Summary
A histological and morphometric study demonstrated a relationship between vascular changes in the hepatic lobule and heroin consumption. To establish the role of hepatocytes in the genesis of sinusoidal dilatation, morphometric analysis was performed on ten drug abusers and eight controls. A total of 1800 hepatocytes, in 67 centrilobular areas, were analysed from biopsies from the total patient number. Computerized results of hepatocyte surface area, perimeter, maximum linear dimension and minimum linear dimension demonstrated no statistically significant difference for these variables, particularly for hepatocyte surface area (Controls: 268.66±95.25; drug abusers: 252.00±78.94,p=0.24), when the two groups of patients were compared. Hepatocyte morphology at the time of the biopsy was unaltered, although transaminase values were elevated for all drug abusers. It is, therefore, possible that the hepatocytes were not implicated in the pathogenesis of sinusoidal dilatation. This suggestion supports our previous results, which suggested that heroin was capable of inducing direct vascular hepatotoxicity.
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References
Baheri SA, Boyer JL (1974) Peliosis hepatis associated with androgenic-anabolic therapy. A severe form of hepatic injury. Ann Intern Med 81: 610–618
Borgia G, Crowell J, Cocchiararo M, Abrescia N, Lambiase G, Alfonso GD, Schreil W, Piazza M (1982) Ultrastructural changes in mouse liver cells: a morphometric study on the influence of morphine, heroin and cardiostenol. J Ultrastruct Res 80: 123–132
Chang YYH, Ho IK (1979) Effects of acute and continuous morphine administration on serum glutamate oxalacetate transaminase and glutamate pyruvate transaminase activities in the mouse. Biochem Pharmacol 28: 1373–1377
Czapar CA, Weldon-Linne CM, Moore DM, Rhone DP (1986) Peliosis hepatis in the acquired immunodeficiency syndrome. Arch Pathol Lab Med 110: 611–613
Dalich GM, Larson RE (1985) Temporal and dose-response features of monochlorobenzene hepatotoxicity in rats. Fundam Appl Toxicol 5: 105–116
Davion T, Capron JP, Andrejak M, Geoffroy P, Capron-Chivrac D, Quenum C (1984) Acute hepatitis due to carbamazepine (tegretol). Study of a case and review of the literature. Gastroenterol Clin Biol 8: 52–56
Dubuisson L, Bedin C, Boussarie L, Balabaud C, Bioulac-Sage P (1988) Liver sinusoids and hemodynamic disturbance. In: Bioulac-Sage P, Balabaud C (eds), Sinusoids in human liver: health and disease. Kupffer Cell Foundation, Netherlands, pp 279–297
Eklöw-L⇘stbom L, Moldéus P, Orrenius S (1986) On the mechanisms of glutathione depletion in hepatocytes exposed to morphine and ethylmorphine. Toxicology 42: 13–21
Elliot HW, Parker KD, Crim M, Wright JA, Nomof N (1971) Actions and metabolism of heroin administered by continuous intravenous infusion to man. Clin Pharmacol Ther 12: 806–814
Gorodetzky CW, Sapira JD, Jasinski DR, Martin WR (1968) Liver disease in narcotic addicts: the role of the drug. Clin Pharmacol Ther 9: 720–739
James RC, Goodman DR, Harbison RD (1982) Hepatic glutathione and hepatotoxicity: changes induced by selected narcotics. J Pharmacol Exp Ther 221: 708–714
Kunze KD, Porst H, Lohmann J, Tschopel L (1985) Liver lesions induced by dihydralazine and propranolol. Zentralbl Allg Pathol 130: 19–29
Lechón-Gomez MJ, Ponsoda X, Jover R, Fabra R, Trullenque R, Catell JV (1987) Hepatotoxicity of opioids morphine, heroin, meperidine, and methadone to cultured human hepatocytes. Mol Toxicol 1: 453–463
Litt IF, Cohen MI, Schonberg SK, Spigland I (1972) Liver disease in drug-using adolescent. J Pediatr 81: 238–242
Marks V, Chapple PAL (1967) Hepatic dysfunction in heroin and cocaine users. Br J Addict 62: 189–195
Nagamatsu K, Ohno Y, Ikebuchi H, Takahashi A, Terao T, Takanaka A (1986) Morphine metabolism in isolated rat hepatocytes and its implications for hepatotoxicity. Biochem Pharmacol 35: 3543–3548
Potter HP Jr, Cohen NN, Norris RF (1960) Chronic hepatic dysfunction in heroin addicts: possible role to carrier state of viral hepatitis. JAMA 174: 2049–2051
Roschlau G (1983) Dihydralazine-induced hepatitis with bridging necrosis. A clinical-pathologic survey of twenty cases. Zentralbl Allg Pathol 127: 385–393
Sawynok J (1986) The therapeutic use of heroin: a review of the pharmacological literature. Can J Physiol Pharmacol 64: 1–6
Schmidt G, Borsch G, Ricken D, Muller KM, Neuberger J (1985) Centrolobular liver cell necrosis following occupational halothane contact. Association with antibodies to halothane altered liver cell components. Z Gastroenterol 23: 192–197
Scoazec JY, Marche C, Girard PM, Houtmann J, Schneider AND, Saimot AG, Benhamou JP, Feldmann G (1988) Peliosis hepatis and sinusoidal dilatation during infection by the human immunodeficiency virus (HIV). An ultrastructural study. Am J Pathol 131: 38–47
Skoulis NP, James RC, Harbison RD, Roberts SM (1989) Depression of hepatic glutathione by opioid analgesic drugs in mice. Toxicol Appl Pharmacol 99: 139–147
Thuresen-Klein A, Wang-Yang J, HO IK (1978) Lipid accumulation in mouse hepatocytes after morphine exposure. Experientia 34: 773–774
Trigueiro de Araújo MS, Gérard F, Chossegros P, Porto LC, Barlet P, Grimaud J-A (1990) Vascular hepatotoxicity related to heroin addiction. Virchows Arch [A] 417: 497–503
Way EL, Kemp JW, Young JM, Grassetti DR (1960) The pharmacological effects of heroin in relationship to its rate of biotransformation. J Pharmacol Exp Ther 129: 144–154
Wisse E, De Zanger RB, Charels K, Van Der Smissen P, McCuskey RS (1985) The liver sieve: considerations concerning the structure and function of endothelial fenestrae, the sinusoidal wall and space of Disse. Hepatology 5: 683–692
Zafrani ES, Feldmann G (1988) Primary lesions of the hepatic sinusoid. In: Bioulac- Sage P, Balabaud C (eds), Sinusoids in human liver disease. Kupffer Cell Foundation, Netherlands, pp 207–222
Zafrani ES, Pinadeau Y, Dhumeaux D (1983) Drug-induced vascular lesions of the liver. Arch Intern Med 143: 495–502
Zak FG (1950) Peliosis hepatis. Am J Pathol 26: 1–14
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de Araújo, M.S.T., Gérard, F., Chossegros, P. et al. Lack of hepatocyte involvement in the genesis of the sinusoidal dilatation related to heroin addiction: a morphometric study. Vichows Archiv A Pathol Anat 420, 149–153 (1992). https://doi.org/10.1007/BF02358806
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DOI: https://doi.org/10.1007/BF02358806