Nanoladders Facilitate Directional Axonal Outgrowth and Regeneration
- Yimin Huang
Yimin HuangDepartment of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, Massachusetts 02215, United StatesMore by Yimin Huang
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- Ying Jiang
Ying JiangDepartment of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, Massachusetts 02215, United StatesMore by Ying Jiang
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- Qiuyu Wu
Qiuyu WuDepartment of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, Indiana 47907, United StatesMore by Qiuyu Wu
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- Xiangbing Wu
Xiangbing WuStark Neurosciences Research Institute, Indiana University School of Medicine, 320 W. 15th Street, Indianapolis, Indiana 46202, United StatesMore by Xiangbing Wu
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- Xingda An
Xingda AnDepartment of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, Massachusetts 02215, United StatesMore by Xingda An
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- Alexander A. Chubykin
Alexander A. ChubykinDepartment of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, Indiana 47907, United StatesMore by Alexander A. Chubykin
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- Ji-Xin Cheng*
Ji-Xin Cheng*E-mail: [email protected]Department of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, Massachusetts 02215, United StatesDepartment of Electrical and Computer Engineering, Boston University, 8 St Mary’s Street, Boston, Massachusetts 02215, United StatesDepartment of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, Massachusetts 02215, United StatesMore by Ji-Xin Cheng
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- Xiao-Ming Xu*
Xiao-Ming Xu*E-mail: [email protected]Stark Neurosciences Research Institute, Indiana University School of Medicine, 320 W. 15th Street, Indianapolis, Indiana 46202, United StatesMore by Xiao-Ming Xu
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- Chen Yang*
Chen Yang*E-mail: [email protected]Department of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, Massachusetts 02215, United StatesDepartment of Electrical and Computer Engineering, Boston University, 8 St Mary’s Street, Boston, Massachusetts 02215, United StatesMore by Chen Yang
Abstract
After injuries, axonal regeneration over long distance is challenging due to lack of orientation guidance. Biocompatible scaffolds have been used to mimic the native organization of axons to guide and facilitate axonal regeneration. Those scaffolds are of great importance in achieving functional connections of the nervous system. We have developed a nanoladder scaffold to guide directional outgrowth and facilitate regeneration of axons. The nanoladders, composed of micron-scale stripes and nanoscale protrusions, were fabricated on the glass substrate using photolithography and reactive ion etching methods. Embryonic neurons cultured on the nanoladder scaffold showed significant neurite elongation and axonal alignment in parallel with the nanoladder direction. Furthermore, the nanoladders promoted axonal regeneration and functional connection between organotypic spinal cord slices over 1 mm apart. Multimodality imaging studies revealed that such neuronal regeneration was supported by directional outgrowth of glial cells along nanoladders in the organotypic spinal cord slice culture as well as in the coculture of glial cells and neurons. These results collectively herald the potential of our nanoladder scaffold in facilitating and guiding neuronal development and functional restoration.
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