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Genetic variation in natural populations of Capparis from Turkey, as revealed by RAPD analysis

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Abstract

In this study, the genetic diversity of 15 Turkish natural Capparis populations was screened using the randomly amplified polymorphic DNA analysis (RAPD) technique. Ten RAPD primers produced 98 loci, 73 of which were polymorphic. The binary RAPD data were computed using the POPGENE (version 1.31), a genetic data analysis software program. According to genetic diversity analysis at locus level, the total genetic diversity (H T) and genetic diversity within population (H s) were detected as 0.16 and 0.12, respectively. The genetic differentiation (G ST) and gene flow (N m) between populations were observed as 0.22 and 1.79, respectively. The mean number of allele per locus (n a), the mean number of effective allele (n ea), and the mean value of genetic diversity (H e) were determined as 2, 1.20, and 0.16, respectively. According to Pearson’s correlation analysis, the mean number of allele had a strong negative correlation with wind and a strong positive correlation with rain. According to multiple regression analysis, eco-geographical factors had a significant effect on the mean number of allele, the mean number of effective allele, and the mean value of genetic diversity. The principal component analysis revealed 87.42 % of total genetic variation. The principal coordinate analysis displayed the separation of population according to genetic distances based on dissimilarities matrix values on a scattered plot graph. Five different varieties, Capparis spinosa L. var. spinosa, var aegyptia and var. canescens, and Capparis ovata Desf. var. palaestina, and var. herbacea were identified in this study. Intermediate forms of plants were observed among the specimens.

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References

  • Abdel-Mawgood AL, Assaeed AM, Al-Abdallatif TI (2006) Application of RAPD technique for the conservation of an isolated population of Capparis decidua Alexandria. J Agric Res 51:171–177

    Google Scholar 

  • Abdel-Mawgood AL, Assaeed AM, Jakse J (2010) Genetic Structure and Diversity Within and Among Six Populations of Capparis decidua (Forssk.) Edgew. in Saudi Arabia. Plant Syst Evol 9(36):79–93

    Google Scholar 

  • Al-Gohary IH (1982) Morphological studies on the Capparidaceae in Egypt. M.Sc. Thesis, Faculty of Science, Ain Shams University, Cairo

  • Andrieu E, Dornier A, Rouifed S, Schatz B, Cheptou PO (2009) The town Crepis and the country Crepis: how does fragmentation affect a plant-pollinator interaction? Acta Oecologica 35:1–7. doi:10.1016/j.actao.2008.07.002

    Article  Google Scholar 

  • Barbera G, Di Lorenzo RD (1984) The caper culture in Italy. Acta Horticulturae 144:167–171

    Google Scholar 

  • Bawa KS, Bulloch SH, Perry DR, Coville RE, Grayum MH (1985) Reproduction biology of tropical lowland rain forest tree. II. Pollination system. Am J Bot 72:346–356

    Article  Google Scholar 

  • Coode MJE (1965) Capparis L. In: Davis PH (ed.) Flora of Turkey and the East Aegean Islands, vol 1. Edinburgh University Press, Edinburgh, pp 496–498

  • Davis PH (1965–1985) Flora of Turkey and the East Aegean Island, vol 1–9. Edinburg University Press, Edinburg

  • El-Karemy ZAR (2001) Capparaceae in the flora of Egypt. Taeckholmia 21:257–267

    Google Scholar 

  • Fausto JA Jr, Eckhart VM, Geber MA (2001) Reproductive assurance and the evolutionary ecology of self-pollination in Clarkia xantiana(Onagraceae). Am J Bot 88:1794–1800

    Article  PubMed  Google Scholar 

  • Fici S (1993) Taxonomic and Chorological notes on the genera Boscia Lam. Cadaba Foressk and Capparis L. (Capparaceae) in Somalia. Webbia 47(1):149–162

    Article  Google Scholar 

  • Fici S (2011) Capparis tchaourembensis, a new species of Capparaceae from Mayotte, Comororian Archipelago. Kew Bull 66:299–301

    Article  Google Scholar 

  • Fici S, Gianguzzi L (1997) Diversity and conservation of wild and cultivated Capparis in Sicily. Bocconea, 7:437–443. ISSN 1120-4060

    Google Scholar 

  • Harper JL (1970) The shapes and sizes of seeds. Annu Rev Ecol Syst 1:327–356

    Article  Google Scholar 

  • Heywood VH (1964) Capparis L. In: Tutin TG, Heywood VH, Burges NA, Valentine D H, Walters SM, Webb DA (eds) Flora Europaea vol 1. Cambridge University Press, Cambridge, p 259

  • Heywood VH (1993) Flowering plants of the world. Oxford University Press, New York

    Google Scholar 

  • Higton RN, Akeroyd JR (1991) Variation in Capparis spinosa L. in Europe. Flora Europaea 106(2):104–112

    Google Scholar 

  • Inocencio C, Cowan RS, Alcaraz F, Rivera D, Fay ML (2005) AFLP fingerprinting in Capparis subgenus Capparis related to the commercial sources of capers. Genet Resour Crop Evol 52(2):137–144

    Article  CAS  Google Scholar 

  • Inocencio C, Rivera D, Obón MC, Alcaraz F, Barreña A (2006) A systematic revision of Capparis section Capparis (Capparaceae). Ann Mo Bot Gard 93(1):122–149

    Article  Google Scholar 

  • Jacobs M (1965) The genus Capparis (Capparaceae) from the Indus to the Pacific. Blumea 12(3):385–541

    Google Scholar 

  • Jennersten O (1988) Pollination in Dianthus deltoids (Caryophyllaceae): effects of habitat fragmentation on visitation and seed set. Conserv Biol 2:359–366

    Article  Google Scholar 

  • Keller LF, Waller DM (2002) Inbreeding effects in wild populations. Trends Ecol Evol 17:230–241

    Article  Google Scholar 

  • Kers LE (2003) Capparaceae. In: Kubitzki K (Series Editor): The families and genera of vascular plants, vol 5: Kubitzki K, Bayer C (eds) Springer, Berlin, pp 36–56. ISBN 3-540-42873-9

  • Lienert J (2004) Habitat fragmentation effects on fitness of plant populations—a review. J Nat Conserv 12:53–72

    Article  Google Scholar 

  • Mabberley DJ (1987) The Plant-Book. A Portable Dictionary of the Higher Plants. Cambridge University Press, Cambridge

    Google Scholar 

  • Moubasher H, Abd El-Ghani MM, Kamel W, Mansi M, El-Bous M (2011) Taxonomic considerations among and within some Egyptian taxa of Capparis and related genera (Capparaceae) as revealed by RAPD fingerprinting. Collectanea Botanica 30:29–35. doi:10.3989/collectbot.2011.v30.003

    Article  Google Scholar 

  • Nei M (1972) Nei’s original measures of genetic identity and genetic distance. Am Nat 106:283–292

    Article  Google Scholar 

  • Nei M (1973) Analysis of gene diversity in subdivided populations (population structure/genetic variability/heterozygosity/gene differentiation). Proc Natl Acad Sci USA 70(12):3321–3323

    Article  PubMed  CAS  Google Scholar 

  • Nosrati H, Feizi MAH, Mazinani M, Haghighi AR (2012) Effect of population size on genetic variation levels in Capparis spinosa (Capparaceae) detected by RAPDs. Eur Asian J BioSci 6:70–75

    Article  Google Scholar 

  • Reed DH, Frankham R (2003) Correlation between fitness and genetic diversity. Conserv Biol 17:230–237

    Article  Google Scholar 

  • Saifi N, Ibijbijen J, Echchgadda D (2011) Genetic diversity of caper plant (Capparis ssp.) from North Morocco. J Food Agric Environ 9(3, 4):299–304

    Google Scholar 

  • Skogsmyr I, Lankinen A (2002) Sexual selection: an evolutionary force in plants? Biol Rev Camb Philos Soc 77:537–562. doi:10.1017/S1464793102005973

    Article  PubMed  Google Scholar 

  • Tilli N, Saadaoui E, Sakouhi F, Elfalleh W, El-Gazzah M, Triki S, Khaldi A (2011) Morphology and chemical composition of Tunisian caper seeds: variability and population profiling. Afr J Biotechnol 10(10):2112–2118

    Google Scholar 

  • Vyas GK, Sharma R, Kumar V, Sharma TB, Khandelwal V (2009) Diversity analysis of Capparis decidua (Forssk.) Edgew. using biochemical and molecular parameters. Genet Resour Crop Evol 56:905–911

    Article  CAS  Google Scholar 

  • Wilcock C, Neiland MRM (2002) Pollination failure in plants: why it happens and when it matters. Trends Plant Sci 7:270–277

    Article  PubMed  CAS  Google Scholar 

  • Yeh FC, Yang RC, Boyle T, Ye ZH, Mao JX (1997) POPGENE (version 1.32): The 523 user-friendly shareware for population genetic analysis. Molecular Biology and 524 Biotechnology Centre, University of Alberta Canada

  • Zhang T, Tan DY (2009) An examination of the function of male flowers in an andromonoecious shrub Capparis spinosa. J Integr Plant Biol 51:316–324

    Article  PubMed  Google Scholar 

  • Zohary M (1960) The species of Capparis in the Mediterranean and the near eastern countries. Bull Res Counc Israel 80:49–65

    Google Scholar 

Download references

Acknowledgments

This is the master thesis project of Aslı Kara and supported by a grant, which was provided by the Scientific Research Projects Department (BAP) of Hitit University with project number of FEF01.10.004. We are grateful for the collaboration of the General Directorate of Agricultural Research and Policy unit of the Republic of Turkey, Ministry of Food, Agriculture, and Livestock for providing technical assistance for field studies and special thanks to Dr. Fetullah Tekin for his contribution in the collection of Capparis seeds with us. We are also thankful to Prof. Dr. Şinasi Yıldırımlı from Hacettepe University for contribution to identification of plants according to Hacettepe University Herbarium (HUB) material. We are also giving special thanks to Dr. Silvio Fici from University of Palermo in Italy for his great contribution to improvement of this manuscript.

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  1. Department of Biology, Faculty of Art and Sciences, Hitit University, Ulukavak Mah. Çiftlik Çayırı Cd. No: 61, Çorum, Turkey

    Özlem Özbek & Aslı Kara

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  1. Özlem Özbek
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  2. Aslı Kara
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Correspondence to Özlem Özbek.

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Özbek, Ö., Kara, A. Genetic variation in natural populations of Capparis from Turkey, as revealed by RAPD analysis. Plant Syst Evol 299, 1911–1933 (2013). https://doi.org/10.1007/s00606-013-0848-0

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