The production of 2D/2D heterostructures (HTs) with favorable electrochemical features is challenging, particularly for semiconductor transition metal dichalcogenides (TMDs). In this studies, we introduce a CO₂ laser plotter-based technology for the realization of HT films comprising reduced graphene oxide (rGO) and 2D-TMDs (MoS₂, WS₂, MoSe₂, and WSe₂) produced via water phase exfoliation. The strategy relies on the Laser-Induced production of HeterosTructures (LIHTs), where after irradiation the nanomaterials exhibit changes in the morphological and chemical structure, becoming conductive easily transferable nanostructured films. The LIHTs were characterized in detail by SEM, XPS, Raman and electrochemical analysis. The laser treatment induces the conversion of GO into conductive highly exfoliated rGO decorated with homogeneously distributed small TMD/TM-oxide nanoflakes. The freestanding LIHT films obtained were employed to build self-contained sensors onto nitrocellulose, where the HT works both as a transducer and sensing surface. The proposed nitrocellulose-sensor manufacturing process is semi-automated and reproducible, multiple HT films may be produced in the same laser treatment and the stencil-printing allows customizable design. Excellent performance in the electroanalytical detection of different molecules such as dopamine (a neurotransmitter), catechin (a flavonol), and hydrogen peroxide was demonstrated, obtaining nanomolar limits of detection and satisfactory recovery rates in biological and agrifood samples, together with high fouling resistance. Considering the robust and rapid laser-induced production of HTs and the versatility of scribing desired patterns, the proposed approach appears as a disruptive technology for the development of electrochemical devices through sustainable and accessible strategies.