Isolation and Characterization of Viruses Related to the SARS Coronavirus from Animals in Southern China
Abstract
A novel coronavirus (SCoV) is the etiological agent of severe acute respiratory syndrome (SARS). SCoV-like viruses were isolated from Himalayan palm civets found in a live-animal market in Guangdong, China. Evidence of virus infection was also detected in other animals (including a raccoon dog, Nyctereutes procyonoides) and in humans working at the same market. All the animal isolates retain a 29-nucleotide sequence that is not found in most human isolates. The detection of SCoV-like viruses in small, live wild mammals in a retail market indicates a route of interspecies transmission, although the natural reservoir is not known.
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References and Notes
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We thank the Department of Health and Department of Agriculture of Shenzhen Government for facilitating the study. We gratefully acknowledge the encouragement and support of L. C. Tsui, Vice-Chancellor, The University of Hong Kong. We thank X. Y. Zhao from the Department of the Microbiology, The University of Hong Kong, for the excellent technical assistance. We also thank C. C. Hon and F. C. Leung from the Department of Zoology, The University of Hong Kong, and Richard Webby from St. Jude Children's Research Hospital (Memphis, TN, U.S.A.) for assistance in the phylogenetic analysis. We thank K. V. Holmes's laboratory from the Department of Microbiology, University of Colorado Health Sciences Center (Denver, CO, U.S.A.) to validate the animal viral sequences. Supported by research funding from Public Health Research (Grant A195357), the U.S. National Institute of Allergy and Infectious Diseases, the Wellcome Trust (067072/D/02/Z), and SARS research funds from The University of Hong Kong.
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Science
Volume 302 | Issue 5643
10 October 2003
10 October 2003
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American Association for the Advancement of Science.
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Submission history
Received: 22 May 2003
Accepted: 26 August 2003
Published in print: 10 October 2003
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