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Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis

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Abstract

Bacillus subtilis was cultivated to high cell density for nattokinase production by pH-stat fed-batch culture. A concentrated mixture solution of glucose and peptone was automatically added by acid-supplying pump when culture pH rose above high limit. Effect of the ratio of glucose to peptone in feeding solution was investigated on cell growth and nattokinase production by changing the ratio from 0.2 to 5 g glucose/g peptone. The highest cell concentration was 77 g/L when the ratio was 0.2 g glucose/g peptone. Cell concentration decreased with increasing the ratio of glucose to peptone in feeding solution, while the optimum condition existed for nattokinase production. The highest nattokinase activity was 14,500 unit/mL at a ratio of 0.33 g glucose/g peptone, which was 4.3 times higher than that in batch culture.

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Acknowledgments

This research was financially supported by the Ministry of Knowledge Economy (MKE) and Korea Institute for Advancement in Technology (KIAT) through the Workforce Development Program in Strategic Technology.

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Authors and Affiliations

  1. Department of Chemical Engineering, Chungbuk National University, Cheongju, 361-763, Chungbuk, Korea

    Eun-Yeong Kwon & Beom Soo Kim

  2. DMJ Biotech Corp, Yeongi, 339-824, Chungnam, Korea

    Kyung Mi Kim, Mi Kyoung Kim & In Young Lee

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  1. Eun-Yeong Kwon
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  2. Kyung Mi Kim
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  3. Mi Kyoung Kim
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  4. In Young Lee
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  5. Beom Soo Kim
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Correspondence to Beom Soo Kim.

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Kwon, EY., Kim, K.M., Kim, M.K. et al. Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis . Bioprocess Biosyst Eng 34, 789–793 (2011). https://doi.org/10.1007/s00449-011-0527-x

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  • DOI: https://doi.org/10.1007/s00449-011-0527-x

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