Abstract
Current techniques for making transgenic mice are cumbersome, requiring trained personnel, costly infrastructure and collection of many zygotes from mice that are then killed. We developed a reproducible nonterminal technique for transfecting genes in undifferentiated spermatogonia through in vivo electroporation of the testis; about 94% of male mice electroporated with different transgenes successfully sired transgenic pups. Such electroporated males provide a valuable resource for continuous production of transgenic founders for more than a year.
Publication types
- Research Support, Non-U.S. Gov't
MeSH terms
- Animals
- Cell Differentiation
- Electroporation
- Female
- Gene Expression
- Green Fluorescent Proteins / genetics
- Male
- Mice
- Mice, Transgenic / genetics*
- Polymerase Chain Reaction
- Pregnancy
- RNA, Messenger / genetics
- RNA, Messenger / metabolism
- Recombinant Proteins / genetics
- Spermatogonia / cytology*
- Spermatogonia / metabolism
- Testis / cytology
- Testis / metabolism
- Transfection / methods*
Substances
- RNA, Messenger
- Recombinant Proteins
- enhanced green fluorescent protein
- Green Fluorescent Proteins