA expression were associated with multiple pathways involved in T-cell activation. As with mRNAs, we also observed widespread changes in expression of non-coding RNA species including natural antisense, pseudogenes, intronic long ncRNAs and long intergenic long ncRNAs in CD8+ T-cells from severe asthmatics. Measurement of the miRNA expression profile showed selective down-regulation of miR-28-5p in CD8+ T-cells and reduction of miR-146a and miR-146b in both CD4+ and CD8+ T-cells.
 
Overall design mRNA samples were collected from circulating CD4+ and CD8+ T-cells from healthy donors as well as donors with severe and non-severe asthma.
 
Contributor(s) Tsitsiou E, Williams AE, Moschos SA, Patel KA, Rossios C, Jiang X, Adams OD, Macedo P, Booton R, Gibeon D, Chung KF, Lindsay MA
Citation(s) 21917308
Submission date Aug 31, 2011
Last update date Mar 25, 2019
Contact name Ketan Anilkumar Patel
Organization name Pfizer
Department WWRD
Lab Computational Sciences CoE
Street address Ramsgate Road
City Sandwich
ZIP/Postal code CT13 9NJ
Country United Kingdom
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (40)
GSM788299 CD4+_Tcells_non_severe_asthmatic_1
GSM788300 CD4+_Tcells_non_severe_asthmatic_2
GSM788301 CD4+_Tcells_non_severe_asthmatic_3
Relations
BioProject PRJNA145275

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31773_RAW.tar 166.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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