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Nattokinase: production and application

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Abstract

Nattokinase (NK, also known as subtilisin NAT) (EC 3.4.21.62) is one of the most considerable extracellular enzymes produced by Bacillus subtilis natto. The main interest about this enzyme is due to its direct fibrinolytic activity. Being stable enough in the gastrointestinal tract makes this enzyme a useful agent for the oral thrombolytic therapy. Thus, NK is regarded as a valuable dietary supplement or nutraceutical. Proven safety and ease of mass production are other advantages of this enzyme. In addition to these valuable advantages, there are other applications attributed to NK including treatment of hypertension, Alzheimer’s disease, and vitreoretinal disorders. This review tends to bring a brief description about this valuable enzyme and summarizes the various biotechnological approaches used in its production, recovery, and purification. Some of the most important applications of NK, as well as its future prospects, are also discussed.

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  1. Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Fatemeh Dabbagh, Manica Negahdaripour, Fatemeh Mohammadi, Mozhdeh Zamani & Younes Ghasemi

  2. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

    Fatemeh Dabbagh, Fatemeh Mohammadi, Mozhdeh Zamani & Younes Ghasemi

  3. School of Engineering, Faculty of Science and Engineering, The University of Waikato, Hamilton, New Zealand

    Aydin Berenjian

  4. Department of Food Science and Medical Hydrology, School of Pharmacy, Research Center for Safety Assessment, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Abdolazim Behfar

  5. Department of Resource Development and Management, Shiraz University of Medical Sciences, Shiraz, Iran

    Cambyz Irajie

Authors
  1. Fatemeh Dabbagh
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  2. Manica Negahdaripour
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  3. Aydin Berenjian
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  4. Abdolazim Behfar
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  5. Fatemeh Mohammadi
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  6. Mozhdeh Zamani
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  7. Cambyz Irajie
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  8. Younes Ghasemi
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Corresponding authors

Correspondence to Aydin Berenjian or Younes Ghasemi.

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Dabbagh, F., Negahdaripour, M., Berenjian, A. et al. Nattokinase: production and application. Appl Microbiol Biotechnol 98, 9199–9206 (2014). https://doi.org/10.1007/s00253-014-6135-3

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  • DOI: https://doi.org/10.1007/s00253-014-6135-3

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