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GEO help: Mouse over screen elements for information.
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| Status |
Public on May 30, 2009 |
| Title |
P13 cortex RNA |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To estimate transcripts levels in P13 mouse neocortex.
MicroRNAs (miRNAs) play critical roles in the regulation of gene expression. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. We used HITS-CLIP to covalently crosslink native Argonaute (Ago) protein-RNA complexes in mouse brain. This produced two simultaneous datasets—Ago-miRNA and Ago-mRNA binding sites—that were combined with bioinformatic analysis to identify miRNA-target mRNA interaction sites. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Here we include the expression data obtained from dissected P13 mouse neocortex. These data are used for in silico CLIP simulation for normalization of Ago HITS-CLIP tags and also for selecting transcripts expressed in P13 mouse cortex.
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| Overall design |
RNA from the neocortex of 3 wild type P13 CD1 mice. One array per biological replicate.
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| Contributor(s) |
Chi SW, Darnell RB |
| Citation(s) |
19536157 |
| Submission date |
May 28, 2009 |
| Last update date |
Mar 06, 2018 |
| Contact name |
Sung Wook Chi |
| E-mail(s) |
schi@rockefeller.edu
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| Organization name |
Rockefeller Univ
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| Street address |
1230 York Ave,
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL6096 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA115269 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16338_RAW.tar |
70.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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