Microbiology and Immunology

Volume 45, Issue 3 p. 201-208

Original Article

Free Access

Effect of Sealing and Tween 80 on the Antifungal Susceptibility Testing of Essential Oils

Shigeharu Inouye,

Corresponding Author

Shigeharu Inouye

Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, 192-0395 Japan

Address correspondence to Dr. Shigeharu Inouye, Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192–0395, Japan. Fax: +81–426–74–9190.Search for more papers by this author

Tsutomu Tsuruoka,

Tsutomu Tsuruoka

Meiji Seika Kaisha, Ltd., Chuo-ku, Tokyo, 104-8002 Japan

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Katsuhisa Uchida,

Katsuhisa Uchida

Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, 192-0395 Japan

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Hideyo Yamaguchi,

Hideyo Yamaguchi

Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, 192-0395 Japan

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Shigeharu Inouye,

Corresponding Author

Shigeharu Inouye

Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, 192-0395 Japan

Tsutomu Tsuruoka,

Tsutomu Tsuruoka

Meiji Seika Kaisha, Ltd., Chuo-ku, Tokyo, 104-8002 Japan

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Katsuhisa Uchida,

Katsuhisa Uchida

Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, 192-0395 Japan

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Hideyo Yamaguchi,

Hideyo Yamaguchi

Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, 192-0395 Japan

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First published: 14 November 2013

https://doi.org/10.1111/j.1348-0421.2001.tb02608.x

Citations: 47

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Abstract

Concentrations of essential oils showing high volatility decreased substantially in broth and agar media when incubated under open conditions. The decrease in the half life was from 0.7 to 38 hr in broth medium at 27 C. When evaporation was prevented by sealing, MIC values against Aspergillus fumigatus, Candida albicans and Trichophyton mentagrophytes by broth or agar dilution assay were lowered two to eight-fold, as compared with those obtained under open conditions. Addition of Tween 80 caused a rise of the MICs against A. fumigatus by two to four-fold in broth dilution assay, but little affected the MICs in agar dilution assay.

Abbreviations

CFU: colony forming unit
DMSO: dimethyl-sulfoxide
GC: gas chromatography
MFC: minimum fungicidal concentration
MIC: minimum inhibitory concentration
NCCLS: National Committee for Clinical Laboratory Standards.

References

1Adam, K., Sivropoulon, A., Kokkini, S., Lanaras, T., and Arsenakis, M. 1998. Antifungal activities of Origanum vulgare subsp. Hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi. J. Agric. Food Chem. 46: 1739–1745.
10.1021/jf9708296
CASWeb of Science®Google Scholar
2Begum, J., Yusuf, M., Chowdhury, T.U., and Wahabs, M.A. 1993. Studies on essential oils for their antibacterial and antifungal properties. Part 1. Preliminary screening of 35 essential oils. Bangladesh J. Sci. Ind. Res. 28: 25–34.

CASGoogle Scholar
3Carson, C.F., Hammer, K.A., and Riley, T.V. 1995. Broth micro-dilution method for determining the susceptibility of Escherichia coli and Staphylococcus aureus to the essential oil of Melaleuca alternifolia (tea tree oil). Microbios 82: 181–185.

CASPubMedWeb of Science®Google Scholar
4Carson, C.F., and Riley, T.V. 1995. Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. J. Appl. Bacteriol. 78: 264–269.
10.1111/j.1365-2672.1995.tb05025.x
CASPubMedWeb of Science®Google Scholar
5Chalchat, J.C., Garry, R.P., Bastide, P., Fabre, F., and Malhuret, R. 1991. Chemical composition/antimicrobial activity correlation. V. Comparison of two methods for MIC determinations. Plant. Med. Phytother. 25: 184–193.

CASGoogle Scholar
6Chou, C.K.S., and Zabkiewicz, J.A. 1976. Toxicity of monoterpenes from Pinus radiata corticaloleoresin to Diplodia pinea spores. Eur. J. For. Pathol. 6: 354–359.
10.1111/j.1439-0329.1976.tb00549.x
CASWeb of Science®Google Scholar
7Faraz, R.S., Daw, Z.Y., Hewedi, F.M., and El-Baroty, G.S.A. 1989. Antimicrobial activity of some Egyptian spice essential oils. J. Food Prot. 52: 665–667.

Google Scholar
8Goi, H., Inouye, S., and Iwanami, Y. 1985. Antifungal activity of powdery black mustard, powdery Wasabi (Japanese horseradish) and allyl isothiocyanate by gaseous contact. J. Antib. Antifung. Agents 13: 199–204.

Google Scholar
9Hammer, K.A., Carson, C.F., and Riley, T.V. 1998. In-vitro activity of essential oils, in particular Melaleuca alternifolia (tea tree) oil and tea tree oil products against Candida spp. J. Antimicrob. Chemother. 42: 591–595.
10.1093/jac/42.5.591
CASPubMedWeb of Science®Google Scholar
10Inouye, S., Watanabe, M., Nishiyama, Y., Takeo, K., Akao, M., and Yamaguchi, H. 2000. Inhibitory effect of essential oils on apical growth of Aspergillus fumigatus by vapour contact. Mycoses 43: 17–23.
10.1046/j.1439-0507.2000.00538.x
CASPubMedWeb of Science®Google Scholar
11Janssen, A.M., Scheffer, J.J.C., and Svendsen, A.B. 1987. Antimicrobial activity of essential oils: a 1976–1986 literature review. Aspects of the test methods. Planta Medica: 395–398.

Google Scholar
12Janssen, A.M., Scheffer, J.J.C., and Svendsen, A.B. 1986. Antimicrobial screening of essential oils. Aspects of the agar overlay technique. Prog. Essent. Oil Rec. Proc. Int. Symp. Essent. Oils, 16^th: 401–419, Berlin.

Google Scholar
13Juven, B.J., Kanner, J., Schved, F., and Weisslowicz, H. 1994. Factors that interact with the antibacterial action of thyme essential oil and its active constituents. J. Appl. Bacteriol. 76: 626–631.
10.1111/j.1365-2672.1994.tb01661.x
CASPubMedWeb of Science®Google Scholar
14Kazmi, S.J.A., and Mitchell, A.G. 1978. Preservation of solubilized and emulsified systems II: the theoretical development of capacity and its role in antimicrobial activity of chlorocresol in cetomacrogal-stabilized systems. J. Pharmaceut. Sci. 67: 1266–1271.
10.1002/jps.2600670920
CASPubMedWeb of Science®Google Scholar
15Knobloch, K., Pauli, A., Iberl, B., Weigand, H., and Weis, N. 1989. Antibacterial and antifungal properties of essential oil components. J. Essent. Oil Res. 1: 119–128.
10.1080/10412905.1989.9697767
CASGoogle Scholar
16Kurita, N., Miyaji, M., Kurane, R., Takahara, Y., and Ichimaru, K. 1979. Antifungal activity and molecular orbital energies of aldehyde compounds from oils of higher plants. Agric. Biol. Chem. 43: 2365–2371.
10.1271/bbb1961.43.2365
CASWeb of Science®Google Scholar
17Lis-Balchin, M., Deans, S.G., and Eaglesham, E. 1998. Relationship between bioactivity and chemical composition of commercial essential oils. Flavour Fragrance J. 13: 98–104.
10.1002/(SICI)1099-1026(199803/04)13:2<98::AID-FFJ705>3.0.CO;2-B
CASWeb of Science®Google Scholar
18Makimura, K., Sudo, T., Kudo, M., Uchida, K., and Yamaguchi, H. 1998. Development of reference procedures for broth microdilution antifungal susceptibility testing of yeast with standardized end point determination. Microbiol. Immunol. 42: 55–59.
10.1111/j.1348-0421.1998.tb01970.x
CASPubMedWeb of Science®Google Scholar
19Maruzzella, J.C., Baiter, J., and Katz, A. 1959. The action of perfume-oil vapors on fungi. Am. Perfumer Aromat. 74: 21–22.

CASGoogle Scholar
20Maruzzella, J.C., and Ligouri, L. 1958. The in vitro antifungal activity of essential oils. J. Am. Pharm. Assoc. 47: 250–254.
10.1002/jps.3030470407
CASPubMedWeb of Science®Google Scholar
21Moleyar, V., and Narasimham, P. 1986. Antifungal activity of some essential oil components. Food Microbiol. 3: 331–336.
10.1016/0740-0020(86)90017-1
CASGoogle Scholar
22 National Committee for Clinical Laboratory Standards. 1998. Reference method for broth dilution antifungal susceptibility testing of conidium-forming filamentous fungi; proposed standard. Document M38-P. National Committee for Clinical Laboratory Standard, Villanova, Pa.

Google Scholar
23Nenoff, P., Haustein, U.-F., and Brandt, W. 1996. Antifungal activity of the essential oil of Melaleuca alternifolia (Tea tree oil) against pathogenic fungi in vitro. Skin Pharmacol. 9: 388–394.
10.1159/000211450
CASPubMedWeb of Science®Google Scholar
24Pandey, D.K., Tripathi, N.N., Tripathi, R.D., and Dixit, S.N. 1982. Fungitoxic and phytotoxic properties of the essential oil of Caesulia axillaris Roxb. (Compositae). Angew. Botanik 56: 259–267.

Web of Science®Google Scholar
25Remmal, A., Bouchikhi, T., Rheyour, K., Ettayebi, M., and Tantaoui-Elaraki, A. 1993. Improved method for the determination of antimicrobial activity of essential oils in agar medium. J. Essent. Oil Res. 5: 179–184.
10.1080/10412905.1993.9698197
CASGoogle Scholar
26Sadafian, A., and Crouzet, J. 1987. Infinite dilution activity coefficients and relative volatilities of some aroma compounds. Flavour Fragrance J. 2: 103–107.
10.1002/ffj.2730020305
CASGoogle Scholar
27Singh, H.B., Srivastava, M., Singh, A.B., and Srivastava, A.K. 1995. Cinnamon bark oil, a potent fungi toxicant against fungi causing respiratory tract mycoses. Allergy 50: 995–999.
10.1111/j.1398-9995.1995.tb02515.x
CASPubMedWeb of Science®Google Scholar
28Steinmetz, M.D., Moulin-Traffort, J., and Regli, P. 1988. Transmission and scanning electron-microscopy study of the action of sage and rosemary essential oils and eucalyptol on Candida albicans. Mycoses 31: 40–51.
10.1111/j.1439-0507.1988.tb03856.x
CASPubMedWeb of Science®Google Scholar
29Wilson, C.L., Solar, J.M., El Ghaouth, A., and Wisinieuski, M.E. 1987. Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinereu. Plant Dis. 81: 204–210.
10.1094/PDIS.1997.81.2.204
Web of Science®Google Scholar
30Yegen, O., Berger, B., and Heitefuss, R. 1992. Investigation on the fungitoxicity of extracts of six selected plants from Turkey against phytopathogenic fungi. Z. Pflanzenkrankh. Pflanzenschutz. 99: 349–359.

CASWeb of Science®Google Scholar

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Volume45, Issue3

March 2001

Pages 201-208

Effect of Sealing and Tween 80 on the Antifungal Susceptibility Testing of Essential Oils

Abstract

Abbreviations

References

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References

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Effect of Sealing and Tween 80 on the Antifungal Susceptibility Testing of Essential Oils

Abstract

Abbreviations

References

Citing Literature

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