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Enzyme analysis of genetic variation and relationships in diploid and polyploid taxa ofGalium (Rubiaceae)

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Abstract

Allozyme variation at 11 loci (with 37 alleles) was studied electrophoretically in seven outbreeding, closely related diploid and tetraploid taxa, seven from sect.Leptogalium and two from sect.Leiogalium. Whereas the sections are clearly distinct by several different alleles, aggregates, species and subspecies differ only in the frequency or presence/absence of common alleles. The resulting dendrogram suggests phylogenetic relationships and is supported by other multidisciplinary evidence. Tetraploids have originated independently in several groups, and there is evidence for tetrasomic inheritance and thus for autopolyploidy in spite of normal meiotic bivalent pairing and partly suspected hybrid origin. Tetraploids differ from related diploids only little in number of alleles and expected heterozygosity within populations, but clearly exhibit higher numbers of genotypes. This often corresponds to their greater morphological variability, increased adaptive flexibility, and better colonizing capacity compared to related diploids.

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References

  • Ayala, F. J., Powell, J. R., Tracey, M. L., Mourao, C. A., Perez-Salas, S., 1972: Enzyme variability in theDrosophila willistoni group. IV. Genetic variation in natural populations ofDrosophila willistoni. — Genetics70: 113–139.

    Google Scholar 

  • Brochmann, C., Soltis, D. E., Soltis, P. S., 1992: Electrophoretic relationships and phylogeny of nordic polyploids inDraba (Brassicaceae). — Pl. Syst. Evol.182: 35–70.

    Google Scholar 

  • Cavalli-Molina, S., Motta, E. P. V., Schiengold, M., Winge, H., 1989: Identical isoenzyme patterns in sib plants ofRelbunium hypocarpium (Rubiaceae). — Rev. Brasil. Genet.12: 361–368.

    Google Scholar 

  • Cai, Q., Macdonald, S. E., Chinnappa, C. C., 1990: Studies on theStellaria longipes complex (Caryophyllaceae): isozyme variability and the relationship betweenStellaria longipes andS. longifolia. — Pl. Syst. Evol.173: 129–141.

    Google Scholar 

  • Crawford, D. J., 1983: Phylogenetic and systematic inferences from electrophoretic studies. — InTanksley, S. D., Orton, T. J., (Eds): Isoenzymes in plant genetics and breeding, pp. 257–287. — Amsterdam: Elsevier.

    Google Scholar 

  • —, 1985: Electrophoretic data and plant speciation. — Syst. Bot.10: 405–416.

    Google Scholar 

  • —, 1990: Enzyme electrophoresis and plant systematics. — InSoltis, D. E., Soltis, P. S., (Eds): Isozymes in plant biology, pp. 146–164. — London: Chapman & Hall.

    Google Scholar 

  • —, 1984: Allozyme divergence and intraspecific variation inCoreopsis grandiflora (Compositae). — Syst. Bot.9: 219–225.

    Google Scholar 

  • Desrochers, A. M., Bohm, B. A., 1995: Biosystematic study ofLasthenia californica (Asteraceae). — Syst. Bot.20: 65–84.

    Google Scholar 

  • Ehrendorfer, F., 1949: Zur Phylogenie der GattungGalium I. Polyploidie und geographisch-ökologische Einheiten in der Gruppe desGalium pumilum Murray (Sect.Leptogalium Lange) im österreichischen Alpenraum. — Österr. Bot. Z.96: 109–138.

    Google Scholar 

  • —, 1962: Cytotaxonomische Beiträge zur Genese der mitteleuropäischen Flora und Vegetation. — Ber. Deutsch. Bot. Ges.75: 137–152.

    Google Scholar 

  • —, 1976:Rubiaceae, Galium. — InTutin, T. G., Heywood, V. H., Burges, N. A., Valentine, D. H., Walters, S. M., Webb, D. A., (Eds): Flora Europaea4: 14–36. — Cambridge: Cambridge University Press.

    Google Scholar 

  • Hamrick, J. L., 1990: Isozymes and the analysis of genetic structure in plant populations. — InSoltis, D. E., Soltis, P. S., (Eds): Isozymes in plant biology, pp. 87–105. — London: Chapman & Hall.

    Google Scholar 

  • —, 1989: Allozyme diversity in plant species. — InBrown, A. H. D., Clegg, M. T., Kahler, A. L., Weir, B. S., (Eds): Plant population genetics, breeding and genetic resources, pp. 43–63. — Sunderland, Ma.: Sinauer.

    Google Scholar 

  • —, 1979: Relationships between life history characteristics and electrophoretically detectable genetic variation in plants. — Annu. Rev. Ecol. Syst.10: 173–200.

    Google Scholar 

  • Harris, H., Hopkinson, D. A., 1976: Handbook of enzyme electrophoresis in human genetics. — Amsterdam: North-Holland.

    Google Scholar 

  • Hauber, D. P., 1986: Autotetraploidy inHaplopappus spinulosus hybrids: evidence from natural and synthetic tetraploids. — Amer. J. Bot.73: 1595–1606.

    Google Scholar 

  • Huber, W., Leuchtmann, A., 1992: Genetic differentiation of theErigeron species (Asteraceae) in the Alps: a case of unusual allozymic uniformity. — Pl. Syst. Evol.183: 1–16.

    Google Scholar 

  • Hurka, H., 1993: Isozymes in population genetic studies. — InLieth, H., Al Masoom, A., (Eds): Towards the rational use of high salinity tolerant plants,2, pp. 75–82. — The Netherlands: Kluwer.

    Google Scholar 

  • —, 1989: Aspartate aminotransferase isozyme in the genusCapsella (Brassicaceae): Subcellular location, gene duplication, and polymorphism. — Biochem. Gen.27: 77–90.

    Google Scholar 

  • Jaaska, V., 1994: Isoenzyme evidence on the systematics ofHordeum sectionMarina (Poaceae). — Pl. Syst. Evol.191: 213–226.

    Google Scholar 

  • Krendl, F., 1967: Cytotaxonomie derGalium mollugo-Gruppe in Mitteleuropas. — Österr. Bot. Z.114: 508–549.

    Google Scholar 

  • —, 1993: Chromosomenzahlen und geographische Verbreitung in der GattungGalium (Sect.Leptogalium-Rubiaceae). — Biosyst. Ecol. Ser.4: 51–112. — Wien: Österr. Akad. Wiss.

    Google Scholar 

  • Loveless, M. D., Hamrick, J. L., 1984: Ecological determinants of genetic structure in plant populations. — Annu. Rev. Ecol. Syst.15: 65–95.

    Google Scholar 

  • Lowrey, T. K., Crawford, D. J., 1985: Allozyme divergence and evolution inTetramolopium (Compositae, Asteraceae) in the Hawaiian Islands. — Syst. Bot.10: 64–72.

    Google Scholar 

  • Lumaret, R., 1985: Phenotypic and genotypic variation within and between populations of the polyploid complex,Dactylis glomerata L. — InHaeck, J., Woldendorp, J. W., (Eds): Structure and functioning of plant populations,2: phenotypic and genotypic variation in plant populations, pp. 343–353. — Amsterdam: North-Holland.

    Google Scholar 

  • —, 1987: Mise en évidence d'un écotype de dactyle (Dactylis glomerata L.) de pelouses dolomitiques subalpines dans les Grisons (Suisse): origine et échanges géniques avec les dactyles des prairies adjacentes. — Ecol. Plant8: 3–20.

    Google Scholar 

  • —, 1990: Phylogenetic relationships and gene flow between sympatric diploid and tetraploid plants ofDactylis glomerata (Gramineae). — Pl. Syst. Evol.169: 81–96.

    Google Scholar 

  • Macdonald, S. E., Chinnappa, C. C., 1988: Patterns of variation in theStellaria longipes complex: Effects of polyploidy and natural selection. — Amer. J. Bot.75: 1191–2000.

    Google Scholar 

  • Manen, J. F., Natali, A., Ehrendorfer, F., 1994: Phylogeny ofRubiaceae-Rubieae inferred from the sequence of a cpDNA intergene region. — Pl. Syst. Evol.190: 195–211.

    Google Scholar 

  • Mcleod, M. J., Guttman, S. I., Eshbaugh, W. H., Rayle, R. E., 1983: An electrophoretic study of evolution inCapsicum (Solanaceae). — Evolution37: 562–574.

    Google Scholar 

  • Meusel, H., Jäger, E. J., 1992: Vergleichende Chorologie der zentraleuropäischen Flora3. — Stuttgart: G. Fischer.

    Google Scholar 

  • Natali, A., Manen, J. F., Ehrendorfer, F., 1995: Phylogeny of theRubiaceae-Rubioideae, in particular the tribeRubieae: evidence from a non-coding chloroplast DNA sequence. — Ann. Missouri Bot. Gard.82: 428–439.

    Google Scholar 

  • Nei, M., 1972: Genetic distance between populations. — Amer. Nat.106: 283–292.

    Google Scholar 

  • Pedrola-Monfort, J., Caujapé-Castells, J., 1994: Allozymic and morphological relationships amongAndrocymbium gramineum, A. europaeum, andA. psammophilum (Colchicaceae). — Pl. Syst. Evol.191: 111–126.

    Google Scholar 

  • Qiu, Y. L., Parks, C. R., 1994: Disparity of allozyme variation level in threeMagnolia (Magnoliaceae) species from the southeastern United States. — Amer. J. Bot.81: 1300–1308.

    Google Scholar 

  • Raelson, J. V., Grant, W. F., 1988: Evaluation of hypotheses concerning the origin ofLotus corniculatus (Fabaceae) using isoenzyme data. — Theor. Appl. Genet.76: 267–276.

    Google Scholar 

  • Samuel, R., Pinsker, W., Ehrendorfer, F., 1990: Allozyme polymorphism in diploid and polyploid populations ofGalium. — Heredity65: 369–378.

    Google Scholar 

  • —, 1995: Electrophoretic analysis of genetic variation within and between populations ofQuercus cerris, Q. pubescens, Q. petraea andQ. robur (Fagaceae) from eastern Austria. — Bot. Acta108: 290–299.

    Google Scholar 

  • Shore, J. S., 1991: Tetrasomic inheritance and isozyme variation inTurnera ulmifolia vars.elegans Urb. andintermedia Urb. (Turneraceae). — Heredity66: 305–312.

    Google Scholar 

  • Šipošová, H., 1987: A taxonomic-chorological study of theGalium pumilum Murray s.l. in Slovakia. — Acta Bot. Slov., ser. A. Tax. Geobot.10: 97–169.

    Google Scholar 

  • Sneath, P. H. A., Sokal, R. R., 1973: Numerical taxonomy. — San Francisco: Freeman.

    Google Scholar 

  • Soltis, D. E., Soltis, P. S., 1988: Electrophoretic evidence for tetrasomic segregation inTolmiea menziesii (Saxifragaceae). — Heredity60: 375–382.

    Google Scholar 

  • —, 1990: Isoenzymes in plant biology. — London: Chapman & Hall.

    Google Scholar 

  • —, 1993: Molecular data and the dynamic nature of polyploidy. — Crit. Rev. Pl. Sc.12: 243–273.

    Google Scholar 

  • Wells, T. C., Bohm, B. A., 1994: Isozyme variation in North AmericanMenziesia (Ericaceae). — Syst. Bot.19: 407–423.

    Google Scholar 

  • Wilson, H. D., Barber, S. C., Walter, T., 1983: Loss of duplicate gene expression in tetraploidChenopodium. — Biochem. Syst. Ecol.11: 7–13.

    Google Scholar 

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Authors and Affiliations

  1. Institut für Botanik und Botanischer Garten, Universität Wien, Rennweg 14, A-1030, Wien, Austria

    Friedrich Ehrendorfer & Rosabelle Samuel

  2. Institut für Allgemeine Biologie, AG Genetik, Universität Wien, Währinger Straße 17, A-1090, Wien, Austria

    Wilhelm Pinsker

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  1. Friedrich Ehrendorfer
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  2. Rosabelle Samuel
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  3. Wilhelm Pinsker
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Ehrendorfer, F., Samuel, R. & Pinsker, W. Enzyme analysis of genetic variation and relationships in diploid and polyploid taxa ofGalium (Rubiaceae). Pl Syst Evol 202, 121–135 (1996). https://doi.org/10.1007/BF00985821

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  • DOI: https://doi.org/10.1007/BF00985821

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