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Predisposition to Nasal Bleeding in Horses

  • ANIMAL HUSBANDRY
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Abstract

The predisposition of horses to nasal bleeding significantly affects their performance and requires special control, particularly in the breeding work aimed at improving the breed and increasing its working abilities. One of the causes of such bleeding is spontaneous nucleotide substitutions in the D'-D3 domain of the von Willebrand factor. The presence and distribution of mutations in the D'-D3 domain of this factor (c.2826 A > C in exon 22, leading to the replacement of lysine by asparagine) in Russian horse breeds (Russian Trotter, Russian Saddle horse, Russian Heavyweight, Altai, and Karachai horse breeds) were analyzed. The highest frequency of this replacement was found in the Russian Trotter and Russian Heavyweight. We aimed to clarify the possible association of this mutation spread with the population and genetic relationship between horse breeds. The polylocus genotyping of 26 genomic DNA fragments flanked by inverted repeats sections of microsatellites (GA)9C, (AG)9C, and (GAG)6C (Inter Simple Sequence Repeats—ISSR-PCR markers) was performed, genetic distances were calculated, and corresponding dendrograms were constructed. It was found that the prevalence of the detected mutation is not related to the population and genetic proximity of the breeds. Historically, the Orlov horse breed had been involved in the breeding of the Russian Trotter and Russian Heavyweight. Therefore, it is possible that the high frequency of this mutation in the Russian Trotter and the Russian Heavyweight may be due to its presence in the Orlov horse breed that was the source for these breeds.

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REFERENCES

  1. Poole, D.C. and Erickson, H.H., Exercise-induced pulmonary hemorrhage: Where are we now?, Vet. Med. Res. Rep., 2016, vol. 7, pp. 133–148.

    Google Scholar 

  2. Tang, W., Cushman, M., Green, D., Rich, S.S., Lange, L.A., Yang, Q., Tracy, R.P., Tofler, G.H., Basu, S., Wilson, J.G., Keating, B.J., Weng, L.C., Taylor, H.A., Jacobs, Jr.D.R., Delaney, J.A., et al., Gene centric approach identifies new and known loci for F VIII activity and VWF antigen levels in European Americans and African Americans, Am. J. Hematol., 2015, vol. 90, no. 6, pp. 534–540.

    Article  CAS  Google Scholar 

  3. Sadler, J.E., Budde, U., Eikenboom, J.C.J., Favaloro, E.J., Hill, F.G.H., Ingerslve, J., Lee, C.A., Lillicrap, D., Mannucci, P.M., Mazurier, C., Meyer, D., Nichols, W.L., Nishino, M., Peake, I.R., Rodeghiero, F., et al., Update on the pathophysiology and classification of von Willebrand disease: A report of the Subcommittee on von Willebrand Factor, J. Thromb. Haemostasis, 2006, vol. 4, no. 10, pp. 2103–2114.

    Article  CAS  Google Scholar 

  4. Norton, E.M., Wooldridge, A.A., Stewart, A.J., Cusimano, L., Schwartz, D.D., Johnson, C.M., Boudreaux, M.K., and Christopherson, P.W., Abnormal coagulation factor VIII transcript in a Tennessee Walking Horse colt with hemophilia A, Vet. Clin. Pathol., 2016, vol. 45, no. 1, pp. 96–102.

    Article  Google Scholar 

  5. Berber, E., Fidanci, I.D., Un, C., Maarri, O., Aktuglu, G., Gurgey, A., Celkan, T., Meral, A., Oldenburg, J., Graw, J., Akar, N., and Caglayan, H., Sequencing of the factor 8 (F8) coding regions in 10 Turkish hemophilia A patients reveals three novel pathological mutations, and one rediagnosis of von Willebrand’s disease type 2N, Haemophilia, 2006, vol. 12, no. 4, pp. 398–400.

    Article  CAS  Google Scholar 

  6. Casonato, A., Galletta, E., Sarolo, L., and Daidone, V., Type 2N von Willebrand disease: Characterization and diagnostic difficulties, Haemophilia, 2018, vol. 24, no. 1, pp. 134–140.

    Article  CAS  Google Scholar 

  7. Hampshire, D.J. and Goodeve, A.C., The international society on thrombosis and haematosis von Willebrand disease database: An update, Sem. Thromb. Hemostasis, 2011, vol. 37, no. 5, pp. 470–479.

    Article  CAS  Google Scholar 

  8. Ginsburg, D. and Sadler, E.J., Von Willebrand disease: A database of point mutations, insertions, and deletions. For the Consortium on von Willebrand Factor Mutations and Polymorphisms, and the Subcommittee on von Willebrand Factor of the Scientific and Standardization Committee of the International Society on Thrombosis and Haemostasis, Thromb. Haemostasis, 1993, vol. 70, no. 2, p. 69.

    Google Scholar 

  9. Kroner, P.A., Friedman, K.D., Fahs, S.A., Scott, J.P., and Montgomery, R.R., Abnormal binding of factor VIII is linked with the substitution of glutamine for arginine 91 in von Willebrand factor in a variant form of von Willebrand disease, J. Biol. Chem., 1991, vol. 266, no. 29, pp. 19146–19149.

    CAS  PubMed  Google Scholar 

  10. Mazurier, C., Goudemand, J., Hilbert, L., Caron, C., Fressinaud, E., and Meyer, D., Type 2N von Willebrand disease: Clinical manifestations, pathophysiology, laboratory diagnosis and molecular biology, Best Pract. Res. Clin. Haematol., 2001, vol. 14, no. 2, pp. 337–347.

    Article  CAS  Google Scholar 

  11. Wang, Q.Y., Song, J., Gibbs, R.A., Boerwinkle, E., Dong, J.F., and Yu, F.L., Characterizing polymorphisms and allelic diversity of von Willebrand factor gene in the 1000 genomes, J. Thromb. Haemostasis, 2013, vol. 11, no. 2, pp. 261–269.

    Article  CAS  Google Scholar 

  12. Trommershausen-Smith, A., Aspects of genetics and disease in the horse, J. Anim. Sci., 1980, vol. 51, no. 5, pp. 1087–1095.

    Article  CAS  Google Scholar 

  13. Brooks, M., Leith, G.S., Allen, A.K., Woods, P.R., Benson, R.E., and Dodds, W.J., Bleeding disorder (von Willebrand disease) in a quarter horse, J. Am. Vet. Med. Assoc., 1991, vol. 198, no. 1, pp. 114–116.

    CAS  PubMed  Google Scholar 

  14. Golik, T.V., Erkenov, T.A., Glazko, T.T., and Glazko, V.I., Spectra of ISSR-PCR markers in estimates of the population-genetic differentiation of the Karachay horse in the farms of the Karachay-Cherkess Republic, Izv. Timiryazevsk. S-kh. Akad., 2018, no. 3, pp. 61–77.

  15. Parfenov, V.A. and Khotov, V.Kh., Gosudarstvennaya plemennaya kniga loshadei karachaevskoi porody (The State Pedigree Book of the Karachay Horse), Moscow: Izd. RGAU-MSKhA im. K. A. Timiryazeva, 2010.

  16. Lobanova, T.V. and Trushnikov, V.A., The Altai horse and the stages of its transformation, Vestn. Altai. Gos. Agrarn. Univ., 2005, no. 1, pp. 84–87.

  17. Kambegov, B.D., Balakshin, O.A., and Khotov, V.Kh., Loshadi Rossii: Polnaya entsiklopediya (Horses of Russia: Complete Encyclopedia), Moscow: RITs MDK, 2002.

  18. Norton, E., Mutational analysis and coagulation factor VIII sequence in a colt with hemophilia A, PhD Thesis, 2013. http://hdl.handle.net/10415/3686.

  19. Zietkiewicz, E., Rafalski, A., and Labuda, D., Genome fingerprinting by secuence repeat (SSR) anchored polymerase chain reaction amplification, Genomics, 1994, vol. 20, no. 2, pp. 176–183.

    Article  CAS  Google Scholar 

  20. Botstein, D., White, R.L., Skolnick, M., and Davis, R.W., Construction of a genetic linkage map in man using restriction fragment length polymorphism, Am. J. Hum. Genet., 1980, vol. 32, no. 3, pp. 314–331.

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Nei Masatoshi, Genetic distance between populations, Am. Nat., 1972, vol. 106, no. 949, pp. 283–292.

    Article  Google Scholar 

  22. Bellissimo, D.B., Christopherson, P.A., Flood, V.H., Gill, J.C., Friedman, K.D., Haberichter, S.L., Shapiro, A.D., Abshire, T.C., Leissinger, C., Hoots, W.K., Lusher, J.M., and Ragni, M.V., VWF mutations and new sequence variations identified in healthy controls are more frequent in the African-American population, Blood, 2012, vol. 119, no. 9, pp. 2135–2140.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Russian State Agrarian University–Timiryazev Moscow Agricultural Academy, 127550, Moscow, Russia

    V. I. Glazko, T. A. Erkenov, R. Alrafy & T. T. Glazko

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  1. V. I. Glazko
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  2. T. A. Erkenov
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  3. R. Alrafy
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  4. T. T. Glazko
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Correspondence to V. I. Glazko.

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Translated by I. Shipounova

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Glazko, V.I., Erkenov, T.A., Alrafy, R. et al. Predisposition to Nasal Bleeding in Horses. Russ. Agricult. Sci. 45, 571–575 (2019). https://doi.org/10.3103/S1068367419060090

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  • DOI: https://doi.org/10.3103/S1068367419060090

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