SARS-CoV-2-encoded nucleocapsid protein acts as a viral suppressor of RNA interference in cells

Jingfang Mu; Jiuyue Xu; Leike Zhang; Ting Shu; Di Wu; Muhan Huang; Yujie Ren; Xufang Li; Qing Geng; Yi Xu; Yang Qiu; Xi Zhou

doi:10.1007/s11427-020-1692-1

SARS-CoV-2-encoded nucleocapsid protein acts as a viral suppressor of RNA interference in cells

Sci China Life Sci. 2020 Sep;63(9):1413-1416. doi: 10.1007/s11427-020-1692-1. Epub 2020 Apr 10.

Authors

Jingfang Mu^{1 2}, Jiuyue Xu^{1 3}, Leike Zhang¹, Ting Shu^{1 2 4}, Di Wu^{1 2}, Muhan Huang^{1 2}, Yujie Ren^{1 5}, Xufang Li⁵, Qing Geng⁶, Yi Xu⁵, Yang Qiu^{7 8 9}, Xi Zhou^{10 11 12 13}

Affiliations

¹ State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences (CAS), Wuhan, 430071, China.
² Joint Laboratory of Infectious Diseases and Health, Wuhan Institute of Virology & Wuhan Jinyintan Hospital, CAS, Wuhan, 430023, China.
³ The University of Chinese Academy of Sciences, Beijing, 100049, China.
⁴ Center for Translational Medicine, Wuhan Jinyintan Hospital, Wuhan, 430040, China.
⁵ Center for Precision Translational Medicine of Wuhan Institute of Virology and Guangzhou Women and Children's Medical Center, Guangzhou Women and Children's Medical Center, Guangzhou, 510120, China.
⁶ Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
⁷ State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences (CAS), Wuhan, 430071, China. zhouxi@wh.iov.cn.
⁸ Joint Laboratory of Infectious Diseases and Health, Wuhan Institute of Virology & Wuhan Jinyintan Hospital, CAS, Wuhan, 430023, China. zhouxi@wh.iov.cn.
⁹ The University of Chinese Academy of Sciences, Beijing, 100049, China. zhouxi@wh.iov.cn.
¹⁰ State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences (CAS), Wuhan, 430071, China. yangqiu@wh.iov.cn.
¹¹ Joint Laboratory of Infectious Diseases and Health, Wuhan Institute of Virology & Wuhan Jinyintan Hospital, CAS, Wuhan, 430023, China. yangqiu@wh.iov.cn.
¹² The University of Chinese Academy of Sciences, Beijing, 100049, China. yangqiu@wh.iov.cn.
¹³ Center for Translational Medicine, Wuhan Jinyintan Hospital, Wuhan, 430040, China. yangqiu@wh.iov.cn.

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Betacoronavirus / genetics*
Betacoronavirus / pathogenicity
Betacoronavirus / physiology
COVID-19
Coronavirus Infections / genetics*
Coronavirus Infections / virology*
Coronavirus Nucleocapsid Proteins
DEAD-box RNA Helicases / metabolism
Green Fluorescent Proteins / antagonists & inhibitors
Green Fluorescent Proteins / genetics
HEK293 Cells
Humans
Nucleocapsid Proteins / genetics*
Nucleocapsid Proteins / physiology
Pandemics
Phosphoproteins
Pneumonia, Viral / genetics*
Pneumonia, Viral / virology*
RNA Interference*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Small Interfering / genetics
RNA-Binding Proteins / genetics
RNA-Binding Proteins / physiology
Ribonuclease III / metabolism
SARS-CoV-2
Transfection

Substances

Coronavirus Nucleocapsid Proteins
Nucleocapsid Proteins
Phosphoproteins
RNA, Messenger
RNA, Small Interfering
RNA-Binding Proteins
enhanced green fluorescent protein
nucleocapsid phosphoprotein, SARS-CoV-2
Green Fluorescent Proteins
DICER1 protein, human
Ribonuclease III
DEAD-box RNA Helicases