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Molecular analysis of genetic diversity in melon landraces (Cucumis melo L.) from Myanmar and their relationship with melon germplasm from East and South Asia

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Abstract

Genetic diversity of Myanmar melon was evaluated by analysis of 27 RAPD markers and morphological characters using 41 accessions of melon landraces of which 36 accessions were small-seed type. The gene diversity was 0.239, higher than for group Conomon from East Asia and equivalent to Indian melon populations. Melon accessions were classified into six major clusters. The largest cluster IV comprised mainly group Conomon which was closely related to cluster V consisting of mainly group Agrestis. Most of the accessions of group Cantalupensis were grouped into clusters II or VII and were distantly related to groups Conomon and Agrestis. The genetic relationship to melon accessions from neighboring countries was analyzed. The 24 accessions of clusters IV and V were mostly clustered together with small-seed type melon of India, but the 14 accessions of clusters VI and VII were mostly clustered together with large-seed type melon of India. These results indicated that the genetic diversity of Indian melon is conserved in Myanmar. Genetic introgression among melon groups through spontaneous hybridization was also indicated and was considered important to maintain or increase the genetic diversity in Myanmar.

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References

  • Akashi Y, Fukuda N, Wako T, Masuda M, Kato K (2002) Genetic variation and phylogenetic relationships in East and South Asian melons Cucumis melo L., based on the analysis of five isozymes. Euphytica 125:385–396. doi:10.1023/A:1016086206423

    Article  CAS  Google Scholar 

  • Apostol BL, Black WCIV, Miller BR, Reiter P, Beaty BJ (1993) Estimation of the number of full sibling families at an oviposition site using RAPD-PCR markers: applications to the mosquito Aedes aegypti. Theor Appl Genet 86:991–1000. doi:10.1007/BF00211052

    Article  CAS  Google Scholar 

  • Bates DM, Robinson RW (1995) Cucumber, melons and water-melons, Cucumis and Citrullus (Cucurbitaceae). In: Smartt J, Simmonds NW (eds) Evolution of crop plants, vol 10158. Wiley, New York, pp 89–111

    Google Scholar 

  • Dhillon NPS, Ranjana R, Singh K, Eduardo I, Monforte AJ, Pitrat M, Dhillon NK, Singh PP (2007) Diversity among landraces of Indian snapmelon (Cucumis melo var. momordica). Genet Resour Crop Evol 54:1267–1283. doi:10.1007/s10722-006-9108-2

    Article  Google Scholar 

  • Fujishita N (1983) Genetic diversity and phylogenetic differentiation in melon. Curr Top Plant Breed 24:3–21 in Japanese

    Google Scholar 

  • Garcia-Mas J, Oliver M, Gomez-Paniagua H, de Vicente MC (2000) Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon. Theor Appl Genet 101:860–864. doi:10.1007/s001220051553

    Article  CAS  Google Scholar 

  • Gower JC (1966) Some distance properties of latent root and vector methods used in multivariate analysis. Biometrika 53:325–338

    Google Scholar 

  • Jeffrey C (2001) Cucurbitaceae. In: Hanelt P, Institute of Plant Genetics and Crop Plant Research (eds) Mansfeld’s encyclopedia on agricultural and horticultural crops. Springer, Berlin, pp 1510–1557

    Google Scholar 

  • Kato K, Yoshino H, Matsuura S, Akashi Y, Tanaka K (2006) Cucurbitaceae crop. In: Takeda K (ed) Genetic assay and study of crop germplasm in and around China, 3rd edn. Okayama University, Okayama, pp 69–85

    Google Scholar 

  • Kitamura S (1950) Notes on Cucumis of far East. Acta Phytotax Geobot 14:41–44

    Google Scholar 

  • Lopez-Sese AI, Staub JE, Gomez-Gullamon ML (2003) Genetic anysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular marker array and reference accessions. Theor Appl Genet 108:41–52. doi:10.1007/s00122-003-1404-z

    Article  CAS  PubMed  Google Scholar 

  • McCreight JD, Staub JE, Lopez-Sese A, Chung S (2004) Isozyme variation in Indian and Chinese melon (Cucumis melo L.) germplasm collections. J Am Soc Hortic Sci 129:811–818

    CAS  Google Scholar 

  • Ministry of Agricultural Services (MAS) (2006) Annual report of horticulture. MAS, Myanmar

    Google Scholar 

  • Mliki A, Staub JE, Sun Z, Ghorbel A (2001) Genetic diversity in melon (Cucumis melo L.): an evaluation of African germplasm. Genet Resour Crop Evol 48:587–597. doi:10.1023/A:1013840517032

    Article  Google Scholar 

  • Monforte AJ, Garcia-Mas J, Arus P (2003) Genetic variability in melon based on microsatellite variation. Plant Breed 122:153–157. doi:10.1046/j.1439-0523.2003.00848.x

    Article  Google Scholar 

  • Munger HM, Robinson RW (1991) Nomenclature of Cucumis melo L. Cucurbit Genet Coop Rep 14:43–44

    Google Scholar 

  • Murray GC, Thompson WF (1980) Rapid isolation of high molecular weight DNA. Nucleic Acids Res 8:4321–4325. doi:10.1093/nar/8.19.4321

    Article  CAS  PubMed  Google Scholar 

  • Nakata E, Staub JE, Lopez-Sese AI, Katzir N (2005) Genetic diversity of Japanese melon cultivars (Cucumis melo L.) as assessed by random amplified polymorphic DNA and simple sequence repeat markers. Genet Resour Crop Evol 52:405–419. doi:10.1007/s10722-005-2258-9

    Article  CAS  Google Scholar 

  • Naudin C (1859) Especes et des varietes du genra cucumis. Sci Nat 11:5–87

    Google Scholar 

  • Nei M (1972) Genetic distance between populations. Am Nat 106:283–292. doi:10.1086/282771

    Article  Google Scholar 

  • Neuhausen SL (1992) Evaluation of restriction fragment length polymorphism in Cucumis melo. Theor Appl Genet 83:379–384. doi:10.1007/BF00224286

    Article  Google Scholar 

  • Nybom H (2004) Comparison of different nuclear DNA makers for estimating intraspecific genetic diversity in plants. Mol Ecol 13:1143–1155. doi:10.1111/j.1365-294X.2004.02141.x

    Article  CAS  PubMed  Google Scholar 

  • Pitrat M, Hanelt P, Hammer K (2000) Some comments on intraspecific classification of cultivars of melon. Proc Cucurbitaceae 2000:29–36

    Google Scholar 

  • Staub JE, Lopez-Sese AI, Fanourakis N (2004) Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins. Euphytica 136:151–166. doi:10.1023/B:EUPH.0000030667.63614.bd

    Article  CAS  Google Scholar 

  • Tanaka K, Nishitani A, Akashi Y, Nishida H, Yoshino H, Kato K (2007) Molecular characterization of South and East Asian melon, Cucumis melo L., and the origin of group Conomon var. makuwa and var. conomon revealed by RAPD analysis. Euphytica 153:233–247. doi:10.1007/s10681-006-9259-4

    Article  CAS  Google Scholar 

  • Weir BS (1996) Genetic data analysis II. Sinauer Associate Inc. Publisher, Massachusetts

    Google Scholar 

  • Yashiro K, Iwata H, Akashi Y, Tomita K, Kuzuya M, Tsumura Y, Kato K (2005) Genetic relationship among East and South Asian melon (Cucumis melo L.) revealed by AFLP analysis. Breed Sci 55:197–206. doi:10.1270/jsbbs.55.197

    Article  CAS  Google Scholar 

  • Zhivotovsky LA (1999) Estimating population structure in diploids with multilocus dominant DNA makers. Mol Ecol 8:907–913. doi:10.1046/j.1365-294x.1999.00620.x

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We would like to thank Dr. Sakata, National Institute of Vegetable and Tea Science (NIVTS), Japan, for kindly supplying the seeds. This study was partly supported by a Grant-in-Aid for International Scientific Research of Ministry of Education, Science, Culture and Sports, Japan (No. 19255009), entitled “Genetic Assay and Study of Crop Germplasm In and Around China”. This is Contribution number 14 from the Sato Project of Research Institute for Humanity and Nature (RIHN), Japan.

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Correspondence to Kenji Kato.

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Yi, S.S., Akashi, Y., Tanaka, K. et al. Molecular analysis of genetic diversity in melon landraces (Cucumis melo L.) from Myanmar and their relationship with melon germplasm from East and South Asia. Genet Resour Crop Evol 56, 1149–1161 (2009). https://doi.org/10.1007/s10722-009-9438-y

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