Abstract
A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
Publication types
- Comparative Study
- Evaluation Study
MeSH terms
- Animals
- Base Sequence
- Chlorocebus aethiops
- DNA Primers / genetics
- DNA, Viral / genetics
- Humans
- RNA, Viral / genetics
- RNA, Viral / isolation & purification
- Reproducibility of Results
- Reverse Transcriptase Polymerase Chain Reaction* / statistics & numerical data
- Sensitivity and Specificity
- Severe Acute Respiratory Syndrome / diagnosis
- Severe Acute Respiratory Syndrome / epidemiology
- Severe Acute Respiratory Syndrome / virology
- Severe acute respiratory syndrome-related coronavirus / genetics*
- Severe acute respiratory syndrome-related coronavirus / isolation & purification*
- Vero Cells
Substances
- DNA Primers
- DNA, Viral
- RNA, Viral