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Genetic Diversity and Geographical Differentiation of Desmodium triflorum (L.) DC. in South China Revealed by AFLP Markers

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Abstract

High levels of genetic variation enable species to adapt to changing environments and provide plant breeders with the raw materials necessary for artificial selection. In the present study, six AFLP primer pairs were used to assess the genetic diversity of Desmodium triflorum (L.) DC. from 12 populations in South China. A high percentage of polymorphic loci (P = 76.16%) and high total gene diversity (H T = 0.310) were found, indicating that the genetic diversity of D. triflorum is high at the species level. Genetic diversity was also relatively high at the population level (P = 55.85%, H e = 0.230). The coefficient of gene differentiation among populations (G ST) was 0.255, indicating that while most genetic diversity resided within populations, there was also considerable differentiation among populations. AMOVA also indicated 24.29% of the total variation to be partitioned among populations (ΦST = 0.243). UPGMA clustering analysis based on genetic distances showed that the 12 populations could be separated into three subgroups: an eastern, a western, and a central-southern subgroup. However, a Mantel test revealed no significant correlation (r = 0.286, p = 0.983) between the geographical distances and genetic distances separating these populations; mountain barriers to gene flow and human disturbance may have confounded these correlations. The present study has provided some fundamental genetic data that will be of use in the exploitation of D. triflorum.

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Acknowledgments

We thank Wei Wu for the technical assistance and also thank Sees-editing Ltd. for the modification of grammar. This research was financially supported by the National Natural Science Foundation of China (30871475), Guangdong natural science fund (9251027501000010, 2005B20901029) of PR China, and Zhang-Hongda Science Foundation of Sun Yat-Sen University.

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Authors and Affiliations

  1. State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, Guangdong, People’s Republic of China

    Mao-feng Yue, Ren-chao Zhou, Ye-lin Huang, Guo-rong Xin & Su-hua Shi

  2. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, People’s Republic of China

    Mao-feng Yue & Li Feng

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  1. Mao-feng Yue
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  2. Ren-chao Zhou
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Correspondence to Guo-rong Xin.

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Yue, Mf., Zhou, Rc., Huang, Yl. et al. Genetic Diversity and Geographical Differentiation of Desmodium triflorum (L.) DC. in South China Revealed by AFLP Markers. J. Plant Biol. 53, 165–171 (2010). https://doi.org/10.1007/s12374-010-9102-2

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