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Two tandem promoters to increase gene expression in Lactococcus lactis

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Abstract

Two plasmids, pPAH and pAH, containing a staphylokinase variant gene (sakXH) under the control of two tandem promoters (P32-PlacA) or promoter PlacA alone were constructed and introduced into Lactococcus lactis MG5267. The expression of sakXH in the strain MG5267(pPAH) was approximately twice as high as that in the strain MG5267(pAH), according to the formation of fibrinolytic halos on fibrinolytic plates detected at the same conditions, indicating that the two tandem promoters were stronger than one alone. Difference between the expressions of sakXH under the inducible and non-inducible conditions suggested that PlacA retained its feature as an inducible promoter when fused to promoter P32.

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Authors and Affiliations

  1. Department of Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, P.R. China

    Wenzhong Wei, Hua Xiang & Huarong Tan

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  1. Wenzhong Wei
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  2. Hua Xiang
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  3. Huarong Tan
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Wei, W., Xiang, H. & Tan, H. Two tandem promoters to increase gene expression in Lactococcus lactis . Biotechnology Letters 24, 1669–1672 (2002). https://doi.org/10.1023/A:1020653417455

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