Abstract
Primary effusion lymphoma (PEL) is a distinct type of lymphoma associated with Kaposi's sarcoma-associated herpesvirus (KSHV) infection. To determine the factors responsible for the unrestrained proliferation of PEL, we have studied the growth factor requirements of the PEL-derived BCBL-1 and BC-1 cell lines. Both cell lines were found to be autocrine growth factor dependent and to release human interleukin-6 (IL-6), viral IL-6 (vIL-6), and human IL-10 in the culture supernatant. To establish whether these cytokines contribute to autocrine growth, neutralizing antibodies against human IL-6, vIL-6, human IL-10, and soluble IL-10 receptor were used. These experiments showed that human IL-10 and, to a lesser degree, vIL-6 serve as autocrine growth factors for BCBL-1 and BC-1 cells. Thus, human IL-10 and vIL-6 are growth factors released and used by PEL cells for autonomous proliferation and may be critical to the development and progression of PEL.
MeSH terms
- Antibodies, Monoclonal / pharmacology
- Autocrine Communication / drug effects
- Autocrine Communication / physiology*
- Cell Division
- Culture Media, Conditioned / pharmacology
- Herpesvirus 8, Human / physiology*
- Humans
- Immune Sera
- Interleukin-10 / antagonists & inhibitors
- Interleukin-10 / immunology
- Interleukin-10 / metabolism
- Interleukin-10 / physiology*
- Interleukin-6 / antagonists & inhibitors
- Interleukin-6 / immunology
- Interleukin-6 / metabolism
- Interleukin-6 / physiology*
- Lymphoma, B-Cell / pathology*
- Lymphoma, B-Cell / virology
- Receptors, Interleukin / antagonists & inhibitors
- Receptors, Interleukin / immunology
- Receptors, Interleukin-10
- Tumor Cells, Cultured
- Viral Proteins / antagonists & inhibitors
- Viral Proteins / immunology
- Viral Proteins / metabolism
- Viral Proteins / physiology*
Substances
- Antibodies, Monoclonal
- Culture Media, Conditioned
- Immune Sera
- Interleukin-6
- Receptors, Interleukin
- Receptors, Interleukin-10
- Viral Proteins
- Interleukin-10