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Las Alpujarras region (South East Spain) HLA genes study: evidence of a probable success of 17th century repopulation from North Spain

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Abstract

Conquest of Granada Muslim Kingdom (1492 AD) finished with Muslim occupation; they were mostly North African Berbers who had reached Iberia by 711 AD. A politics of Iberian Christianization followed after this date: Jewish were expelled in 1492 and Moriscos (Spaniards practicing Muslim religion or speaking Arab) were expelled from all Spanish territory on 1609 AD. Las Alpujarras is a southern Spain mountainous secluded region, which underwent a repopulation from North Spain and a specific Muslim (Moriscos)–Christian war took place according to historical records. Both Las Alpujarras repopulation by northern Iberians and Moriscos expulsion success have been debated and are regarded as non-clarified episodes. In this study, we have addressed the question whether the repopulation succeeded by determining HLA genes of present day Las Alpujarras inhabitants and compared with those of other Mediterranean populations HLA frequencies and genealogies. HLA frequencies show ambiguous results because of extant HLA similar gene frequencies there exist in North Africa and Spain. This is reflected by the finding of North and South western Mediterraneans close relatedness of HLA dendrograms and correspondence analyses. However, the genealogical study of extended HLA haplotypes particularly Alpujarran high frequency of HLA-A29-B44-DRB1*0701-DQA1*02-DQB1*02 (not found in Algerians but frequent in North and Central Spain) and Alpujarran low frequency extended haplotype HLA-A3-B7-DRB1*1501-DQA1*0102-DQB1*0602 (frequent in North Europe) reveals that a significant HLA gene flow from North Spain is observed in present day Alpujarrans: both haplotypes are characteristic of North Spain and North Europe, respectively. This may indicate that enforced Alpujarran repopulation from North Spain may have been a success, which was started by Spanish King Philip II in 1571 AD.

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References

  1. Gomez-Casado E, del Moral P, Martinez-Laso J, Garcia-Gomez A, Allende L, Silvera-Redondo C, Longas J, Gonzalez-Hevilla M, Kandil M, Zamora J, Arnaiz-Villena A (2000) HLA genes in Arabic-speaking Moroccans: close relatedness to Berbers and Iberians. Tissue Antigens 55:239–249

    Article  PubMed  CAS  Google Scholar 

  2. Vazquez Hoys AM (2008) Las golondrinas de Tartessos. Editorial Almazara, Madrid

    Google Scholar 

  3. Pellon JR (2006) Iberos-Dictionary. Editorial Espasa Calpe, Madrid

    Google Scholar 

  4. Maluquer de Motes J (1970) Tartessos. Editorial Destino, Barcelona

    Google Scholar 

  5. Lopez-Davalillo Y (1999) Atlas histórico de España y Portugal. Editorial Sintesis, Madrid

    Google Scholar 

  6. Hurtado de Mendoza D (1627) Guerra de Granada. Electronic Book Biblioteca Virtual Miguel de Cervantes, Alicante

    Google Scholar 

  7. Vincent B (2006) El Río Morisco. University of Valencia Press, Valencia

    Google Scholar 

  8. Arnaiz-Villena A, Timon M, Corell A, Perez-Aciego P, Martin-Villa JM, Regueiro JR (1992) Primary immunodeficiency caused by mutations in the gene encoding the CD3-gamma subunit of the T-lymphocyte receptor. New Engl J Med 327:529–533

    Article  PubMed  CAS  Google Scholar 

  9. Excoffier L, Slatkin M (1995) Maximum-likelihood estimation of molecular haplotype frequencies in a diploid population. Mol Biol Evol 12:921–927

    PubMed  CAS  Google Scholar 

  10. Mattiuz PL, Ihde D, Piazza A, Ceppelini R, Wodmer WF (1972) New approaches to the population genetics and segregation analysis of the HLA system. Munksgaard, Copenhangen

    Google Scholar 

  11. Imanishi T, Akaza T, Kimura A, Tokunaga K, Gojobori T (1992) Estimation of allele and haplotype frequencies for HLA and complement loci. In: Tsuji K, Aizawa M, Sasazuki T (eds) HLA 1991, 1st edn. Oxford University Press, Oxford, pp 76–79

    Google Scholar 

  12. Clayton J, Lonjou C (1997) Allele and haplotype frequencies for HLA loci in various ethnic groups. In: Charron D (ed) Genetic diversity of HLA. Functional and medical implications, 1st edn. EDK, Paris, pp 665–820

    Google Scholar 

  13. Arnaiz-Villena A, Martinez-Laso J, Gomez-Casado E, Diaz-Campos N, Santos P, Martinho A, Breda-Coimbra H (1997) Relatedness among Basques, Portuguese, Spaniards, and Algerians studied by HLA allelic frequencies and haplotypes. Immunogenetics 47:37–43

    Article  PubMed  CAS  Google Scholar 

  14. Imanishi T, Akaza T, Kimura A, Tokunaga K, Gojobori T (1992) Allele and haplotype frequencies for HLA and complement loci in various ethnic groups. In: Tsuji K, Aizawa M, Sasazuki T (eds) HLA 1991, 1st edn. Oxford University Press, Oxford, pp 1065–1220

    Google Scholar 

  15. Middleton D, Menchaca L, Rood H, Komerofsky R (2003) New allele frequency database. Tissue Antigens 61:403–407. http://www.allelefrequencies.net

    Google Scholar 

  16. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  17. Nei M (1972) Genetic distances between populations. Am Nat 106:283

    Article  Google Scholar 

  18. Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323

    Article  PubMed  CAS  Google Scholar 

  19. Nei M, Tajima F, Tateno Y (1983) Accuracy of estimated phylogenetic trees from molecular data, II. Gene frequency data. J Mol Evol 19:153–170

    Article  PubMed  CAS  Google Scholar 

  20. Young FW, Bann CM (1996) A visual statistics system. In: Stine RA, Fox J (eds) Statistical computing environments for social researches. Sage Publications, London, pp 207–236

    Google Scholar 

  21. Bodmer JG, Marsh SGE, Albert ED, Bodmer WF, Dupont B, Erlich HA, March B, Mayr WR, Parham P, Sasazuki T, Schreuder GMTH, Strominger JL, Svejgaard A, Terasaki PI (1992) Nomenclature for factors of the HLA system 1991. In: Tsuji K, Aizawa M, Sasazuki T (eds) HLA 1991, 1st edn. Oxford University Press, Oxford, pp 17–31

    Google Scholar 

  22. The Anthony Nolan Trust (2009) The Anthony Nolan Trust. http://www.anthonynolan.org.uk. Accessed 1 August 2010

  23. Gomez-Prieto P, Parga-Lozano C, Rey D, Moreno E, Arnaiz-Villena A (2010) HLA-G, -F and -E: polymorphism, function, and evolution. In: Mehra NK (ed) The HLA complex in biology and medicine. A resource book, 1st edn. Jaypee Brothers Medical Publishers, New Delhi, pp 159–174

    Google Scholar 

  24. Moscoso J, Crawford MH, Vicario JL, Zlojutro M, Serrano-Vela JI, Reguera R, Arnaiz-Villena A (2008) HLA genes of Aleutian islanders living between Alaska (USA) and Kamchatka (Russia) suggest a possible southern Siberia origin. Mol Immunol 45:1018–1026

    Article  PubMed  CAS  Google Scholar 

  25. Gomez-Casado E, Martinez-Laso J, Moscoso J, Zamora J, Martin-Villa M, Perez-Blas M, Lopez-Santalla M, Lucas GP, Silvera C, Lowy E, Arnaiz-Villena A (2003) Origin of Mayans according to HLA genes and the uniqueness of Amerindians. Tissue Antigens 61:425–436

    Article  PubMed  CAS  Google Scholar 

  26. Arnaiz-Villena A, Parga-Lozano C, Moreno E, Areces C, Rey D, Gomez-Prieto P (2010) The origin of Amerindians and the peopling of the Americas according to HLA genes: admixture with Asian and Pacific people. Curr Genomics 11:103–114

    Article  PubMed  CAS  Google Scholar 

  27. Martinez-Laso J, de Juan D, Martinez-Quiles N, Gomez-Casado E, Cuadrado E, Arnaiz-Villena A (1995) The contribution of the HLA-A, -B, -C and -DR, -DQ DNA typing to the study of the origins of Spaniards and Basques. Tissue Antigens 45:237–245

    Article  PubMed  CAS  Google Scholar 

  28. Arnaiz-Villena A, Gomez-Casado E, Martinez-Laso J (2002) Population genetic relationships between Mediterranean populations determined by HLA allele distribution and a historic perspective. Tissue Antigens 60:111–121

    Article  PubMed  CAS  Google Scholar 

  29. Martinez-Laso J, Gazit E, Gomez-Casado E, Morales P, Martinez-Quiles N, Alvarez M, Martin-Villa JM, Fernandez V, Arnaiz-Villena A (1996) HLA DR and DQ polymorphism in Ashkenazi and non-Ashkenazi Jews: comparison with other Mediterraneans. Tissue Antigens 47:63–71

    Article  PubMed  CAS  Google Scholar 

  30. Izaabel H, Garchon HJ, Caillat-Zucman S, Beaurain G, Akhayat O, Bach JF, Sanchez-Mazas A (1998) HLA class II DNA polymorphism in a Moroccan population from the Souss, Agadir area. Tissue Antigens 51:106–110

    Article  PubMed  CAS  Google Scholar 

  31. Arnaiz-Villena A, Dimitroski K, Pacho A, Moscoso J, Gomez-Casado E, Silvera-Redondo C, Varela P, Blagoevska M, Zdravkovska V, Martinez-Laso J (2001) HLA genes in Macedonians and the sub-Saharan origin of the Greeks. Tissue Antigens 57:118–127

    Article  PubMed  CAS  Google Scholar 

  32. Arnaiz-Villena A, Benmamar D, Alvarez M, Diaz-Campos N, Varela P, Gomez-Casado E, Martinez-Laso J (1995) HLA allele and haplotype frequencies in Algerians. Relatedness to Spaniards and Basques. Hum Immunol 43:259–268

    Article  PubMed  CAS  Google Scholar 

  33. Gaibar M, Esteban E, Moral P, Gomez-Gallego F, Santiago C, Bandres F, Luna F, Fernandez-Santander A (2010) STR genetic diversity in a Mediterranean population from the south of the Iberian peninsula. Ann Hum Biol 37:253–266

    Article  PubMed  Google Scholar 

  34. Fernandez-Santander A, Luna F, Santiago C, Rodriguez MT, Bandres F, Ruiz JR, Lucia A, Gomez-Gallego F (2010) CYP2D6 polymorphism screening in a selected population of Spain (La Alpujarra): no effect of geographical isolation. Ann Hum Biol 37:267–273

    Article  PubMed  Google Scholar 

  35. Sanchez-Velasco P, Gomez-Casado E, Martinez-Laso J, Moscoso J, Zamora J, Lowy E, Silvera C, Cemborain A, Leyva-Cobian F, Arnaiz-Villena A (2003) HLA alleles in isolated populations from north Spain: origin of the Basques and the ancient Iberians. Tissue Antigens 61:384–392

    Article  PubMed  CAS  Google Scholar 

  36. Degos L, Dausset J (1974) Human migrations and linkage disequilibrium of HLA system. Immunogenetics 1:195–210

    Article  Google Scholar 

  37. Arnaiz-Villena A, Alonso-Garcia J (2007) Diccionario Ibérico-Euskera-Castellano. Ediciones Fundación Estudios Genéticos y Lingüísticos, Madrid

    Google Scholar 

  38. Arnaiz-Villena A, Alonso-Garcia J (2008) Diccionario Etrusco-Euskera-Castellano. Ediciones Fundación Estudios Genéticos y Lingüísticos, Madrid

    Google Scholar 

  39. Arnaiz-Villena A, Alonso-Garcia J (2000) The Usko-Mediterranean languages. Kluwer Academic-Plenum Publishers, New York

    Google Scholar 

  40. Ruhlen M (1994) The origin of language. Wiley, New York

    Google Scholar 

  41. Fernandez-Santander A, Kandil M, Luna F, Esteban E, Gimenez F, Zaoui D, Moral P (1999) Genetic relationships between southeastern Spain and Morocco: new data on ABO, RH, MNSs, and DUFFY polymorphisms. Am J Hum Biol 11:745–752

    Article  PubMed  Google Scholar 

  42. Roitberg-Tambur A, Witt CS, Friedmann A, Safirman C, Sherman L, Battat S, Nelken D, Brautbar C (1995) Comparative analysis of HLA polymorphism at the serologic and molecular level in Moroccan and Ashkenazi Jews. Tissue Antigens 46:104–110

    Article  PubMed  CAS  Google Scholar 

  43. Muro M, Marin L, Torio A, Moya-Quiles MR, Minguela A, Rosique-Roman J, Sanchis MJ, Garcia-Calatayud MC, Garcia-Alonso AM, Alvarez-Lopez MR (2001) HLA polymorphism in the Murcia population (Spain): in the cradle of the archaeologic Iberians. Hum Immunol 62:910–921

    Article  PubMed  CAS  Google Scholar 

  44. Arnaiz-Villena A, Iliakis P, Gonzalez-Hevilla M, Longas J, Gomez-Casado E, Sfyridaki K, Trapaga J, Silvera-Redondo C, Matsouka C, Martinez-Laso J (1999) The origin of Cretan populations as determined by characterization of HLA alleles. Tissue Antigens 53:213–226

    Article  PubMed  CAS  Google Scholar 

  45. Arnaiz-Villena A, Elaiwa N, Silvera C, Rostom A, Moscoso J, Gomez-Casado E, Allende L, Varela P, Martinez-Laso J (2001) The origin of Palestinians and their genetic relatedness with other Mediterranean populations. http://kinoko.c.u-tokyo.ac.jp/~duraid/stolen_science/. Accessed 3 August 2010

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Acknowledgments

This study was supported in part by grants from the Spanish Ministry of Health (FISS PI051039 and PI080838), Spanish Ministry of Foreign Affairs (A/9134/07 and A/17727/08), and three different Mutua Madrileña Automovilística grants.

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Authors and Affiliations

  1. Department of Immunology, School of Medicine, Universidad Complutense, The Madrid Regional Blood Center, Av. Democracia s/n, 28032, Madrid, Spain

    Javier Longás, Diego Rey, Cristina Areces, Eduardo Gómez Casado, Carlos Parga-Lozano, Mercedes Enriquez de Salamanca & Antonio Arnaiz-Villena

  2. Unidad de Inmunoterapia Celular, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain

    Jorge Martínez-Laso

  3. Departamento de Zoología y Antropología Física, Universidad Complutense de Madrid, Madrid, Spain

    Francisco Luna

  4. Unidad de Antropología, Departamento de Biología Animal, Universidad de Barcelona, Barcelona, Spain

    Pedro Moral

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  1. Javier Longás
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Correspondence to Antonio Arnaiz-Villena.

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Longás, J., Martínez-Laso, J., Rey, D. et al. Las Alpujarras region (South East Spain) HLA genes study: evidence of a probable success of 17th century repopulation from North Spain. Mol Biol Rep 39, 1387–1394 (2012). https://doi.org/10.1007/s11033-011-0873-4

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